Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases
The most emerging resistance mechanism against β-lactam antibiotics present in bacteria is the production of β-lactamases. The aim of this study was to explore the phytocompounds of Camellia sinensis (green tea) that can inhibit the activity of β-lactamases. Moreover, the antibacterial effect of its...
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Society of Eminent Biological Scientists
2025-01-01
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| Series: | International Journal of Applied and Experimental Biology |
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| Online Access: | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/111 |
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| author | Faiz Un Nisa Amina Javid Mehboob Ahmed |
| author_facet | Faiz Un Nisa Amina Javid Mehboob Ahmed |
| author_sort | Faiz Un Nisa |
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| description | The most emerging resistance mechanism against β-lactam antibiotics present in bacteria is the production of β-lactamases. The aim of this study was to explore the phytocompounds of Camellia sinensis (green tea) that can inhibit the activity of β-lactamases. Moreover, the antibacterial effect of its extract with a combination of antibiotics against resistant bacterial strains was also appraised. In silico docking was carried out against the resistance causing enzymes such as AmpC and SHV-1. Antimicrobial susceptibility testing against bacterial strains, i.e., Klebsiella pneumoniae and Escherichia coli, was performed. Then, for exploring the synergistic effects, both antibiotics and green tea extract were applied in combination. The docking studies revealed that the inhibitors like epigallocatechin gallate with AmpC, and myricetin with SHV-1 enzyme displayed high binding affinities of -8.2 kJ/mol, and -7.5 kJ/mol, respectively. The in vitro combination of C. sinensis extract with ampicillin and penicillin also potentiated the antibacterial activity of these antibiotics. Thus, the study elucidated that the phytochemicals of C. sinensis could inhibit β-lactamases produced by the pathogens. Also, it has enhanced antimicrobial effects when combined with antibiotics. |
| format | Article |
| id | doaj-art-e3513e32aace4fb286dd3db92fbde0fa |
| institution | OA Journals |
| issn | 2790-6523 2790-6531 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Society of Eminent Biological Scientists |
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| series | International Journal of Applied and Experimental Biology |
| spelling | doaj-art-e3513e32aace4fb286dd3db92fbde0fa2025-08-20T02:28:46ZengSociety of Eminent Biological ScientistsInternational Journal of Applied and Experimental Biology2790-65232790-65312025-01-0141414810.56612/ijaaeb.v1i1.111112Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamasesFaiz Un Nisa0Amina Javid1Mehboob Ahmed2Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahorea:1:{s:5:"en_US";s:82:"Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore";}Institute of Microbiology and Molecular Genetics, University of the Punjab, LahoreThe most emerging resistance mechanism against β-lactam antibiotics present in bacteria is the production of β-lactamases. The aim of this study was to explore the phytocompounds of Camellia sinensis (green tea) that can inhibit the activity of β-lactamases. Moreover, the antibacterial effect of its extract with a combination of antibiotics against resistant bacterial strains was also appraised. In silico docking was carried out against the resistance causing enzymes such as AmpC and SHV-1. Antimicrobial susceptibility testing against bacterial strains, i.e., Klebsiella pneumoniae and Escherichia coli, was performed. Then, for exploring the synergistic effects, both antibiotics and green tea extract were applied in combination. The docking studies revealed that the inhibitors like epigallocatechin gallate with AmpC, and myricetin with SHV-1 enzyme displayed high binding affinities of -8.2 kJ/mol, and -7.5 kJ/mol, respectively. The in vitro combination of C. sinensis extract with ampicillin and penicillin also potentiated the antibacterial activity of these antibiotics. Thus, the study elucidated that the phytochemicals of C. sinensis could inhibit β-lactamases produced by the pathogens. Also, it has enhanced antimicrobial effects when combined with antibiotics.https://submissions.eminentscientists.com/index.php/IJAEB/article/view/111bioinformaticsenzyme inhibitiongram-negativepathogensphytochemistryplant-derived compounds |
| spellingShingle | Faiz Un Nisa Amina Javid Mehboob Ahmed Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases International Journal of Applied and Experimental Biology bioinformatics enzyme inhibition gram-negative pathogens phytochemistry plant-derived compounds |
| title | Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases |
| title_full | Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases |
| title_fullStr | Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases |
| title_full_unstemmed | Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases |
| title_short | Green tea (Camellia sinensis) leaf extract inhibits the activity of β-lactamases |
| title_sort | green tea camellia sinensis leaf extract inhibits the activity of β lactamases |
| topic | bioinformatics enzyme inhibition gram-negative pathogens phytochemistry plant-derived compounds |
| url | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/111 |
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