Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes
(1) Background: Knee cartilage injury is at the top of the rising concerns among bone and joint disorder patients. Autologous chondrocyte implantation (ACI) is widely used to approach knee cartilage deterioration. Integrating autologous chondrocytes and periosteal patches aids in forming new cartila...
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MDPI AG
2025-03-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/3/92 |
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| author | Phuong-Vy Bui Vang Pham Thi Trung-Nhan Vo Viet-Trinh Nguyen Thai-Duong Tran Vy-Khanh Vo Phuong Le Thi Dieu Linh Tran Minh-Dung Truong |
| author_facet | Phuong-Vy Bui Vang Pham Thi Trung-Nhan Vo Viet-Trinh Nguyen Thai-Duong Tran Vy-Khanh Vo Phuong Le Thi Dieu Linh Tran Minh-Dung Truong |
| author_sort | Phuong-Vy Bui |
| collection | DOAJ |
| description | (1) Background: Knee cartilage injury is at the top of the rising concerns among bone and joint disorder patients. Autologous chondrocyte implantation (ACI) is widely used to approach knee cartilage deterioration. Integrating autologous chondrocytes and periosteal patches aids in forming new cartilage-like tissue at the lesion area. This study uses a novel cell source from one-day-old porcine cartilage to fabricate a biomembrane as a substitute for periosteal membranes in cell implantation techniques for treating knee cartilage injuries. (2) Methods: Cells isolated from one-day-old porcine cartilage tissue were identified and assessed for their proliferation capability, differentiation ability, and membrane formation potential. The protein component of the biomembrane was also defined by proteomics. The cartilage repair ability was also confirmed using an in vitro transplantation model. (3) Results: Negative results for porcine infectious diseases are pivotal in selecting suitable piglets to provide cartilage tissue. The cells successfully obtained from one-day-old porcine cartilage exhibited stem-cell-like characteristics (CD34-, CD45-, CD90+, CD105+), including a high proliferation to 20 passages (doubling time: 1–2 days) and a capacity to differentiate into various cell types (osteogenesis, adipogenesis, and chondrogenesis). The stem cells were successfully applied in the fabrication of the biomembranes. The protein components of the biomembrane included an extracellular matrix and growth factors. The in vitro transplantation model showed that the biomembrane induced the repair ability of cartilage defects. (4) Conclusions: This study is the first to successfully harvest stem cells from one-day-old porcine cartilage for biomembrane fabrication for a knee cartilage injury therapeutic application. |
| format | Article |
| id | doaj-art-e3719ab154264eb0a98a754fdce66dd1 |
| institution | OA Journals |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-e3719ab154264eb0a98a754fdce66dd12025-08-20T02:11:05ZengMDPI AGJournal of Functional Biomaterials2079-49832025-03-011639210.3390/jfb16030092Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell BiomembranesPhuong-Vy Bui0Vang Pham Thi1Trung-Nhan Vo2Viet-Trinh Nguyen3Thai-Duong Tran4Vy-Khanh Vo5Phuong Le Thi6Dieu Linh Tran7Minh-Dung Truong8Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, VietnamInstitute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, VietnamInstitute of Chemical Technology, Vietnam Academy of Science and Technology, 1A TL29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City 700000, VietnamBiotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam(1) Background: Knee cartilage injury is at the top of the rising concerns among bone and joint disorder patients. Autologous chondrocyte implantation (ACI) is widely used to approach knee cartilage deterioration. Integrating autologous chondrocytes and periosteal patches aids in forming new cartilage-like tissue at the lesion area. This study uses a novel cell source from one-day-old porcine cartilage to fabricate a biomembrane as a substitute for periosteal membranes in cell implantation techniques for treating knee cartilage injuries. (2) Methods: Cells isolated from one-day-old porcine cartilage tissue were identified and assessed for their proliferation capability, differentiation ability, and membrane formation potential. The protein component of the biomembrane was also defined by proteomics. The cartilage repair ability was also confirmed using an in vitro transplantation model. (3) Results: Negative results for porcine infectious diseases are pivotal in selecting suitable piglets to provide cartilage tissue. The cells successfully obtained from one-day-old porcine cartilage exhibited stem-cell-like characteristics (CD34-, CD45-, CD90+, CD105+), including a high proliferation to 20 passages (doubling time: 1–2 days) and a capacity to differentiate into various cell types (osteogenesis, adipogenesis, and chondrogenesis). The stem cells were successfully applied in the fabrication of the biomembranes. The protein components of the biomembrane included an extracellular matrix and growth factors. The in vitro transplantation model showed that the biomembrane induced the repair ability of cartilage defects. (4) Conclusions: This study is the first to successfully harvest stem cells from one-day-old porcine cartilage for biomembrane fabrication for a knee cartilage injury therapeutic application.https://www.mdpi.com/2079-4983/16/3/92autologous chondrocyte implantationbiomembraneone-day-old porcine cartilage |
| spellingShingle | Phuong-Vy Bui Vang Pham Thi Trung-Nhan Vo Viet-Trinh Nguyen Thai-Duong Tran Vy-Khanh Vo Phuong Le Thi Dieu Linh Tran Minh-Dung Truong Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes Journal of Functional Biomaterials autologous chondrocyte implantation biomembrane one-day-old porcine cartilage |
| title | Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes |
| title_full | Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes |
| title_fullStr | Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes |
| title_full_unstemmed | Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes |
| title_short | Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes |
| title_sort | fabrication and characterization of immature porcine cartilage derived cell biomembranes |
| topic | autologous chondrocyte implantation biomembrane one-day-old porcine cartilage |
| url | https://www.mdpi.com/2079-4983/16/3/92 |
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