Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022
ABSTRACT The objective of this research was to investigate the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in a pediatric population in Shanghai, China. Whole-genome sequencing was conducted for 492 randomly selected MRSA isolates obtained from a pediatric hospital bet...
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American Society for Microbiology
2025-05-01
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| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00371-25 |
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| author | Xiaozhou Pan Jiao Zhang Fen Pan Chun Wang Huihong Qin Fangyuan Yu Tiandong Zhang Wenxin Liu Qianyue Wu Zhan Ma Wenhao Weng Liang Chen Fangyou Yu Hong Zhang Bingjie Wang |
| author_facet | Xiaozhou Pan Jiao Zhang Fen Pan Chun Wang Huihong Qin Fangyuan Yu Tiandong Zhang Wenxin Liu Qianyue Wu Zhan Ma Wenhao Weng Liang Chen Fangyou Yu Hong Zhang Bingjie Wang |
| author_sort | Xiaozhou Pan |
| collection | DOAJ |
| description | ABSTRACT The objective of this research was to investigate the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in a pediatric population in Shanghai, China. Whole-genome sequencing was conducted for 492 randomly selected MRSA isolates obtained from a pediatric hospital between 2013 and 2022. ST59 (37.4%), ST398 (22.4%), ST88 (5.7%), and ST22 (5.5%) were the predominant lineages among these children. While ST59 maintained a dominant annual proportion before 2017, the proportion of ST398 gradually increased from 2013 to 2016, with ST398 ultimately emerging as a prevalent clone with a proportion comparable to that of ST59 after 2017. Among the prevalent STs, the spa-SCCmec structure also experienced dynamic changes. Within ST59, the t437-IV subtype experienced a decline and has even been replaced by t172-IV in recent years. In ST398, the t011-V subtype appeared in 2014 and rapidly became the leading subtype. The antibiotic resistance profiles and virulence factors exhibited clone-related features. Compared with other prevalent lineages, ST59 presented high resistance to erythromycin and clindamycin, whereas ST398 presented relatively low resistance to common antimicrobial agents and fewer virulence determinants. Panton-Valentine leucocidin was more common in ST338 and ST1232, whereas toxic shock syndrome toxin was closely associated with ST1 and ST5. The MRSA cases could also be classified into community- and hospital-associated cases, with highly significant differences between the two in terms of demographic characteristics, clindamycin susceptibility, and virulence genes. In conclusion, this study revealed high genetic diversity and dynamic changes in the molecular epidemiology of pediatric MRSA isolates from Shanghai collected over a decade.IMPORTANCEMethicillin-resistant Staphylococcus aureus (MRSA) has emerged as a significant global health concern. Previous research on MRSA epidemiology has predominantly focused on adult populations or targeted specific infection sites, while there was limited research on the long-term evolution of MRSA from the pediatric population. This study addresses this knowledge gap by conducting a comprehensive, 10-year surveillance of pediatric MRSA isolates using whole-genome sequencing. We characterized the molecular typing, as well as the phenotypic and genotypic antimicrobial resistance profiles, and virulence factors present in MRSA isolates obtained from children. Our results highlight the imperative for continuous, vigilant monitoring of MRSA within the pediatric demographic to track its evolving genetic landscape. |
| format | Article |
| id | doaj-art-e35e6353f764403b9c9fe2cece69e47e |
| institution | OA Journals |
| issn | 2379-5077 |
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| publishDate | 2025-05-01 |
| publisher | American Society for Microbiology |
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| series | mSystems |
| spelling | doaj-art-e35e6353f764403b9c9fe2cece69e47e2025-08-20T02:32:53ZengAmerican Society for MicrobiologymSystems2379-50772025-05-0110510.1128/msystems.00371-25Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022Xiaozhou Pan0Jiao Zhang1Fen Pan2Chun Wang3Huihong Qin4Fangyuan Yu5Tiandong Zhang6Wenxin Liu7Qianyue Wu8Zhan Ma9Wenhao Weng10Liang Chen11Fangyou Yu12Hong Zhang13Bingjie Wang14Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, USADepartment of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, ChinaABSTRACT The objective of this research was to investigate the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in a pediatric population in Shanghai, China. Whole-genome sequencing was conducted for 492 randomly selected MRSA isolates obtained from a pediatric hospital between 2013 and 2022. ST59 (37.4%), ST398 (22.4%), ST88 (5.7%), and ST22 (5.5%) were the predominant lineages among these children. While ST59 maintained a dominant annual proportion before 2017, the proportion of ST398 gradually increased from 2013 to 2016, with ST398 ultimately emerging as a prevalent clone with a proportion comparable to that of ST59 after 2017. Among the prevalent STs, the spa-SCCmec structure also experienced dynamic changes. Within ST59, the t437-IV subtype experienced a decline and has even been replaced by t172-IV in recent years. In ST398, the t011-V subtype appeared in 2014 and rapidly became the leading subtype. The antibiotic resistance profiles and virulence factors exhibited clone-related features. Compared with other prevalent lineages, ST59 presented high resistance to erythromycin and clindamycin, whereas ST398 presented relatively low resistance to common antimicrobial agents and fewer virulence determinants. Panton-Valentine leucocidin was more common in ST338 and ST1232, whereas toxic shock syndrome toxin was closely associated with ST1 and ST5. The MRSA cases could also be classified into community- and hospital-associated cases, with highly significant differences between the two in terms of demographic characteristics, clindamycin susceptibility, and virulence genes. In conclusion, this study revealed high genetic diversity and dynamic changes in the molecular epidemiology of pediatric MRSA isolates from Shanghai collected over a decade.IMPORTANCEMethicillin-resistant Staphylococcus aureus (MRSA) has emerged as a significant global health concern. Previous research on MRSA epidemiology has predominantly focused on adult populations or targeted specific infection sites, while there was limited research on the long-term evolution of MRSA from the pediatric population. This study addresses this knowledge gap by conducting a comprehensive, 10-year surveillance of pediatric MRSA isolates using whole-genome sequencing. We characterized the molecular typing, as well as the phenotypic and genotypic antimicrobial resistance profiles, and virulence factors present in MRSA isolates obtained from children. Our results highlight the imperative for continuous, vigilant monitoring of MRSA within the pediatric demographic to track its evolving genetic landscape.https://journals.asm.org/doi/10.1128/msystems.00371-25methicillin-resistant Staphylococcus aureusgenome sequencingmolecular epidemiologychildrenantibiotics |
| spellingShingle | Xiaozhou Pan Jiao Zhang Fen Pan Chun Wang Huihong Qin Fangyuan Yu Tiandong Zhang Wenxin Liu Qianyue Wu Zhan Ma Wenhao Weng Liang Chen Fangyou Yu Hong Zhang Bingjie Wang Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 mSystems methicillin-resistant Staphylococcus aureus genome sequencing molecular epidemiology children antibiotics |
| title | Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 |
| title_full | Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 |
| title_fullStr | Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 |
| title_full_unstemmed | Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 |
| title_short | Longitudinal surveillance of the molecular evolution of methicillin-resistant Staphylococcus aureus isolates from pediatric patients in Shanghai, China, from 2013 to 2022 |
| title_sort | longitudinal surveillance of the molecular evolution of methicillin resistant staphylococcus aureus isolates from pediatric patients in shanghai china from 2013 to 2022 |
| topic | methicillin-resistant Staphylococcus aureus genome sequencing molecular epidemiology children antibiotics |
| url | https://journals.asm.org/doi/10.1128/msystems.00371-25 |
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