Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine
A high-performance liquid chromatography (HPLC) in combination with inductively coupled plasma mass spectrometry (ICP-MS) as an elemental specific detector was used for the speciation analysis of arsenic compounds in urine and serum samples from Vietnam. Five arsenic species including arsenite (AsII...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/9462019 |
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| author | Manh Ha Nguyen Tien Duc Pham Thi Lien Nguyen Hai Anh Vu Thi Thao Ta Minh Binh Tu Thi Hong Yen Nguyen Dinh Binh Chu |
| author_facet | Manh Ha Nguyen Tien Duc Pham Thi Lien Nguyen Hai Anh Vu Thi Thao Ta Minh Binh Tu Thi Hong Yen Nguyen Dinh Binh Chu |
| author_sort | Manh Ha Nguyen |
| collection | DOAJ |
| description | A high-performance liquid chromatography (HPLC) in combination with inductively coupled plasma mass spectrometry (ICP-MS) as an elemental specific detector was used for the speciation analysis of arsenic compounds in urine and serum samples from Vietnam. Five arsenic species including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), and arsenobetaine (AsB) were studied. A gradient elution of ammonium carbonate ((NH4)2CO3), ethylenediaminetetraacetic acid disodium salt (Na2EDTA), and methanol at pH 9.0 utilizing Hamilton PRP-X100 strong anion-exchange column allowed the chromatographic separation of five arsenic species. In this study, urine and serum samples were prepared by dilution in solvent and protein precipitation by trichloroacetic acid, respectively. The extraction efficiency was greater than 91% for urine matrix, and recoveries from spiked samples were in the range of 94–139% for the arsenic species in human serum. The method limit of detection (MDL) and limit of quantification (MQL), which were calculated by signal to noise ratio, were found to be 0.3–1.5 and 1.0–5.0 ng·mL−1, respectively. The concentration of arsenic species in 17 pairs of urine and serum samples from Vietnam was also quantified and evaluated. The major species of arsenic in the urine and serum samples were AsB and DMA. |
| format | Article |
| id | doaj-art-e3b3c5f144f44695aa47da26f3f39f96 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e3b3c5f144f44695aa47da26f3f39f962025-08-20T02:21:24ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/94620199462019Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and UrineManh Ha Nguyen0Tien Duc Pham1Thi Lien Nguyen2Hai Anh Vu3Thi Thao Ta4Minh Binh Tu5Thi Hong Yen Nguyen6Dinh Binh Chu7Faculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamFaculty of Chemistry, VNU University of Science, Vietnam National University-Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi 100000, VietnamNational Institute of Hygiene and Epidemiology, 1 Yersin, Hai Ba Trung, Hanoi 100000, VietnamSchool of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Hanoi 100000, VietnamA high-performance liquid chromatography (HPLC) in combination with inductively coupled plasma mass spectrometry (ICP-MS) as an elemental specific detector was used for the speciation analysis of arsenic compounds in urine and serum samples from Vietnam. Five arsenic species including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), and arsenobetaine (AsB) were studied. A gradient elution of ammonium carbonate ((NH4)2CO3), ethylenediaminetetraacetic acid disodium salt (Na2EDTA), and methanol at pH 9.0 utilizing Hamilton PRP-X100 strong anion-exchange column allowed the chromatographic separation of five arsenic species. In this study, urine and serum samples were prepared by dilution in solvent and protein precipitation by trichloroacetic acid, respectively. The extraction efficiency was greater than 91% for urine matrix, and recoveries from spiked samples were in the range of 94–139% for the arsenic species in human serum. The method limit of detection (MDL) and limit of quantification (MQL), which were calculated by signal to noise ratio, were found to be 0.3–1.5 and 1.0–5.0 ng·mL−1, respectively. The concentration of arsenic species in 17 pairs of urine and serum samples from Vietnam was also quantified and evaluated. The major species of arsenic in the urine and serum samples were AsB and DMA.http://dx.doi.org/10.1155/2018/9462019 |
| spellingShingle | Manh Ha Nguyen Tien Duc Pham Thi Lien Nguyen Hai Anh Vu Thi Thao Ta Minh Binh Tu Thi Hong Yen Nguyen Dinh Binh Chu Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine Journal of Analytical Methods in Chemistry |
| title | Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine |
| title_full | Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine |
| title_fullStr | Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine |
| title_full_unstemmed | Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine |
| title_short | Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine |
| title_sort | speciation analysis of arsenic compounds by hplc icp ms application for human serum and urine |
| url | http://dx.doi.org/10.1155/2018/9462019 |
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