Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis

Abstract Non‐alcoholic fatty liver disease (NAFLD) is strongly associated with obesity and may progress to non‐alcoholic steatohepatitis (NASH) and liver fibrosis. The deficit of pharmacological therapies for the latter mainly results from an incomplete understanding of involved pathological mechani...

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Main Authors: Tenagne D Challa, Stephan Wueest, Fabrizio C Lucchini, Mara Dedual, Salvatore Modica, Marcela Borsigova, Christian Wolfrum, Matthias Blüher, Daniel Konrad
Format: Article
Language:English
Published: Springer Nature 2019-06-01
Series:EMBO Molecular Medicine
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Online Access:https://doi.org/10.15252/emmm.201810124
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author Tenagne D Challa
Stephan Wueest
Fabrizio C Lucchini
Mara Dedual
Salvatore Modica
Marcela Borsigova
Christian Wolfrum
Matthias Blüher
Daniel Konrad
author_facet Tenagne D Challa
Stephan Wueest
Fabrizio C Lucchini
Mara Dedual
Salvatore Modica
Marcela Borsigova
Christian Wolfrum
Matthias Blüher
Daniel Konrad
author_sort Tenagne D Challa
collection DOAJ
description Abstract Non‐alcoholic fatty liver disease (NAFLD) is strongly associated with obesity and may progress to non‐alcoholic steatohepatitis (NASH) and liver fibrosis. The deficit of pharmacological therapies for the latter mainly results from an incomplete understanding of involved pathological mechanisms. Herein, we identify apoptosis signal‐regulating kinase 1 (ASK1) as a suppressor of NASH and fibrosis formation. High‐fat diet‐fed and aged chow‐fed liver‐specific ASK1‐knockout mice develop a higher degree of hepatic steatosis, inflammation, and fibrosis compared to controls. In addition, pharmacological inhibition of ASK1 increased hepatic lipid accumulation in wild‐type mice. In line, liver‐specific ASK1 overexpression protected mice from the development of high‐fat diet‐induced hepatic steatosis and carbon tetrachloride‐induced fibrosis. Mechanistically, ASK1 depletion blunts autophagy, thereby enhancing lipid droplet accumulation and liver fibrosis. In human livers of lean and obese subjects, ASK1 expression correlated negatively with liver fat content and NASH scores, but positively with markers for autophagy. Taken together, ASK1 may be a novel therapeutic target to tackle NAFLD and liver fibrosis.
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issn 1757-4676
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spelling doaj-art-e34078afbfda453eaefc34f8adc5adcd2025-08-20T02:11:22ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842019-06-01111011710.15252/emmm.201810124Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosisTenagne D Challa0Stephan Wueest1Fabrizio C Lucchini2Mara Dedual3Salvatore Modica4Marcela Borsigova5Christian Wolfrum6Matthias Blüher7Daniel Konrad8Division of Pediatric Endocrinology and Diabetology, University Children's HospitalDivision of Pediatric Endocrinology and Diabetology, University Children's HospitalDivision of Pediatric Endocrinology and Diabetology, University Children's HospitalDivision of Pediatric Endocrinology and Diabetology, University Children's HospitalInstitute of Food, Nutrition and Health, ETH ZurichDivision of Pediatric Endocrinology and Diabetology, University Children's HospitalInstitute of Food, Nutrition and Health, ETH ZurichDepartment of Medicine, University of LeipzigDivision of Pediatric Endocrinology and Diabetology, University Children's HospitalAbstract Non‐alcoholic fatty liver disease (NAFLD) is strongly associated with obesity and may progress to non‐alcoholic steatohepatitis (NASH) and liver fibrosis. The deficit of pharmacological therapies for the latter mainly results from an incomplete understanding of involved pathological mechanisms. Herein, we identify apoptosis signal‐regulating kinase 1 (ASK1) as a suppressor of NASH and fibrosis formation. High‐fat diet‐fed and aged chow‐fed liver‐specific ASK1‐knockout mice develop a higher degree of hepatic steatosis, inflammation, and fibrosis compared to controls. In addition, pharmacological inhibition of ASK1 increased hepatic lipid accumulation in wild‐type mice. In line, liver‐specific ASK1 overexpression protected mice from the development of high‐fat diet‐induced hepatic steatosis and carbon tetrachloride‐induced fibrosis. Mechanistically, ASK1 depletion blunts autophagy, thereby enhancing lipid droplet accumulation and liver fibrosis. In human livers of lean and obese subjects, ASK1 expression correlated negatively with liver fat content and NASH scores, but positively with markers for autophagy. Taken together, ASK1 may be a novel therapeutic target to tackle NAFLD and liver fibrosis.https://doi.org/10.15252/emmm.201810124autophagyhigh‐fat dietNASH
spellingShingle Tenagne D Challa
Stephan Wueest
Fabrizio C Lucchini
Mara Dedual
Salvatore Modica
Marcela Borsigova
Christian Wolfrum
Matthias Blüher
Daniel Konrad
Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
EMBO Molecular Medicine
autophagy
high‐fat diet
NASH
title Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
title_full Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
title_fullStr Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
title_full_unstemmed Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
title_short Liver ASK1 protects from non‐alcoholic fatty liver disease and fibrosis
title_sort liver ask1 protects from non alcoholic fatty liver disease and fibrosis
topic autophagy
high‐fat diet
NASH
url https://doi.org/10.15252/emmm.201810124
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