Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix
The high risks of traumatic cataract treatments promoted the development of the concept of autologous lens regeneration. Biochemical cues can influence the cellular behavior of stem cells, and in this case, biophysical cues may be the important factors in producing rapid activation of cellular behav...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1526943/full |
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| _version_ | 1841558659005415424 |
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| author | Yufan Liu Zuowei Wang Tianju Ma Yi Gao Wenqian Chen Zi Ye Zhaohui Li |
| author_facet | Yufan Liu Zuowei Wang Tianju Ma Yi Gao Wenqian Chen Zi Ye Zhaohui Li |
| author_sort | Yufan Liu |
| collection | DOAJ |
| description | The high risks of traumatic cataract treatments promoted the development of the concept of autologous lens regeneration. Biochemical cues can influence the cellular behavior of stem cells, and in this case, biophysical cues may be the important factors in producing rapid activation of cellular behavior. Here we bio-printed mesenchymal stem cells (MSCs) using a commonly used bioink sodium alginate-gelatin blends, and investigated the induction effect of MSC differentiation towards lens epithelial stem cells (LESCs) under a combination of biochemical cues and biophysical cues. It was found that biochemical cues in the porous three-dimensional (3D) matrix constructed using bioink sodium alginate-gelatin blends for bio-printing did not reduce the cell viability of loaded MSCs in the matrix by scanning electron microscope (SEM) observation and cell viability detection. Loaded MSCs in the matrix were consistently upregulated in the expression of proteins and genes involved in phenotypes and development signaling pathways of LESCs, as detected by polymerase chain reaction (PCR) with the support of biochemical cues. These results indicated that biophysical cues could rapidly activate the cellular behavior of MSCs differentiation, and biochemical cues could continuously induce MSCs differentiation towards LESCs. |
| format | Article |
| id | doaj-art-e34d145d3715463c9be559545f36b137 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-e34d145d3715463c9be559545f36b1372025-01-06T06:59:46ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.15269431526943Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrixYufan LiuZuowei WangTianju MaYi GaoWenqian ChenZi YeZhaohui LiThe high risks of traumatic cataract treatments promoted the development of the concept of autologous lens regeneration. Biochemical cues can influence the cellular behavior of stem cells, and in this case, biophysical cues may be the important factors in producing rapid activation of cellular behavior. Here we bio-printed mesenchymal stem cells (MSCs) using a commonly used bioink sodium alginate-gelatin blends, and investigated the induction effect of MSC differentiation towards lens epithelial stem cells (LESCs) under a combination of biochemical cues and biophysical cues. It was found that biochemical cues in the porous three-dimensional (3D) matrix constructed using bioink sodium alginate-gelatin blends for bio-printing did not reduce the cell viability of loaded MSCs in the matrix by scanning electron microscope (SEM) observation and cell viability detection. Loaded MSCs in the matrix were consistently upregulated in the expression of proteins and genes involved in phenotypes and development signaling pathways of LESCs, as detected by polymerase chain reaction (PCR) with the support of biochemical cues. These results indicated that biophysical cues could rapidly activate the cellular behavior of MSCs differentiation, and biochemical cues could continuously induce MSCs differentiation towards LESCs.https://www.frontiersin.org/articles/10.3389/fcell.2024.1526943/fullMSCs3D bio-printed matrixdifferentiationLESCscrystalline lens |
| spellingShingle | Yufan Liu Zuowei Wang Tianju Ma Yi Gao Wenqian Chen Zi Ye Zhaohui Li Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix Frontiers in Cell and Developmental Biology MSCs 3D bio-printed matrix differentiation LESCs crystalline lens |
| title | Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix |
| title_full | Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix |
| title_fullStr | Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix |
| title_full_unstemmed | Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix |
| title_short | Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix |
| title_sort | differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three dimensional bio printed matrix |
| topic | MSCs 3D bio-printed matrix differentiation LESCs crystalline lens |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1526943/full |
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