The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
Introduction. The pathogenesis of GERD is strongly related with mixed acid and bile reflux. Benign and malignant esophageal and gastric lesions have been associated with synergetic activity between those parame...
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| Format: | Article |
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Wiley
2011-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2011/905872 |
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| author | Andreas Nakos Georgios Kouklakis Michail Pitiakoudis Petros Zezos Eleni Efraimidou Alexandra Giatromanolaki Alexandros Polychronidis Nikolaos Liratzopoulos Efthimios Sivridis Konstantinos Simopoulos |
| author_facet | Andreas Nakos Georgios Kouklakis Michail Pitiakoudis Petros Zezos Eleni Efraimidou Alexandra Giatromanolaki Alexandros Polychronidis Nikolaos Liratzopoulos Efthimios Sivridis Konstantinos Simopoulos |
| author_sort | Andreas Nakos |
| collection | DOAJ |
| description | Introduction. The pathogenesis of GERD is strongly
related with mixed acid and bile reflux. Benign and malignant
esophageal and gastric lesions have been associated with synergetic
activity between those parameters. Bile reflux causes reactive
gastropathy evaluated with Bile Reflux Index (BRI). The aim was to
investigate if the sequence: bile reflux-intestinal
metaplasia-GERD-esophagitis, is associated with
apoptotic/oncogenetic disturbances.
Materials/Methods. Fifteen asymptomatic subjects and
53 GERD patients underwent gastroscopy with biopsies. The
specimens examined histologically and immunohistochemically for
p53, Ki-67, Bax, and Bcl-2. Results.
Elevated BRI score detected in 47% (25/53) of patients with GERD
and in 13% (2/15) of controls (𝑃=0.02). Severe esophageal lesions were significantly more common
in BRI (+) patients (14/25) compared to BRI (−) ones (𝑃=0.0049). Immunohistochemical analysis did not show associations
between BRI score and biomarker expression.
Conclusions. Bile reflux gastropathy is
associated with GERD severity, but not with oncogene expression or
apoptotic discrepancies of the upper GI mucosa. |
| format | Article |
| id | doaj-art-e3ab44274bbe485fa4f08ce7ab1b14cd |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-e3ab44274bbe485fa4f08ce7ab1b14cd2025-08-20T03:26:31ZengWileyGastroenterology Research and Practice1687-61211687-630X2011-01-01201110.1155/2011/905872905872The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux DiseaseAndreas Nakos0Georgios Kouklakis1Michail Pitiakoudis2Petros Zezos3Eleni Efraimidou4Alexandra Giatromanolaki5Alexandros Polychronidis6Nikolaos Liratzopoulos7Efthimios Sivridis8Konstantinos Simopoulos9Gastrointestinal Endoscopy Unit, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreeceGastrointestinal Endoscopy Unit, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece2nd Department of Surgery, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreeceGastrointestinal Endoscopy Unit, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece1st Department of Surgery, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreecePathology Department, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreecePathology Department, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece1st Department of Surgery, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreecePathology Department, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece2nd Department of Surgery, Democritus University of Thrace, University General Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, GreeceIntroduction. The pathogenesis of GERD is strongly related with mixed acid and bile reflux. Benign and malignant esophageal and gastric lesions have been associated with synergetic activity between those parameters. Bile reflux causes reactive gastropathy evaluated with Bile Reflux Index (BRI). The aim was to investigate if the sequence: bile reflux-intestinal metaplasia-GERD-esophagitis, is associated with apoptotic/oncogenetic disturbances. Materials/Methods. Fifteen asymptomatic subjects and 53 GERD patients underwent gastroscopy with biopsies. The specimens examined histologically and immunohistochemically for p53, Ki-67, Bax, and Bcl-2. Results. Elevated BRI score detected in 47% (25/53) of patients with GERD and in 13% (2/15) of controls (𝑃=0.02). Severe esophageal lesions were significantly more common in BRI (+) patients (14/25) compared to BRI (−) ones (𝑃=0.0049). Immunohistochemical analysis did not show associations between BRI score and biomarker expression. Conclusions. Bile reflux gastropathy is associated with GERD severity, but not with oncogene expression or apoptotic discrepancies of the upper GI mucosa.http://dx.doi.org/10.1155/2011/905872 |
| spellingShingle | Andreas Nakos Georgios Kouklakis Michail Pitiakoudis Petros Zezos Eleni Efraimidou Alexandra Giatromanolaki Alexandros Polychronidis Nikolaos Liratzopoulos Efthimios Sivridis Konstantinos Simopoulos The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease Gastroenterology Research and Practice |
| title | The Histological and Immunohistochemical Aspects of Bile
Reflux in Patients with Gastroesophageal Reflux
Disease |
| title_full | The Histological and Immunohistochemical Aspects of Bile
Reflux in Patients with Gastroesophageal Reflux
Disease |
| title_fullStr | The Histological and Immunohistochemical Aspects of Bile
Reflux in Patients with Gastroesophageal Reflux
Disease |
| title_full_unstemmed | The Histological and Immunohistochemical Aspects of Bile
Reflux in Patients with Gastroesophageal Reflux
Disease |
| title_short | The Histological and Immunohistochemical Aspects of Bile
Reflux in Patients with Gastroesophageal Reflux
Disease |
| title_sort | histological and immunohistochemical aspects of bile reflux in patients with gastroesophageal reflux disease |
| url | http://dx.doi.org/10.1155/2011/905872 |
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