Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique
Summary: Dermal fibroblasts play a central role in the production and remodeling of dermal extracellular matrix. Their spatially distinct subpopulations exhibit different functions during skin homeostasis, wound healing, and other diseases such as fibrosis and cancer. Here, we present a protocol for...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002072 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850186619113439232 |
|---|---|
| author | Toru Hiratsuka Emanuel Rognoni |
| author_facet | Toru Hiratsuka Emanuel Rognoni |
| author_sort | Toru Hiratsuka |
| collection | DOAJ |
| description | Summary: Dermal fibroblasts play a central role in the production and remodeling of dermal extracellular matrix. Their spatially distinct subpopulations exhibit different functions during skin homeostasis, wound healing, and other diseases such as fibrosis and cancer. Here, we present a protocol for detecting 3D dermal fibroblast cellular dynamics in mice without fixing the tissue using an intravital imaging technique. We describe steps for preparing, labeling, and mounting mice. We then describe detailed procedures for intravital imaging, recovery, and quantification.For complete details on the use and execution of this protocol, please refer to Rognoni et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-e399f711b6ee45a4af8e7e780de0a453 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-e399f711b6ee45a4af8e7e780de0a4532025-08-20T02:16:18ZengElsevierSTAR Protocols2666-16672025-06-016210380110.1016/j.xpro.2025.103801Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging techniqueToru Hiratsuka0Emanuel Rognoni1Department of Oncogenesis and Growth Regulation, Research Center, Osaka International Cancer Institute, Chuoku, Osaka 541-8567, Japan; Department of Molcular Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; Corresponding authorCentre for Cell Biology and Cutaneous Research, The Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, UKSummary: Dermal fibroblasts play a central role in the production and remodeling of dermal extracellular matrix. Their spatially distinct subpopulations exhibit different functions during skin homeostasis, wound healing, and other diseases such as fibrosis and cancer. Here, we present a protocol for detecting 3D dermal fibroblast cellular dynamics in mice without fixing the tissue using an intravital imaging technique. We describe steps for preparing, labeling, and mounting mice. We then describe detailed procedures for intravital imaging, recovery, and quantification.For complete details on the use and execution of this protocol, please refer to Rognoni et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002072Single CellMicroscopyModel Organisms |
| spellingShingle | Toru Hiratsuka Emanuel Rognoni Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique STAR Protocols Single Cell Microscopy Model Organisms |
| title | Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| title_full | Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| title_fullStr | Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| title_full_unstemmed | Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| title_short | Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| title_sort | protocol for detecting 3d dermal fibroblast cellular dynamics in mice using an intravital imaging technique |
| topic | Single Cell Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002072 |
| work_keys_str_mv | AT toruhiratsuka protocolfordetecting3ddermalfibroblastcellulardynamicsinmiceusinganintravitalimagingtechnique AT emanuelrognoni protocolfordetecting3ddermalfibroblastcellulardynamicsinmiceusinganintravitalimagingtechnique |