In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i>
Infections caused by multidrug-resistant organisms (MDRO) are linked to poor outcomes, particularly in patients with cirrhosis. The underlying mechanisms are not fully understood and may involve a different immune response against MDRO. This study aimed to compare the in vitro immune response betwee...
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MDPI AG
2025-05-01
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| author | Berta Cuyàs Elisabet Cantó Elisabet Sanchez-Ardid Elisenda Miró Edilmar Alvarado-Tapias Eva Román Maria Poca Ferran Navarro Andreu Ferrero-Gregori Maria Àngels Escorsell Silvia Vidal German Soriano |
| author_facet | Berta Cuyàs Elisabet Cantó Elisabet Sanchez-Ardid Elisenda Miró Edilmar Alvarado-Tapias Eva Román Maria Poca Ferran Navarro Andreu Ferrero-Gregori Maria Àngels Escorsell Silvia Vidal German Soriano |
| author_sort | Berta Cuyàs |
| collection | DOAJ |
| description | Infections caused by multidrug-resistant organisms (MDRO) are linked to poor outcomes, particularly in patients with cirrhosis. The underlying mechanisms are not fully understood and may involve a different immune response against MDRO. This study aimed to compare the in vitro immune response between multidrug-resistant (MDR) <i>Escherichia coli</i> and antibiotic-susceptible <i>E. coli</i> strains. Surface protein extract and DNA extract were obtained from MDR <i>E. coli</i> (n = 6) and antibiotic-susceptible <i>E. coli</i> (n = 6) strains isolated from infected patients with cirrhosis. The extracts were used to stimulate in vitro peripheral blood mononuclear cells from healthy donors. After 48 h, cytokine levels (IFN-γ, IL-1β, IL-10, IL-12p70, MCP-1, IL-8, IL-6, MIP-1α, and MIP-1β) were measured. We observed no significant differences in cytokine production between MDR and susceptible strains. However, we identified notable interindividual variability in cytokine production for most of the cytokines studied. Only IFN-γ and IL-6 in surface extract and MCP-1 in DNA extract showed similar levels across all donors. We conclude that the cytokine profiles induced by MDR <i>E. coli</i> in vitro were similar to those in susceptible strains. These findings suggest that the poor prognosis associated with MDR <i>E. coli</i> infections is not due to a differential immune response but rather to other factors. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Microorganisms |
| spelling | doaj-art-e345e954ccd64aa6a8658f9907c592952025-08-20T01:56:41ZengMDPI AGMicroorganisms2076-26072025-05-01135116410.3390/microorganisms13051164In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i>Berta Cuyàs0Elisabet Cantó1Elisabet Sanchez-Ardid2Elisenda Miró3Edilmar Alvarado-Tapias4Eva Román5Maria Poca6Ferran Navarro7Andreu Ferrero-Gregori8Maria Àngels Escorsell9Silvia Vidal10German Soriano11Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainInflammatory Diseases Department, Institut de Recerca Sant Pau (IR Sant Pau), 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainDepartment of Microbiology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainDepartment of Microbiology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainInflammatory Diseases Department, Institut de Recerca Sant Pau (IR Sant Pau), 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainInflammatory Diseases Department, Institut de Recerca Sant Pau (IR Sant Pau), 08041 Barcelona, SpainDepartment of Gastroenterology, Hospital de la Santa Creu i Sant Pau, C/Mas Casanovas 90, 08041 Barcelona, SpainInfections caused by multidrug-resistant organisms (MDRO) are linked to poor outcomes, particularly in patients with cirrhosis. The underlying mechanisms are not fully understood and may involve a different immune response against MDRO. This study aimed to compare the in vitro immune response between multidrug-resistant (MDR) <i>Escherichia coli</i> and antibiotic-susceptible <i>E. coli</i> strains. Surface protein extract and DNA extract were obtained from MDR <i>E. coli</i> (n = 6) and antibiotic-susceptible <i>E. coli</i> (n = 6) strains isolated from infected patients with cirrhosis. The extracts were used to stimulate in vitro peripheral blood mononuclear cells from healthy donors. After 48 h, cytokine levels (IFN-γ, IL-1β, IL-10, IL-12p70, MCP-1, IL-8, IL-6, MIP-1α, and MIP-1β) were measured. We observed no significant differences in cytokine production between MDR and susceptible strains. However, we identified notable interindividual variability in cytokine production for most of the cytokines studied. Only IFN-γ and IL-6 in surface extract and MCP-1 in DNA extract showed similar levels across all donors. We conclude that the cytokine profiles induced by MDR <i>E. coli</i> in vitro were similar to those in susceptible strains. These findings suggest that the poor prognosis associated with MDR <i>E. coli</i> infections is not due to a differential immune response but rather to other factors.https://www.mdpi.com/2076-2607/13/5/1164resistancecirrhosisimmune responseMDRO<i>Escherichia coli</i>cytokines |
| spellingShingle | Berta Cuyàs Elisabet Cantó Elisabet Sanchez-Ardid Elisenda Miró Edilmar Alvarado-Tapias Eva Román Maria Poca Ferran Navarro Andreu Ferrero-Gregori Maria Àngels Escorsell Silvia Vidal German Soriano In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> Microorganisms resistance cirrhosis immune response MDRO <i>Escherichia coli</i> cytokines |
| title | In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> |
| title_full | In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> |
| title_fullStr | In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> |
| title_full_unstemmed | In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> |
| title_short | In Vitro Immune Response of Mononuclear Cells to Multidrug-Resistant <i>Escherichia coli</i> |
| title_sort | in vitro immune response of mononuclear cells to multidrug resistant i escherichia coli i |
| topic | resistance cirrhosis immune response MDRO <i>Escherichia coli</i> cytokines |
| url | https://www.mdpi.com/2076-2607/13/5/1164 |
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