Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress
Abstract The study’s goals are to fabricate PLGA nanoparticles (PNPs) loaded with apigenin (AP) and adenosine (AD) using a microfluidic preparation method to a standard emulsification method and investigate the possible heart-protective effects of AP-AD PNPs made using the emulsification method. Com...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | BMC Biotechnology |
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| Online Access: | https://doi.org/10.1186/s12896-024-00912-y |
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| author | Ruixuan Li Aixia Xu Ye Chen Yihui Li Ru Fu Weihong Jiang Xiaogang Li |
| author_facet | Ruixuan Li Aixia Xu Ye Chen Yihui Li Ru Fu Weihong Jiang Xiaogang Li |
| author_sort | Ruixuan Li |
| collection | DOAJ |
| description | Abstract The study’s goals are to fabricate PLGA nanoparticles (PNPs) loaded with apigenin (AP) and adenosine (AD) using a microfluidic preparation method to a standard emulsification method and investigate the possible heart-protective effects of AP-AD PNPs made using the emulsification method. Compared to microfluidics, the emulsification method fabricated small-size nanoparticles, which are better at encapsulating drugs, retaining more drugs, and having a low viscosity for the myocardial infarction (MI) injection. TheMI model was developed using SD rats injected under the skin with 85 mg/kg doxorubicin (DOX) for 2 days. The metabolic results showed that our AP-AD PNPs accelerated the blood flow in rats with MI, which increased the amounts of AP and AD in the circulatory system. This led to significant improvements in the cardiac index and lower amounts of AST, LDH, and CK in the blood. A histopathological study using Hematoxylin&eosin, and TUNEL staining showed that cardiac function had improved and apoptosis had decreased. Moreover, tests that checked the amounts of IL-6, TNF-α, NO, GSH, MDA, and SOD showed that AP-AD PNPs may help treat MI by reducing oxidative stress and inflammation, making it a potentially useful therapeutic approach. |
| format | Article |
| id | doaj-art-e3a83ae6039e4778855fc53418516a39 |
| institution | DOAJ |
| issn | 1472-6750 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj-art-e3a83ae6039e4778855fc53418516a392025-08-20T02:50:08ZengBMCBMC Biotechnology1472-67502024-11-0124111410.1186/s12896-024-00912-yFabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stressRuixuan Li0Aixia Xu1Ye Chen2Yihui Li3Ru Fu4Weihong Jiang5Xiaogang Li6Department of Cardiology, The Third Xiangya Hospital of Central South UniversityDepartment of Endocrinology, Changsha Central HospitalDepartment of Cardiology, The Third Xiangya Hospital of Central South UniversityDepartment of Cardiology, The Third Xiangya Hospital of Central South UniversityDepartment of Cardiology, The Third Xiangya Hospital of Central South UniversityDepartment of Cardiology, The Third Xiangya Hospital of Central South UniversityDepartment of Cardiology, The Third Xiangya Hospital of Central South UniversityAbstract The study’s goals are to fabricate PLGA nanoparticles (PNPs) loaded with apigenin (AP) and adenosine (AD) using a microfluidic preparation method to a standard emulsification method and investigate the possible heart-protective effects of AP-AD PNPs made using the emulsification method. Compared to microfluidics, the emulsification method fabricated small-size nanoparticles, which are better at encapsulating drugs, retaining more drugs, and having a low viscosity for the myocardial infarction (MI) injection. TheMI model was developed using SD rats injected under the skin with 85 mg/kg doxorubicin (DOX) for 2 days. The metabolic results showed that our AP-AD PNPs accelerated the blood flow in rats with MI, which increased the amounts of AP and AD in the circulatory system. This led to significant improvements in the cardiac index and lower amounts of AST, LDH, and CK in the blood. A histopathological study using Hematoxylin&eosin, and TUNEL staining showed that cardiac function had improved and apoptosis had decreased. Moreover, tests that checked the amounts of IL-6, TNF-α, NO, GSH, MDA, and SOD showed that AP-AD PNPs may help treat MI by reducing oxidative stress and inflammation, making it a potentially useful therapeutic approach.https://doi.org/10.1186/s12896-024-00912-yMyocardial infarctionApigeninAdenosinePLGA nanoparticlesNanomedicineEmulsification method |
| spellingShingle | Ruixuan Li Aixia Xu Ye Chen Yihui Li Ru Fu Weihong Jiang Xiaogang Li Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress BMC Biotechnology Myocardial infarction Apigenin Adenosine PLGA nanoparticles Nanomedicine Emulsification method |
| title | Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress |
| title_full | Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress |
| title_fullStr | Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress |
| title_full_unstemmed | Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress |
| title_short | Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress |
| title_sort | fabrication of apigenin and adenosine loaded nanoparticles against doxorubicin induced myocardial infarction by reducing inflammation and oxidative stress |
| topic | Myocardial infarction Apigenin Adenosine PLGA nanoparticles Nanomedicine Emulsification method |
| url | https://doi.org/10.1186/s12896-024-00912-y |
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