Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance
Abstract Alcohol dehydrogenase has been studied in regulation of fungal growth and development, stress response and pathogenesis, but its function in T. asahii remains unexplored. In this study, we analyzed the ADH gene family in T. asahii for the first time, identifying six ADH genes and containing...
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2025-04-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11546-5 |
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| author | Zhen Liu Xiaoping Ma Xiangwen Zeng Zhiguo Li Ruiguo Liu Rongyan Luo Weichen Wang Muhammad Salman Tahir Chengdong Wang Yu Gu |
| author_facet | Zhen Liu Xiaoping Ma Xiangwen Zeng Zhiguo Li Ruiguo Liu Rongyan Luo Weichen Wang Muhammad Salman Tahir Chengdong Wang Yu Gu |
| author_sort | Zhen Liu |
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| description | Abstract Alcohol dehydrogenase has been studied in regulation of fungal growth and development, stress response and pathogenesis, but its function in T. asahii remains unexplored. In this study, we analyzed the ADH gene family in T. asahii for the first time, identifying six ADH genes and containing conserved ADH_N and ADH_Zinc_N domains. We constructed an overexpression strain of the most significantly differentially expressed gene TaADH_like and compared its phenotypes with those of the wild-type strain, focusing on colony morphology, biofilm biomass, stress response, drug resistance, and pathogenicity. The results showed that TaADH_like overexpression reduced sensitivity to hypoxic conditions, altered the hyphae-to-yeast transition, and led to slower growth, decreased colonization ability, reduced tissue damage, and lower lethality. Increased osmotic stress sensitivity and the involvement of the HOG MAPK pathway in the hyphae-to-yeast conversion contributed to the reduced colonization capacity of T. asahii. Furthermore, the overexpression of TaADH_like promoted biofilm formation and led to a slight enhancement in fluconazole resistance in T. asahii. This study is the first to elucidate the function of the alcohol dehydrogenase gene in T. asahii, providing a foundation for future genetic research on this pathogen. |
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| language | English |
| publishDate | 2025-04-01 |
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| series | BMC Genomics |
| spelling | doaj-art-e34dc4a8512247e0b19e3cf2c75fc1202025-08-20T02:11:51ZengBMCBMC Genomics1471-21642025-04-0126111610.1186/s12864-025-11546-5Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistanceZhen Liu0Xiaoping Ma1Xiangwen Zeng2Zhiguo Li3Ruiguo Liu4Rongyan Luo5Weichen Wang6Muhammad Salman Tahir7Chengdong Wang8Yu Gu9Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural UniversityChina Conservation and Research Center for the Giant PandaCollege of Life Sciences, Sichuan Agricultural UniversityAbstract Alcohol dehydrogenase has been studied in regulation of fungal growth and development, stress response and pathogenesis, but its function in T. asahii remains unexplored. In this study, we analyzed the ADH gene family in T. asahii for the first time, identifying six ADH genes and containing conserved ADH_N and ADH_Zinc_N domains. We constructed an overexpression strain of the most significantly differentially expressed gene TaADH_like and compared its phenotypes with those of the wild-type strain, focusing on colony morphology, biofilm biomass, stress response, drug resistance, and pathogenicity. The results showed that TaADH_like overexpression reduced sensitivity to hypoxic conditions, altered the hyphae-to-yeast transition, and led to slower growth, decreased colonization ability, reduced tissue damage, and lower lethality. Increased osmotic stress sensitivity and the involvement of the HOG MAPK pathway in the hyphae-to-yeast conversion contributed to the reduced colonization capacity of T. asahii. Furthermore, the overexpression of TaADH_like promoted biofilm formation and led to a slight enhancement in fluconazole resistance in T. asahii. This study is the first to elucidate the function of the alcohol dehydrogenase gene in T. asahii, providing a foundation for future genetic research on this pathogen.https://doi.org/10.1186/s12864-025-11546-5Trichosporon asahiiAlcohol dehydrogenaseADH gene familyHyphae-to-yeast conversionGene function |
| spellingShingle | Zhen Liu Xiaoping Ma Xiangwen Zeng Zhiguo Li Ruiguo Liu Rongyan Luo Weichen Wang Muhammad Salman Tahir Chengdong Wang Yu Gu Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance BMC Genomics Trichosporon asahii Alcohol dehydrogenase ADH gene family Hyphae-to-yeast conversion Gene function |
| title | Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance |
| title_full | Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance |
| title_fullStr | Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance |
| title_full_unstemmed | Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance |
| title_short | Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance |
| title_sort | identification of the adh gene family in trichosporon asahii and the role of taadh like in pathogenicity and fluconazole resistance |
| topic | Trichosporon asahii Alcohol dehydrogenase ADH gene family Hyphae-to-yeast conversion Gene function |
| url | https://doi.org/10.1186/s12864-025-11546-5 |
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