Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis
Abstract In plants, the developing cell plate which is characterized by a series of anionic lipids, undergoes dramatic morphological change for successful cytokinesis. However, the mechanisms underlying these alterations, and the roles of anionic lipids such as phosphatidylinositol-4-phosphate (PI4P...
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| Format: | Article |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-62067-4 |
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| author | Yu Luo Yu-Fang Tian Hui-Ru Liu Wei-Cai Yang |
| author_facet | Yu Luo Yu-Fang Tian Hui-Ru Liu Wei-Cai Yang |
| author_sort | Yu Luo |
| collection | DOAJ |
| description | Abstract In plants, the developing cell plate which is characterized by a series of anionic lipids, undergoes dramatic morphological change for successful cytokinesis. However, the mechanisms underlying these alterations, and the roles of anionic lipids such as phosphatidylinositol-4-phosphate (PI4P), phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), and phosphatidylserine (PS) during cell division remain poorly understood. Here we present that changes in anionic lipid composition have a profound effect on cell plate development: deprivation of phosphatidylinositides (PIPs) leads to incomplete cytokinesis through distorted cell-plate architecture. Our data demonstrate that PI4P shapes cell plate membrane morphology through flippase-regulated PS flipping inhibition, while PI(4,5)P2 functions in the recruitment of dynamin-related protein 1A (DRP1A) and the constriction region formation; depletion of PIPs causes cell plate tubulation and flattening failure. We propose a model in which PI4P regulates the level and distribution of PS, while PI(4,5)P2 mediates the localization of DRP1A; together, they coordinate cell plate morphology to ensure successful cytokinesis in plant cells. |
| format | Article |
| id | doaj-art-e32ee5dc84414a16b2833294defdcdd1 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e32ee5dc84414a16b2833294defdcdd12025-08-20T03:43:14ZengNature PortfolioNature Communications2041-17232025-07-0116111610.1038/s41467-025-62067-4Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in ArabidopsisYu Luo0Yu-Fang Tian1Hui-Ru Liu2Wei-Cai Yang3State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract In plants, the developing cell plate which is characterized by a series of anionic lipids, undergoes dramatic morphological change for successful cytokinesis. However, the mechanisms underlying these alterations, and the roles of anionic lipids such as phosphatidylinositol-4-phosphate (PI4P), phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), and phosphatidylserine (PS) during cell division remain poorly understood. Here we present that changes in anionic lipid composition have a profound effect on cell plate development: deprivation of phosphatidylinositides (PIPs) leads to incomplete cytokinesis through distorted cell-plate architecture. Our data demonstrate that PI4P shapes cell plate membrane morphology through flippase-regulated PS flipping inhibition, while PI(4,5)P2 functions in the recruitment of dynamin-related protein 1A (DRP1A) and the constriction region formation; depletion of PIPs causes cell plate tubulation and flattening failure. We propose a model in which PI4P regulates the level and distribution of PS, while PI(4,5)P2 mediates the localization of DRP1A; together, they coordinate cell plate morphology to ensure successful cytokinesis in plant cells.https://doi.org/10.1038/s41467-025-62067-4 |
| spellingShingle | Yu Luo Yu-Fang Tian Hui-Ru Liu Wei-Cai Yang Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis Nature Communications |
| title | Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis |
| title_full | Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis |
| title_fullStr | Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis |
| title_full_unstemmed | Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis |
| title_short | Phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in Arabidopsis |
| title_sort | phosphatidylinositides regulate the cell plate morphology transition during cytokinesis in arabidopsis |
| url | https://doi.org/10.1038/s41467-025-62067-4 |
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