Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients
Background: Variable progression towards AIDS has been described and has been related to viral and host factors. Around 10% of the HIV-1 infected patients are slow progressors (SP), not presenting with AIDS disease signs even after more than 10 years of infection. Viral gene defects have been associ...
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The Journal of Infection in Developing Countries
2009-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/471 |
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| author | Héctor Rafael Rangel Domingo Garzaro Anny Karely Rodríguez Alvaro Hernán Ramírez Gladys Ameli Cristina del Rosario Gutiérrez Flor Helene Pujol |
| author_facet | Héctor Rafael Rangel Domingo Garzaro Anny Karely Rodríguez Alvaro Hernán Ramírez Gladys Ameli Cristina del Rosario Gutiérrez Flor Helene Pujol |
| author_sort | Héctor Rafael Rangel |
| collection | DOAJ |
| description | Background: Variable progression towards AIDS has been described and has been related to viral and host factors. Around 10% of the HIV-1 infected patients are slow progressors (SP), not presenting with AIDS disease signs even after more than 10 years of infection. Viral gene defects have been associated with the disease progression but more studies are still needed.
Methodology: The sequence of vif and nef were analyzed for HIV-1 infecting 14 SP and 46 normal progressors (NP) patients.
Results: Co-circulation of a strain carrying vif deleted gene with the wild type strain was detected in an SP patient with more than 10 years of infection. Other mutations (insertion in aa 63 in one strain, two premature stop codons in another one) were found in viruses infecting two other patients. Except for the SP8 strain, which exhibited a premature stop codon in nef, no gross deletions or insertions were observed in nef genes of both NPs and SPs strains analyzed.
Conclusions: Different kind of mutation: deletion, insertion and stop codon, were detected in 3/14 samples from SP, with co-circulation of a 195 bp vif deletion virus with a wild type in one of these patients. Although vif defects do not seem to be a frequent feature in SPs, this study illustrates the importance of analysing this gene, in addition to the multiple factors associated with the long-term non progression to AIDS. |
| format | Article |
| id | doaj-art-e36742bcd17d46fc820179a82579a6e1 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2009-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e36742bcd17d46fc820179a82579a6e12025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-08-0130710.3855/jidc.471Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patientsHéctor Rafael Rangel0Domingo Garzaro1Anny Karely Rodríguez2Alvaro Hernán Ramírez3Gladys Ameli4Cristina del Rosario Gutiérrez5Flor Helene Pujol6Laboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Apdo. 20632, Caracas 1020-ALaboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Apdo. 20632, Caracas 1020-ALaboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Apdo. 20632, Caracas 1020-ALaboratorio de Biología de Virus, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Apdo. 20632, Caracas 1020-AInstituto Nacional de Higiene Rafael Rangel, CaracasInstituto Nacional de Higiene Rafael Rangel, CaracasLaboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Apdo. 20632, Caracas 1020-ABackground: Variable progression towards AIDS has been described and has been related to viral and host factors. Around 10% of the HIV-1 infected patients are slow progressors (SP), not presenting with AIDS disease signs even after more than 10 years of infection. Viral gene defects have been associated with the disease progression but more studies are still needed. Methodology: The sequence of vif and nef were analyzed for HIV-1 infecting 14 SP and 46 normal progressors (NP) patients. Results: Co-circulation of a strain carrying vif deleted gene with the wild type strain was detected in an SP patient with more than 10 years of infection. Other mutations (insertion in aa 63 in one strain, two premature stop codons in another one) were found in viruses infecting two other patients. Except for the SP8 strain, which exhibited a premature stop codon in nef, no gross deletions or insertions were observed in nef genes of both NPs and SPs strains analyzed. Conclusions: Different kind of mutation: deletion, insertion and stop codon, were detected in 3/14 samples from SP, with co-circulation of a 195 bp vif deletion virus with a wild type in one of these patients. Although vif defects do not seem to be a frequent feature in SPs, this study illustrates the importance of analysing this gene, in addition to the multiple factors associated with the long-term non progression to AIDS.https://jidc.org/index.php/journal/article/view/471HIVAPOBEClong term progressorcontroller |
| spellingShingle | Héctor Rafael Rangel Domingo Garzaro Anny Karely Rodríguez Alvaro Hernán Ramírez Gladys Ameli Cristina del Rosario Gutiérrez Flor Helene Pujol Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients Journal of Infection in Developing Countries HIV APOBEC long term progressor controller |
| title | Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients |
| title_full | Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients |
| title_fullStr | Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients |
| title_full_unstemmed | Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients |
| title_short | Deletion, insertion and stop codon mutations in vif genes of HIV-1 infecting slow progressor patients |
| title_sort | deletion insertion and stop codon mutations in vif genes of hiv 1 infecting slow progressor patients |
| topic | HIV APOBEC long term progressor controller |
| url | https://jidc.org/index.php/journal/article/view/471 |
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