Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening
Abstract Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scena...
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| Format: | Article |
| Language: | English |
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Springer
2025-06-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06845-4 |
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| author | Lays Fernanda Nunes Dourado Fernanda Aparecida Silva Vieira Thomas Toshio Inoue Silvia Ligorio Fialho Lutiana Amaral de Melo Vinicius Viana Pereira Juliana Rios de Simoni Matheus Soares Siman Paulo Ferrara de Almeida Cunha Armando da Silva Cunha Júnior |
| author_facet | Lays Fernanda Nunes Dourado Fernanda Aparecida Silva Vieira Thomas Toshio Inoue Silvia Ligorio Fialho Lutiana Amaral de Melo Vinicius Viana Pereira Juliana Rios de Simoni Matheus Soares Siman Paulo Ferrara de Almeida Cunha Armando da Silva Cunha Júnior |
| author_sort | Lays Fernanda Nunes Dourado |
| collection | DOAJ |
| description | Abstract Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scenario, new approaches have been tested to obtain substitute tissues. Porcine cornea has been receiving increasing attention due to its ease of obtaining, biomechanical properties similar to those of human tissue and lower antigenicity. Based on this, the objective of this study was to evaluate the biocompatibility of porcine stroma decellularized by sodium dodecyl sulfate (SDS) through interlamellar implantation in rabbit corneas. The obtained results showed that the lenticule intrastromal implantation was successfully performed and did not elicit rejection. Furthermore, the implanted stroma was able to promote an increase in the thickness of the host cornea. Microscopic analyses revealed that the tissue was well-adhered and the collagen fibrils were more aligned on its periphery. Therefore, it is concluded that the implantation of decellularized porcine stroma occurred satisfactorily and represents a promising alternative to replace human tissue. Graphical Abstract |
| format | Article |
| id | doaj-art-e34cba3fddc644b39b26bf99b0da4078 |
| institution | DOAJ |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-e34cba3fddc644b39b26bf99b0da40782025-08-20T02:40:14ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-06-0136111210.1007/s10856-024-06845-4Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickeningLays Fernanda Nunes Dourado0Fernanda Aparecida Silva Vieira1Thomas Toshio Inoue2Silvia Ligorio Fialho3Lutiana Amaral de Melo4Vinicius Viana Pereira5Juliana Rios de Simoni6Matheus Soares Siman7Paulo Ferrara de Almeida Cunha8Armando da Silva Cunha Júnior9Faculdade de Farmácia, Universidade Federal de Minas GeraisFaculdade de Farmácia, Universidade Federal de Minas GeraisFaculdade de Farmácia, Universidade Federal de Minas GeraisDiretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel DiasDiretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel DiasFaculdade de Farmácia, Universidade Federal de Minas GeraisCentro de Saúde Ocular, Ed. Life CenterCentro de Saúde Ocular, Ed. Life CenterCentro de Saúde Ocular, Ed. Life CenterFaculdade de Farmácia, Universidade Federal de Minas GeraisAbstract Changes in collagen orientation and distribution on the corneas lead to the development of diseases characterized by progressive thinning, such as keratoconus. Part of people diagnosed with keratoconus require a corneal graft, which has availability as a major limiting factor. In this scenario, new approaches have been tested to obtain substitute tissues. Porcine cornea has been receiving increasing attention due to its ease of obtaining, biomechanical properties similar to those of human tissue and lower antigenicity. Based on this, the objective of this study was to evaluate the biocompatibility of porcine stroma decellularized by sodium dodecyl sulfate (SDS) through interlamellar implantation in rabbit corneas. The obtained results showed that the lenticule intrastromal implantation was successfully performed and did not elicit rejection. Furthermore, the implanted stroma was able to promote an increase in the thickness of the host cornea. Microscopic analyses revealed that the tissue was well-adhered and the collagen fibrils were more aligned on its periphery. Therefore, it is concluded that the implantation of decellularized porcine stroma occurred satisfactorily and represents a promising alternative to replace human tissue. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06845-4 |
| spellingShingle | Lays Fernanda Nunes Dourado Fernanda Aparecida Silva Vieira Thomas Toshio Inoue Silvia Ligorio Fialho Lutiana Amaral de Melo Vinicius Viana Pereira Juliana Rios de Simoni Matheus Soares Siman Paulo Ferrara de Almeida Cunha Armando da Silva Cunha Júnior Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening Journal of Materials Science: Materials in Medicine |
| title | Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| title_full | Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| title_fullStr | Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| title_full_unstemmed | Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| title_short | Interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| title_sort | interlamellar keratoplasty for implantation of decellularized porcine corneal lenticule in a rabbit for corneal thickening |
| url | https://doi.org/10.1007/s10856-024-06845-4 |
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