Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells
Objective: Investigating the antitumor activity of rose-orange essential oil against colorectal cancer cells (HCT116). Methods: Ultrasound-assisted steam distillation was used to examine the effects of sodium chloride concentration, distillation time, liquid-to-material ratio, and ultrasound duratio...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2025-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090034 |
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| author | Yanjiao LIU Yajun ZHANG Jie CHEN Guosheng XIAO Qinmin ZHU Huihui DU Donglin YANG |
| author_facet | Yanjiao LIU Yajun ZHANG Jie CHEN Guosheng XIAO Qinmin ZHU Huihui DU Donglin YANG |
| author_sort | Yanjiao LIU |
| collection | DOAJ |
| description | Objective: Investigating the antitumor activity of rose-orange essential oil against colorectal cancer cells (HCT116). Methods: Ultrasound-assisted steam distillation was used to examine the effects of sodium chloride concentration, distillation time, liquid-to-material ratio, and ultrasound duration on the extraction of rose-orange essential oil. Extraction yield served as the optimization criterion, and the extraction process was optimized using a combination of single-factor experiments and response surface methodology. The in vitro anticancer activity of the essential oil was assessed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. The potential mechanism underlying its inhibitory effect on colorectal cancer cell proliferation was analyzed through flow cytometry, western blotting (WB), and immunofluorescence assays. Results: The optimal extraction conditions for rose-orange essential oil were a sodium chloride concentration of 5.6%, a distillation time of 117 minutes, a liquid-to-material ratio of 12:1 mL/g, and an ultrasound duration of 33 minutes, yielding an extraction rate of 3.53%. The essential oil inhibited the growth of HCT116 cells in a dose- and time-dependent manner, with an IC50 of 933.5 μg/mL. Mechanistically, rose-orange essential oil was found to down-regulate the expression of cyclin-dependent kinase 1 (CDK1) and cyclin B1, while simultaneously inhibiting the phosphorylation of serine/threonine protein kinase Aurora A, Aurora B/C, and histone H3. These effects collectively induced G2/M phase cell cycle arrest, resulting in suppressed proliferation of HCT116 cells. This study establishes a theoretical foundation for both the extraction process and antitumor efficacy of rose-orange essential oil, thereby supporting its further development for potential therapeutic applications. |
| format | Article |
| id | doaj-art-e36b4fe40eb8435180577cb589ca3b5a |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-e36b4fe40eb8435180577cb589ca3b5a2025-08-20T03:43:55ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-01461639640610.13386/j.issn1002-0306.20240900342024090034-16Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer CellsYanjiao LIU0Yajun ZHANG1Jie CHEN2Guosheng XIAO3Qinmin ZHU4Huihui DU5Donglin YANG6College of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, ChinaCollege of Pharmacy (International Academy of Targeted Therapeutics and Innovation), Chongqing University of Arts and Sciences, Chongqing 402160, ChinaCollege of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, ChinaCollege of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, ChinaCollege of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, ChinaCollege of Biological and Food Engineering, Chongqing Three Gorges University, Chongqing 404120, ChinaCollege of Pharmacy (International Academy of Targeted Therapeutics and Innovation), Chongqing University of Arts and Sciences, Chongqing 402160, ChinaObjective: Investigating the antitumor activity of rose-orange essential oil against colorectal cancer cells (HCT116). Methods: Ultrasound-assisted steam distillation was used to examine the effects of sodium chloride concentration, distillation time, liquid-to-material ratio, and ultrasound duration on the extraction of rose-orange essential oil. Extraction yield served as the optimization criterion, and the extraction process was optimized using a combination of single-factor experiments and response surface methodology. The in vitro anticancer activity of the essential oil was assessed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. The potential mechanism underlying its inhibitory effect on colorectal cancer cell proliferation was analyzed through flow cytometry, western blotting (WB), and immunofluorescence assays. Results: The optimal extraction conditions for rose-orange essential oil were a sodium chloride concentration of 5.6%, a distillation time of 117 minutes, a liquid-to-material ratio of 12:1 mL/g, and an ultrasound duration of 33 minutes, yielding an extraction rate of 3.53%. The essential oil inhibited the growth of HCT116 cells in a dose- and time-dependent manner, with an IC50 of 933.5 μg/mL. Mechanistically, rose-orange essential oil was found to down-regulate the expression of cyclin-dependent kinase 1 (CDK1) and cyclin B1, while simultaneously inhibiting the phosphorylation of serine/threonine protein kinase Aurora A, Aurora B/C, and histone H3. These effects collectively induced G2/M phase cell cycle arrest, resulting in suppressed proliferation of HCT116 cells. This study establishes a theoretical foundation for both the extraction process and antitumor efficacy of rose-orange essential oil, thereby supporting its further development for potential therapeutic applications.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090034rose-orange essential oilextraction processcolorectal cancerantitumor activity |
| spellingShingle | Yanjiao LIU Yajun ZHANG Jie CHEN Guosheng XIAO Qinmin ZHU Huihui DU Donglin YANG Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells Shipin gongye ke-ji rose-orange essential oil extraction process colorectal cancer antitumor activity |
| title | Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells |
| title_full | Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells |
| title_fullStr | Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells |
| title_full_unstemmed | Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells |
| title_short | Optimization of Extraction Process of Rose-orange Essential Oil and Analysis of Its Inhibitory Activity Against HCT116 Colorectal Cancer Cells |
| title_sort | optimization of extraction process of rose orange essential oil and analysis of its inhibitory activity against hct116 colorectal cancer cells |
| topic | rose-orange essential oil extraction process colorectal cancer antitumor activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090034 |
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