Differential Expression of Sphingosine-1-Phosphate Receptors in Abdominal Aortic Aneurysms
Objective. Inflammation plays a key role in the pathophysiology of abdominal aortic aneurysms (AAAs). Newly discovered Sphingosine-1-Phosphate Receptors (S1P receptors) are critical in modulating inflammatory response via prostaglandin production. The aim of the current study was to investigate the...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/643609 |
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| Summary: | Objective. Inflammation plays a key role in the pathophysiology of abdominal aortic aneurysms (AAAs). Newly discovered Sphingosine-1-Phosphate Receptors (S1P receptors) are critical in modulating inflammatory response via prostaglandin production. The aim of the current study was to investigate the expression of different S1P receptors in AAAs and compared with normal aortas at the protein level. Materials and Methods. Aortic specimens were harvested during aortic reconstructive surgery for the AAA group or during organ transplant for the control group. The protein expression of
S1P1, 2 and 3 in AAAs and normal aortas was assessed by Western blotting and immunohistochemical analysis. Results. There were 40 AAAs and 20 control aortas collected for the receptor analysis. For
Western blot analysis, S1P1 expression was not detected in either group; S1P2 protein was
constitutively detected in both types of aortas but its expression level was significantly decreased
by 73% (𝑃<0.05) in AAAs compared with the control group. In contrast, strong S1P3 expression
was detected in AAAs aortas but not in normal aortas. Immumohistochemical staining showed
similar results, except a weak S1P3 signal was detectable in normal aortas.
Conclusions. Western blot and staining results consistently showed the down-regulation of the
S1P2 protein with simultaneous up-regulation of the S1P3 protein in AAAs. Since those newly
discovered receptors play an important role in the inflammatory cascade, the modulating of S1P
signaling, particularly via S1P2 and S1P3, could represent novel therapeutic targets in future
AAA treatments. |
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| ISSN: | 0962-9351 1466-1861 |