Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics
Summary: The intricate development and functionality of the mammalian heart are influenced by the heterogeneous nature of cardiomyocytes (CMs). In this study, single-cell and spatial transcriptomics were utilized to analyze cells from neonatal mouse hearts, resulting in a comprehensive atlas delinea...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224028232 |
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| author | Xiaoying Wang Lizhi Cao Rui Chang Junwei Shen Linlin Ma Yanfei Li |
| author_facet | Xiaoying Wang Lizhi Cao Rui Chang Junwei Shen Linlin Ma Yanfei Li |
| author_sort | Xiaoying Wang |
| collection | DOAJ |
| description | Summary: The intricate development and functionality of the mammalian heart are influenced by the heterogeneous nature of cardiomyocytes (CMs). In this study, single-cell and spatial transcriptomics were utilized to analyze cells from neonatal mouse hearts, resulting in a comprehensive atlas delineating the transcriptional profiles of distinct CM subsets. A continuum of maturation states was elucidated, emphasizing a progressive developmental trajectory rather than discrete stages. This approach enabled the mapping of these states across various cardiac regions, illuminating the spatial organization of CM development and the influence of the cellular microenvironment. Notably, a subset of transitional CMs was identified, characterized by a transcriptional signature marking a pivotal maturation phase, presenting a promising target for therapeutic strategies aimed at enhancing cardiac regeneration. This atlas not only elucidates fundamental aspects of cardiac development but also serves as a valuable resource for advancing research into cardiac physiology and pathology, with significant implications for regenerative medicine. |
| format | Article |
| id | doaj-art-e378e555f485499993d7f8764c2ab47a |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-e378e555f485499993d7f8764c2ab47a2025-08-20T01:57:20ZengElsevieriScience2589-00422025-01-0128111159610.1016/j.isci.2024.111596Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomicsXiaoying Wang0Lizhi Cao1Rui Chang2Junwei Shen3Linlin Ma4Yanfei Li5Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; School of Life Sciences and Technology, Tongji University, Shanghai, China; Institute of Biophysics, Chinese Academy of Sciences, Beijing, ChinaShanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, ChinaSchool of Life Sciences and Technology, Tongji University, Shanghai, ChinaShanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; Corresponding authorShanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China; Corresponding authorSummary: The intricate development and functionality of the mammalian heart are influenced by the heterogeneous nature of cardiomyocytes (CMs). In this study, single-cell and spatial transcriptomics were utilized to analyze cells from neonatal mouse hearts, resulting in a comprehensive atlas delineating the transcriptional profiles of distinct CM subsets. A continuum of maturation states was elucidated, emphasizing a progressive developmental trajectory rather than discrete stages. This approach enabled the mapping of these states across various cardiac regions, illuminating the spatial organization of CM development and the influence of the cellular microenvironment. Notably, a subset of transitional CMs was identified, characterized by a transcriptional signature marking a pivotal maturation phase, presenting a promising target for therapeutic strategies aimed at enhancing cardiac regeneration. This atlas not only elucidates fundamental aspects of cardiac development but also serves as a valuable resource for advancing research into cardiac physiology and pathology, with significant implications for regenerative medicine.http://www.sciencedirect.com/science/article/pii/S2589004224028232Cell biologyOmicsTranscriptomics |
| spellingShingle | Xiaoying Wang Lizhi Cao Rui Chang Junwei Shen Linlin Ma Yanfei Li Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics iScience Cell biology Omics Transcriptomics |
| title | Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics |
| title_full | Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics |
| title_fullStr | Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics |
| title_full_unstemmed | Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics |
| title_short | Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics |
| title_sort | elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single cell and spatial transcriptomics |
| topic | Cell biology Omics Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224028232 |
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