Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation

Background/Aim. The vascular supply of dysplastic nodules (DN) is altered compared with surrounding cirrhotic nodules. Dysplastic nodules contain unpaired arteries which are isolated arteries unaccompained by bille ducts. In adition, capillarization or neovascularization is evident on CD34 and CD31...

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Main Authors: Gligorijević Jasmina, Đorđević Biljana, Petrović Aleksandar, Radičević Aleksandra, Stojanović Simonida
Format: Article
Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2010-01-01
Series:Vojnosanitetski Pregled
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501006459G.pdf
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author Gligorijević Jasmina
Đorđević Biljana
Petrović Aleksandar
Radičević Aleksandra
Stojanović Simonida
author_facet Gligorijević Jasmina
Đorđević Biljana
Petrović Aleksandar
Radičević Aleksandra
Stojanović Simonida
author_sort Gligorijević Jasmina
collection DOAJ
description Background/Aim. The vascular supply of dysplastic nodules (DN) is altered compared with surrounding cirrhotic nodules. Dysplastic nodules contain unpaired arteries which are isolated arteries unaccompained by bille ducts. In adition, capillarization or neovascularization is evident on CD34 and CD31 staining. The investigation of angiogenic profile of regenerative, dysplastic and nodules of hepatocellular carcinoma aimed at assessing whether vascular profile is in reliance to the process of dedifferentiation of hepatocytes during the course of cirrhosis. Methods. Thirty four liver nodules from surgical biopsies of 12 patients previously undiagnosed to have cirrhosis, were classified as regenerative, dysplastic and small hepatocellular carcinomas (HCC). The investigation included 8 large regenerative nodules (LRN), 11 low grade dysplastic nodules (LGDN), 12 high grade dysplastic nodules (HGDN) and 3 early HCC. Serial sections of the nodules and surrounding cirrhotic liver tissue were immunostained against CD34. The vascular counting method was performed. The results were analysed using SPSS computer statistical program. Results. The number of capillary unites showed significant differences among nodular types, with the largest number of capillaries in hepatocellular carcinoma as well as strong reliance to dedifferentiation. Conclusion. There is a significant correlation of sinusoidal capillarization to dediferentiation of the liver tissue during the course of cirrhosis. From diagnostic view, capillary counting may be helpfull to distinguish dysplastic from nondysplastic nodules. The appearance of dysplastic nodules in nonselected surgical biopsies is frequent enough to challenge caution during the follow-up of cirrhotic patients.
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spelling doaj-art-e3a7c47d82084d74a8cede596c41917e2025-08-20T03:25:07ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502010-01-0167645946210.2298/VSP1006459GExpression of CD34 in cirrhotic liver: Reliance to dedifferentiationGligorijević JasminaĐorđević BiljanaPetrović AleksandarRadičević AleksandraStojanović SimonidaBackground/Aim. The vascular supply of dysplastic nodules (DN) is altered compared with surrounding cirrhotic nodules. Dysplastic nodules contain unpaired arteries which are isolated arteries unaccompained by bille ducts. In adition, capillarization or neovascularization is evident on CD34 and CD31 staining. The investigation of angiogenic profile of regenerative, dysplastic and nodules of hepatocellular carcinoma aimed at assessing whether vascular profile is in reliance to the process of dedifferentiation of hepatocytes during the course of cirrhosis. Methods. Thirty four liver nodules from surgical biopsies of 12 patients previously undiagnosed to have cirrhosis, were classified as regenerative, dysplastic and small hepatocellular carcinomas (HCC). The investigation included 8 large regenerative nodules (LRN), 11 low grade dysplastic nodules (LGDN), 12 high grade dysplastic nodules (HGDN) and 3 early HCC. Serial sections of the nodules and surrounding cirrhotic liver tissue were immunostained against CD34. The vascular counting method was performed. The results were analysed using SPSS computer statistical program. Results. The number of capillary unites showed significant differences among nodular types, with the largest number of capillaries in hepatocellular carcinoma as well as strong reliance to dedifferentiation. Conclusion. There is a significant correlation of sinusoidal capillarization to dediferentiation of the liver tissue during the course of cirrhosis. From diagnostic view, capillary counting may be helpfull to distinguish dysplastic from nondysplastic nodules. The appearance of dysplastic nodules in nonselected surgical biopsies is frequent enough to challenge caution during the follow-up of cirrhotic patients.http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501006459G.pdfliver cirrhosiscarcinomahepatocellularantigenscd34diagnosiscell dedifferentiation
spellingShingle Gligorijević Jasmina
Đorđević Biljana
Petrović Aleksandar
Radičević Aleksandra
Stojanović Simonida
Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
Vojnosanitetski Pregled
liver cirrhosis
carcinoma
hepatocellular
antigens
cd34
diagnosis
cell dedifferentiation
title Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
title_full Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
title_fullStr Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
title_full_unstemmed Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
title_short Expression of CD34 in cirrhotic liver: Reliance to dedifferentiation
title_sort expression of cd34 in cirrhotic liver reliance to dedifferentiation
topic liver cirrhosis
carcinoma
hepatocellular
antigens
cd34
diagnosis
cell dedifferentiation
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501006459G.pdf
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AT radicevicaleksandra expressionofcd34incirrhoticliverreliancetodedifferentiation
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