Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan
The aim of the present research is the isolation and morphological and molecular–phenological identification of nematophagous fungi of Southern Kazakhstan for the production of effective bionematicides on their basis. Nematophagous fungi, which include nematode-trapping, ovicidal, endoparasitic, tox...
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2025-01-01
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| author | Gulzat Kanalbek Akniyet Zhanuzak Dmitry Faleev Aidos Nusupov Karlygash Mukhatayeva Kenzhe-Karim Boguspaev |
| author_facet | Gulzat Kanalbek Akniyet Zhanuzak Dmitry Faleev Aidos Nusupov Karlygash Mukhatayeva Kenzhe-Karim Boguspaev |
| author_sort | Gulzat Kanalbek |
| collection | DOAJ |
| description | The aim of the present research is the isolation and morphological and molecular–phenological identification of nematophagous fungi of Southern Kazakhstan for the production of effective bionematicides on their basis. Nematophagous fungi, which include nematode-trapping, ovicidal, endoparasitic, toxin-producing, and special substance-producing fungi, are among the most effective biological agents in controlling phytoparasitic nematodes. To isolate and characterize nematophagous fungi, soil samples were collected at 12 sites in three regions of Southern Kazakhstan. The samples were collected using the envelope method. The content of nematophagous fungi in the samples was determined using the standard surface sowing technique. The obtained strains of nematophagous fungi were identified. The attractive and nematophagous activity of the obtained fungal strains was determined by using standard methods. In experiments on the isolation and morphological identification of nematophagous fungi, the nematode species <i>Meloidogyne incognita</i> was used. Identification of the strains was carried out by the method of determining the direct nucleotide sequence of the region of the nuclear ribosomal internal transcribed spacer, followed by determination of nucleotide identity with sequences deposited in the international GeneBank database. As a result, the following species of nematophagous fungi living in the soils of agricultural lands in Southern Kazakhstan were identified: <i>Orbilia oligospora</i>, <i>Duddingtonia flagrans</i>, <i>Orbilia oligospora</i>, and <i>Arthrobotrys superba</i>. |
| format | Article |
| id | doaj-art-e3a79ef5a0774017b8eeab9c3904b28b |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-e3a79ef5a0774017b8eeab9c3904b28b2025-01-24T13:37:20ZengMDPI AGJournal of Fungi2309-608X2025-01-011114210.3390/jof11010042Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern KazakhstanGulzat Kanalbek0Akniyet Zhanuzak1Dmitry Faleev2Aidos Nusupov3Karlygash Mukhatayeva4Kenzhe-Karim Boguspaev5Faculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, KazakhstanFaculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, KazakhstanFaculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, KazakhstanFaculty of Technology, Almaty Technological University, Almaty 050012, KazakhstanFaculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, KazakhstanFaculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, KazakhstanThe aim of the present research is the isolation and morphological and molecular–phenological identification of nematophagous fungi of Southern Kazakhstan for the production of effective bionematicides on their basis. Nematophagous fungi, which include nematode-trapping, ovicidal, endoparasitic, toxin-producing, and special substance-producing fungi, are among the most effective biological agents in controlling phytoparasitic nematodes. To isolate and characterize nematophagous fungi, soil samples were collected at 12 sites in three regions of Southern Kazakhstan. The samples were collected using the envelope method. The content of nematophagous fungi in the samples was determined using the standard surface sowing technique. The obtained strains of nematophagous fungi were identified. The attractive and nematophagous activity of the obtained fungal strains was determined by using standard methods. In experiments on the isolation and morphological identification of nematophagous fungi, the nematode species <i>Meloidogyne incognita</i> was used. Identification of the strains was carried out by the method of determining the direct nucleotide sequence of the region of the nuclear ribosomal internal transcribed spacer, followed by determination of nucleotide identity with sequences deposited in the international GeneBank database. As a result, the following species of nematophagous fungi living in the soils of agricultural lands in Southern Kazakhstan were identified: <i>Orbilia oligospora</i>, <i>Duddingtonia flagrans</i>, <i>Orbilia oligospora</i>, and <i>Arthrobotrys superba</i>.https://www.mdpi.com/2309-608X/11/1/42soilnematodesnematophagous fungi (NF)phylogenynematicides |
| spellingShingle | Gulzat Kanalbek Akniyet Zhanuzak Dmitry Faleev Aidos Nusupov Karlygash Mukhatayeva Kenzhe-Karim Boguspaev Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan Journal of Fungi soil nematodes nematophagous fungi (NF) phylogeny nematicides |
| title | Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan |
| title_full | Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan |
| title_fullStr | Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan |
| title_full_unstemmed | Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan |
| title_short | Isolation, Morphological and Molecular–Phenological Identification of Nematophagous Fungi Inhabiting the Soils of Agricultural Lands in Southern Kazakhstan |
| title_sort | isolation morphological and molecular phenological identification of nematophagous fungi inhabiting the soils of agricultural lands in southern kazakhstan |
| topic | soil nematodes nematophagous fungi (NF) phylogeny nematicides |
| url | https://www.mdpi.com/2309-608X/11/1/42 |
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