mRNA mediates passive vaccination against infectious agents, toxins, and tumors
Abstract The delivery of genetic information has emerged as a valid therapeutic approach. Various reports have demonstrated that mRNA, besides its remarkable potential as vaccine, can also promote expression without inducing an adverse immune response against the encoded protein. In the current stud...
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| Format: | Article |
| Language: | English |
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Springer Nature
2017-08-01
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| Series: | EMBO Molecular Medicine |
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| Online Access: | https://doi.org/10.15252/emmm.201707678 |
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| author | Moritz Thran Jean Mukherjee Marion Pönisch Katja Fiedler Andreas Thess Barbara L Mui Michael J Hope Ying K Tam Nigel Horscroft Regina Heidenreich Mariola Fotin‐Mleczek Charles B Shoemaker Thomas Schlake |
| author_facet | Moritz Thran Jean Mukherjee Marion Pönisch Katja Fiedler Andreas Thess Barbara L Mui Michael J Hope Ying K Tam Nigel Horscroft Regina Heidenreich Mariola Fotin‐Mleczek Charles B Shoemaker Thomas Schlake |
| author_sort | Moritz Thran |
| collection | DOAJ |
| description | Abstract The delivery of genetic information has emerged as a valid therapeutic approach. Various reports have demonstrated that mRNA, besides its remarkable potential as vaccine, can also promote expression without inducing an adverse immune response against the encoded protein. In the current study, we set out to explore whether our technology based on chemically unmodified mRNA is suitable for passive immunization. To this end, various antibodies using different designs were expressed and characterized in vitro and in vivo in the fields of viral infections, toxin exposure, and cancer immunotherapies. Single injections of mRNA–lipid nanoparticle (LNP) were sufficient to establish rapid, strong, and long‐lasting serum antibody titers in vivo, thereby enabling both prophylactic and therapeutic protection against lethal rabies infection or botulinum intoxication. Moreover, therapeutic mRNA‐mediated antibody expression allowed mice to survive an otherwise lethal tumor challenge. In conclusion, the present study demonstrates the utility of formulated mRNA as a potent novel technology for passive immunization. |
| format | Article |
| id | doaj-art-e38e62606e0c43b694eb441946af61b3 |
| institution | Kabale University |
| issn | 1757-4676 1757-4684 |
| language | English |
| publishDate | 2017-08-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | EMBO Molecular Medicine |
| spelling | doaj-art-e38e62606e0c43b694eb441946af61b32025-08-20T04:03:07ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842017-08-019101434144710.15252/emmm.201707678mRNA mediates passive vaccination against infectious agents, toxins, and tumorsMoritz Thran0Jean Mukherjee1Marion Pönisch2Katja Fiedler3Andreas Thess4Barbara L Mui5Michael J Hope6Ying K Tam7Nigel Horscroft8Regina Heidenreich9Mariola Fotin‐Mleczek10Charles B Shoemaker11Thomas Schlake12CureVac AGDepartment of Infectious Disease and Global Health, Tufts Cummings School of Veterinary MedicineCureVac AGCureVac AGCureVac AGAcuitas TherapeuticsAcuitas TherapeuticsAcuitas TherapeuticsCureVac AGCureVac AGCureVac AGDepartment of Infectious Disease and Global Health, Tufts Cummings School of Veterinary MedicineCureVac AGAbstract The delivery of genetic information has emerged as a valid therapeutic approach. Various reports have demonstrated that mRNA, besides its remarkable potential as vaccine, can also promote expression without inducing an adverse immune response against the encoded protein. In the current study, we set out to explore whether our technology based on chemically unmodified mRNA is suitable for passive immunization. To this end, various antibodies using different designs were expressed and characterized in vitro and in vivo in the fields of viral infections, toxin exposure, and cancer immunotherapies. Single injections of mRNA–lipid nanoparticle (LNP) were sufficient to establish rapid, strong, and long‐lasting serum antibody titers in vivo, thereby enabling both prophylactic and therapeutic protection against lethal rabies infection or botulinum intoxication. Moreover, therapeutic mRNA‐mediated antibody expression allowed mice to survive an otherwise lethal tumor challenge. In conclusion, the present study demonstrates the utility of formulated mRNA as a potent novel technology for passive immunization.https://doi.org/10.15252/emmm.201707678antibodylipid nanoparticlemessenger RNAmousepassive immunization |
| spellingShingle | Moritz Thran Jean Mukherjee Marion Pönisch Katja Fiedler Andreas Thess Barbara L Mui Michael J Hope Ying K Tam Nigel Horscroft Regina Heidenreich Mariola Fotin‐Mleczek Charles B Shoemaker Thomas Schlake mRNA mediates passive vaccination against infectious agents, toxins, and tumors EMBO Molecular Medicine antibody lipid nanoparticle messenger RNA mouse passive immunization |
| title | mRNA mediates passive vaccination against infectious agents, toxins, and tumors |
| title_full | mRNA mediates passive vaccination against infectious agents, toxins, and tumors |
| title_fullStr | mRNA mediates passive vaccination against infectious agents, toxins, and tumors |
| title_full_unstemmed | mRNA mediates passive vaccination against infectious agents, toxins, and tumors |
| title_short | mRNA mediates passive vaccination against infectious agents, toxins, and tumors |
| title_sort | mrna mediates passive vaccination against infectious agents toxins and tumors |
| topic | antibody lipid nanoparticle messenger RNA mouse passive immunization |
| url | https://doi.org/10.15252/emmm.201707678 |
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