Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC

Abstract Due to the disruption in the supply of consumables for platelet separation on the COBE Spectra blood component separator in China, we have performed therapeutic thrombocytapheresis using its mononuclear cell collection procedure (MNC procedure) and compared it with the therapy by COM.TEC pl...

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Main Authors: Yong Wu, Yuanjun Wu, Huisen Li, Siyuan Liang, Dongsheng Wu, Baochan Chen, Yan Liu, Yueqin Wu, Qian Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-05282-9
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author Yong Wu
Yuanjun Wu
Huisen Li
Siyuan Liang
Dongsheng Wu
Baochan Chen
Yan Liu
Yueqin Wu
Qian Wang
author_facet Yong Wu
Yuanjun Wu
Huisen Li
Siyuan Liang
Dongsheng Wu
Baochan Chen
Yan Liu
Yueqin Wu
Qian Wang
author_sort Yong Wu
collection DOAJ
description Abstract Due to the disruption in the supply of consumables for platelet separation on the COBE Spectra blood component separator in China, we have performed therapeutic thrombocytapheresis using its mononuclear cell collection procedure (MNC procedure) and compared it with the therapy by COM.TEC platelet donation procedure to compare the influence on patient blood cell indicators. Therapeutic thrombocytapheresis was conducted 69 and 35 times on 38 and 15 clonal thrombocytosis patients using the MNC procedure with the COBE Spectra (MNC group) and the PLT-5d C5L procedure of the COM.TEC platelet donation package (PLT-5d C5L group), respectively. In the MNC and PLT-5d C5L groups, the therapy durations and the processed blood volumes were similar: platelet removal rates, 60.56% (53.59–75.81%) and (84.95 ± 39.46)% (z = 3.017, p < 0.01); white blood cell (WBC) loss values, 2.85 (2.02–3.83) × 109/L and 0.69 (0.23–1.47) × 109/L (z = 8.307, p < 0.001); and haemoglobin (Hb) loss values, 0.75 (0.50–1.09) g/L and 0.03 (0.02–0.20) g/L (z = 8.311, p < 0.001) in the MNC and PLT-5d C5L groups, respectively. However, the WBC count decreased by 1.28 (− 0.84 to 2.49) × 109/L and (2.97 ± 3.49) × 109/L (z = 2.825, p < 0.01), and the Hb level decreased by (3.38 ± 9.36) g/L and (9.06 ± 7.05) g/L (t = − 3.158, p < 0.01) in the MNC and PLT-5d C5L groups, respectively. The results show that, compared with the COBE Spectra MNC procedure, although the COM.TEC platelet donation package PLT-5d C5L procedure demonstrated higher therapeutic thrombocytapheresis efficiency, it also resulted in a greater decrease in WBCs and Hb.
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spelling doaj-art-e3938578929c499a8fc511ad531af0c32025-08-20T03:37:20ZengNature PortfolioScientific Reports2045-23222025-07-011511610.1038/s41598-025-05282-9Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TECYong Wu0Yuanjun Wu1Huisen Li2Siyuan Liang3Dongsheng Wu4Baochan Chen5Yan Liu6Yueqin Wu7Qian Wang8Department of Transfusion Medicine, Dongguan Tungwah HospitalDepartment of Transfusion Medicine, Dongguan Maternal and Child Health Care HospitalDepartment of Transfusion Medicine, Dongguan Tungwah HospitalDepartment of Transfusion Medicine, Dongguan Tungwah HospitalDepartment of Hematology, Dongguan Tungwah HospitalDepartment of Transfusion Medicine, Dongguan Tungwah HospitalDepartment of Transfusion Medicine, Dongguan Tungwah HospitalDepartment of Transfusion Medicine, Dongguan Songshan Lake Tungwah HospitalDepartment of Hematology, Dongguan Tungwah HospitalAbstract Due to the disruption in the supply of consumables for platelet separation on the COBE Spectra blood component separator in China, we have performed therapeutic thrombocytapheresis using its mononuclear cell collection procedure (MNC procedure) and compared it with the therapy by COM.TEC platelet donation procedure to compare the influence on patient blood cell indicators. Therapeutic thrombocytapheresis was conducted 69 and 35 times on 38 and 15 clonal thrombocytosis patients using the MNC procedure with the COBE Spectra (MNC group) and the PLT-5d C5L procedure of the COM.TEC platelet donation package (PLT-5d C5L group), respectively. In the MNC and PLT-5d C5L groups, the therapy durations and the processed blood volumes were similar: platelet removal rates, 60.56% (53.59–75.81%) and (84.95 ± 39.46)% (z = 3.017, p < 0.01); white blood cell (WBC) loss values, 2.85 (2.02–3.83) × 109/L and 0.69 (0.23–1.47) × 109/L (z = 8.307, p < 0.001); and haemoglobin (Hb) loss values, 0.75 (0.50–1.09) g/L and 0.03 (0.02–0.20) g/L (z = 8.311, p < 0.001) in the MNC and PLT-5d C5L groups, respectively. However, the WBC count decreased by 1.28 (− 0.84 to 2.49) × 109/L and (2.97 ± 3.49) × 109/L (z = 2.825, p < 0.01), and the Hb level decreased by (3.38 ± 9.36) g/L and (9.06 ± 7.05) g/L (t = − 3.158, p < 0.01) in the MNC and PLT-5d C5L groups, respectively. The results show that, compared with the COBE Spectra MNC procedure, although the COM.TEC platelet donation package PLT-5d C5L procedure demonstrated higher therapeutic thrombocytapheresis efficiency, it also resulted in a greater decrease in WBCs and Hb.https://doi.org/10.1038/s41598-025-05282-9PlateletsThrombocytosisTherapeutic thrombocytapheresisWhite blood cellsHemoglobin
spellingShingle Yong Wu
Yuanjun Wu
Huisen Li
Siyuan Liang
Dongsheng Wu
Baochan Chen
Yan Liu
Yueqin Wu
Qian Wang
Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
Scientific Reports
Platelets
Thrombocytosis
Therapeutic thrombocytapheresis
White blood cells
Hemoglobin
title Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
title_full Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
title_fullStr Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
title_full_unstemmed Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
title_short Therapeutic thrombocytapheresis short-term efficacy and hematologic impact: comparing MNC procedure of COBE spectra and PLT-5d C5L procedure of COM.TEC
title_sort therapeutic thrombocytapheresis short term efficacy and hematologic impact comparing mnc procedure of cobe spectra and plt 5d c5l procedure of com tec
topic Platelets
Thrombocytosis
Therapeutic thrombocytapheresis
White blood cells
Hemoglobin
url https://doi.org/10.1038/s41598-025-05282-9
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