EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL

Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solute...

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Main Author: M.R. Hadjmohammadi
Format: Article
Language:English
Published: University of Tehran 2003-12-01
Series:Journal of Sciences, Islamic Republic of Iran
Subjects:
Online Access:https://jsciences.ut.ac.ir/article_35093_157fe4b3a13009fb1abcfe003b57cccd.pdf
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author M.R. Hadjmohammadi
author_facet M.R. Hadjmohammadi
author_sort M.R. Hadjmohammadi
collection DOAJ
description Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed that the variation of percentages of organic modifier (5-20% V/V CH3CN) changes cavity formation (m = +3.55 to +2.53), dipole-dipole inter­actions (s = +0.62 to −0.64) and hydrogen-bond acidity (b = −2.41 to −3.27) terms. The variation of temperature (5-45°C) also changes cavity formation (m = +3.21 to +2.56) and dipole-dipole interactions (s = +0.34 to −0.22) terms.
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publishDate 2003-12-01
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series Journal of Sciences, Islamic Republic of Iran
spelling doaj-art-e3a88ceab9064f47832fc2f3344b10942025-08-20T03:53:51ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142003-12-0114433734035093EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODELM.R. Hadjmohammadi0Reversed-phase liquid chromatography; Solvation parameter modelEffects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed that the variation of percentages of organic modifier (5-20% V/V CH3CN) changes cavity formation (m = +3.55 to +2.53), dipole-dipole inter­actions (s = +0.62 to −0.64) and hydrogen-bond acidity (b = −2.41 to −3.27) terms. The variation of temperature (5-45°C) also changes cavity formation (m = +3.21 to +2.56) and dipole-dipole interactions (s = +0.34 to −0.22) terms.https://jsciences.ut.ac.ir/article_35093_157fe4b3a13009fb1abcfe003b57cccd.pdfreversed-phase liquid chromatographysolvation parameter model
spellingShingle M.R. Hadjmohammadi
EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
Journal of Sciences, Islamic Republic of Iran
reversed-phase liquid chromatography
solvation parameter model
title EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
title_full EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
title_fullStr EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
title_full_unstemmed EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
title_short EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
title_sort effects of temperature and percentage of organic modifier on retention and selectivity in rp hplc using solvation parameter model
topic reversed-phase liquid chromatography
solvation parameter model
url https://jsciences.ut.ac.ir/article_35093_157fe4b3a13009fb1abcfe003b57cccd.pdf
work_keys_str_mv AT mrhadjmohammadi effectsoftemperatureandpercentageoforganicmodifieronretentionandselectivityinrphplcusingsolvationparametermodel