A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production
Developing a robust and safe carrier for enzyme immobilization is crucial for their application in the food processing industry. In this study, a novel crosslinked chitosan microspheres (CSMs) were prepared using glutaraldehyde (GA) as the crosslinking agent, using a newly developed emulsification-n...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524010186 |
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| author | Xiao-Yan Yin Rui-Fan Yang Zhong-Hua Yang |
| author_facet | Xiao-Yan Yin Rui-Fan Yang Zhong-Hua Yang |
| author_sort | Xiao-Yan Yin |
| collection | DOAJ |
| description | Developing a robust and safe carrier for enzyme immobilization is crucial for their application in the food processing industry. In this study, a novel crosslinked chitosan microspheres (CSMs) were prepared using glutaraldehyde (GA) as the crosslinking agent, using a newly developed emulsification-neutralization combined method. Nuclease P1 (NP1) was immobilized onto these microspheres, the maximum activity of NP1@CSMs-GA reach to 53,859.4 U/g. The activity recovery yield reach to 75 %. Compared to the free NP1, the stability of NP1@CSMs-GA was significantly enhanced. Its vmax and Km is 895.71 mg/(g·min) and 77.27 mg/mL respectively. This NP1@CSMs-GA was utilized in production of nucleotides through hydrolysis of RNA. In BSTR, NP1@CSMs-GA retained more than 75.1 % initial activity after 10 cycles of reuse. Moreover, in PBR, the RNA hydrolysis conversion rate maintained 81 % after 24 h of continuous operation. These results demonstrate that NP1@CSMs-GA exhibits excellent reusability and production stability in practical processes. |
| format | Article |
| id | doaj-art-e32929c79494422b9fc93647b86a97b4 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-e32929c79494422b9fc93647b86a97b42025-02-12T05:32:08ZengElsevierFood Chemistry: X2590-15752025-01-0125102130A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide productionXiao-Yan Yin0Rui-Fan Yang1Zhong-Hua Yang2Xingzhi College, Zhejiang Normal University, Jinhua 321100, ChinaSchool of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaXingzhi College, Zhejiang Normal University, Jinhua 321100, China; Corresponding author.Developing a robust and safe carrier for enzyme immobilization is crucial for their application in the food processing industry. In this study, a novel crosslinked chitosan microspheres (CSMs) were prepared using glutaraldehyde (GA) as the crosslinking agent, using a newly developed emulsification-neutralization combined method. Nuclease P1 (NP1) was immobilized onto these microspheres, the maximum activity of NP1@CSMs-GA reach to 53,859.4 U/g. The activity recovery yield reach to 75 %. Compared to the free NP1, the stability of NP1@CSMs-GA was significantly enhanced. Its vmax and Km is 895.71 mg/(g·min) and 77.27 mg/mL respectively. This NP1@CSMs-GA was utilized in production of nucleotides through hydrolysis of RNA. In BSTR, NP1@CSMs-GA retained more than 75.1 % initial activity after 10 cycles of reuse. Moreover, in PBR, the RNA hydrolysis conversion rate maintained 81 % after 24 h of continuous operation. These results demonstrate that NP1@CSMs-GA exhibits excellent reusability and production stability in practical processes.http://www.sciencedirect.com/science/article/pii/S2590157524010186Nucleic acid hydrolysisNuclease P1NucleotideEnzyme immobilizationFood processing enzyme |
| spellingShingle | Xiao-Yan Yin Rui-Fan Yang Zhong-Hua Yang A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production Food Chemistry: X Nucleic acid hydrolysis Nuclease P1 Nucleotide Enzyme immobilization Food processing enzyme |
| title | A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| title_full | A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| title_fullStr | A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| title_full_unstemmed | A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| title_short | A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| title_sort | novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production |
| topic | Nucleic acid hydrolysis Nuclease P1 Nucleotide Enzyme immobilization Food processing enzyme |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524010186 |
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