Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating

We have improved the water-holding capacity and gelation properties of egg white gels by combining glycosylation with alkaline-heating. A comprehensive evaluation was conducted, encompassing parameters such as color, texture, water-holding capacity (WHC), intermolecular forces, secondary structure,...

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Main Authors: Yang Ye, Xinyue Gou, Guixiang Jia, Jingyu Xiang, Anjiao Li, Yang Wang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:International Journal of Food Properties
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/10942912.2025.2471969
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author Yang Ye
Xinyue Gou
Guixiang Jia
Jingyu Xiang
Anjiao Li
Yang Wang
author_facet Yang Ye
Xinyue Gou
Guixiang Jia
Jingyu Xiang
Anjiao Li
Yang Wang
author_sort Yang Ye
collection DOAJ
description We have improved the water-holding capacity and gelation properties of egg white gels by combining glycosylation with alkaline-heating. A comprehensive evaluation was conducted, encompassing parameters such as color, texture, water-holding capacity (WHC), intermolecular forces, secondary structure, and gel electrophoresis. The findings revealed that the glycosylation reaction by reducing the carbonyl group of the sugar and the amino group of the protein significantly influenced the gel’s color, yielding a distinctive reddish-brown hue. Additionally, the incorporation of glucose enhanced the WHC of the composite gel to 90%. The gel’s hardness exhibited a notable increase from 170 g to 379 g with the addition of 8% arabinose, while its elasticity improved to 2.9. Furthermore, chewiness increased from 227 g to 740 g upon the addition of 8% lactose. The glycosylation reaction facilitated a structural transition in the proteins, characterized by the conversion of β-sheets into random coils. Microstructural analysis indicated a shift from an ordered yet loose and porous architecture to a disordered, heterogeneous lamellar structure. The degree of crosslinking within the gel was augmented, and SDS-PAGE results corroborated the occurrence of protein aggregation. This study establishes a theoretical foundation for advancing the quality and processing techniques of preserved eggs.
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issn 1094-2912
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publishDate 2025-12-01
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series International Journal of Food Properties
spelling doaj-art-e3a8f5db2d524c079287bd833621854a2025-08-20T03:05:22ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862025-12-0128110.1080/10942912.2025.2471969Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heatingYang Ye0Xinyue Gou1Guixiang Jia2Jingyu Xiang3Anjiao Li4Yang Wang5School of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaSchool of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaSchool of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaSchool of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaSchool of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaSchool of Biological Engineering, Sichuan University of Science and Engineering, Yibin, Sichuan, ChinaWe have improved the water-holding capacity and gelation properties of egg white gels by combining glycosylation with alkaline-heating. A comprehensive evaluation was conducted, encompassing parameters such as color, texture, water-holding capacity (WHC), intermolecular forces, secondary structure, and gel electrophoresis. The findings revealed that the glycosylation reaction by reducing the carbonyl group of the sugar and the amino group of the protein significantly influenced the gel’s color, yielding a distinctive reddish-brown hue. Additionally, the incorporation of glucose enhanced the WHC of the composite gel to 90%. The gel’s hardness exhibited a notable increase from 170 g to 379 g with the addition of 8% arabinose, while its elasticity improved to 2.9. Furthermore, chewiness increased from 227 g to 740 g upon the addition of 8% lactose. The glycosylation reaction facilitated a structural transition in the proteins, characterized by the conversion of β-sheets into random coils. Microstructural analysis indicated a shift from an ordered yet loose and porous architecture to a disordered, heterogeneous lamellar structure. The degree of crosslinking within the gel was augmented, and SDS-PAGE results corroborated the occurrence of protein aggregation. This study establishes a theoretical foundation for advancing the quality and processing techniques of preserved eggs.https://www.tandfonline.com/doi/10.1080/10942912.2025.2471969Egg white gelglycosylationcolorgel propertiesprotein secondary structure
spellingShingle Yang Ye
Xinyue Gou
Guixiang Jia
Jingyu Xiang
Anjiao Li
Yang Wang
Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
International Journal of Food Properties
Egg white gel
glycosylation
color
gel properties
protein secondary structure
title Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
title_full Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
title_fullStr Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
title_full_unstemmed Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
title_short Improving the water-holding and gelation properties of egg white by glycosylation and alkaline heating
title_sort improving the water holding and gelation properties of egg white by glycosylation and alkaline heating
topic Egg white gel
glycosylation
color
gel properties
protein secondary structure
url https://www.tandfonline.com/doi/10.1080/10942912.2025.2471969
work_keys_str_mv AT yangye improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating
AT xinyuegou improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating
AT guixiangjia improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating
AT jingyuxiang improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating
AT anjiaoli improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating
AT yangwang improvingthewaterholdingandgelationpropertiesofeggwhitebyglycosylationandalkalineheating