AMDA 2.13: A major update for automated cross-platform microarray data analysis

Microarray platforms require analytical pipelines with modules for data pre-processing including data normalization, statistical analysis for identification of differentially expressed genes, cluster analysis, and functional annotation. We previously developed the Automated Microarray Data Analysis...

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Main Authors: Dimos Kapetis, Ferdinando Clarelli, Federico Vitulli, Nicole Kerlero de Rosbo, Ottavio Beretta, Maria Foti, Paola Ricciardi-Castagnoli, Francesca Zolezzi
Format: Article
Language:English
Published: Taylor & Francis Group 2012-07-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/0000113889
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author Dimos Kapetis
Ferdinando Clarelli
Federico Vitulli
Nicole Kerlero de Rosbo
Ottavio Beretta
Maria Foti
Paola Ricciardi-Castagnoli
Francesca Zolezzi
author_facet Dimos Kapetis
Ferdinando Clarelli
Federico Vitulli
Nicole Kerlero de Rosbo
Ottavio Beretta
Maria Foti
Paola Ricciardi-Castagnoli
Francesca Zolezzi
author_sort Dimos Kapetis
collection DOAJ
description Microarray platforms require analytical pipelines with modules for data pre-processing including data normalization, statistical analysis for identification of differentially expressed genes, cluster analysis, and functional annotation. We previously developed the Automated Microarray Data Analysis (AMDA, version 2.3.5) pipeline to process Affymetrix 3′ IVT GeneChips. The availability of newer technologies that demand open-source tools for microarray data analysis has impelled us to develop an updated multi-platform version, AMDA 2.13. It includes additional quality control metrics, annotation-driven (annotation grade of Affymetrix NetAffx) and signal-driven (Inter-Quartile Range) gene filtering, and approaches to experimental design. To enhance understanding of biological data, differentially expressed genes have been mapped into KEGG pathways. Finally, a more stable and user-friendly interface was designed to integrate the requirements for different platforms. AMDA 2.13 allows the analysis of Affymetrix (cartridges and plates) and whole transcript probe design (Gene 1.0/1.1 ST and Exon 1.0 ST GeneChips), Illumina Bead Arrays, and one-channel Agilent 4×44 arrays. Relative to early versions, it supports various experimental designs and delivers more insightful biological understanding and up-to-date annotations.
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spelling doaj-art-e3644f933d8f4299871047cd7a1dd6282025-08-20T02:25:58ZengTaylor & Francis GroupBioTechniques0736-62051940-98182012-07-01531334010.2144/0000113889AMDA 2.13: A major update for automated cross-platform microarray data analysisDimos Kapetis0Ferdinando Clarelli1Federico Vitulli2Nicole Kerlero de Rosbo3Ottavio Beretta4Maria Foti5Paola Ricciardi-Castagnoli6Francesca Zolezzi71Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy2Fondazione IRCCS Istituto Neurologico Carlo Besta, Via Celoria 11, Milan, 20133 , Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, Italy1Genopolis-Consortium for Functional Genomics, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 220126 Milan, ItalyMicroarray platforms require analytical pipelines with modules for data pre-processing including data normalization, statistical analysis for identification of differentially expressed genes, cluster analysis, and functional annotation. We previously developed the Automated Microarray Data Analysis (AMDA, version 2.3.5) pipeline to process Affymetrix 3′ IVT GeneChips. The availability of newer technologies that demand open-source tools for microarray data analysis has impelled us to develop an updated multi-platform version, AMDA 2.13. It includes additional quality control metrics, annotation-driven (annotation grade of Affymetrix NetAffx) and signal-driven (Inter-Quartile Range) gene filtering, and approaches to experimental design. To enhance understanding of biological data, differentially expressed genes have been mapped into KEGG pathways. Finally, a more stable and user-friendly interface was designed to integrate the requirements for different platforms. AMDA 2.13 allows the analysis of Affymetrix (cartridges and plates) and whole transcript probe design (Gene 1.0/1.1 ST and Exon 1.0 ST GeneChips), Illumina Bead Arrays, and one-channel Agilent 4×44 arrays. Relative to early versions, it supports various experimental designs and delivers more insightful biological understanding and up-to-date annotations.https://www.future-science.com/doi/10.2144/0000113889data analysismicroarraysanalytical pipelinesautomationopen-source software
spellingShingle Dimos Kapetis
Ferdinando Clarelli
Federico Vitulli
Nicole Kerlero de Rosbo
Ottavio Beretta
Maria Foti
Paola Ricciardi-Castagnoli
Francesca Zolezzi
AMDA 2.13: A major update for automated cross-platform microarray data analysis
BioTechniques
data analysis
microarrays
analytical pipelines
automation
open-source software
title AMDA 2.13: A major update for automated cross-platform microarray data analysis
title_full AMDA 2.13: A major update for automated cross-platform microarray data analysis
title_fullStr AMDA 2.13: A major update for automated cross-platform microarray data analysis
title_full_unstemmed AMDA 2.13: A major update for automated cross-platform microarray data analysis
title_short AMDA 2.13: A major update for automated cross-platform microarray data analysis
title_sort amda 2 13 a major update for automated cross platform microarray data analysis
topic data analysis
microarrays
analytical pipelines
automation
open-source software
url https://www.future-science.com/doi/10.2144/0000113889
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