Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway
Abstract Qingdai (QD), derived from various plant sources, is commonly used in traditional Chinese medicine for ulcerative colitis (UC) treatment. However, the clinical efficacy and mechanisms of orally administered QD remain unclear. This study aims to evaluate QD’s efficacy in UC treatment and unc...
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BMC
2024-11-01
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| Series: | Journal of Inflammation |
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| Online Access: | https://doi.org/10.1186/s12950-024-00413-x |
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| author | Sizhen Gu Yan Xue Xiaowen Liu Yini Tang Dong Wang Dongmei Wu Mingrong Yao Zehua Xia Sen Yang Gan Cai Shigui Xue Danbo Dou |
| author_facet | Sizhen Gu Yan Xue Xiaowen Liu Yini Tang Dong Wang Dongmei Wu Mingrong Yao Zehua Xia Sen Yang Gan Cai Shigui Xue Danbo Dou |
| author_sort | Sizhen Gu |
| collection | DOAJ |
| description | Abstract Qingdai (QD), derived from various plant sources, is commonly used in traditional Chinese medicine for ulcerative colitis (UC) treatment. However, the clinical efficacy and mechanisms of orally administered QD remain unclear. This study aims to evaluate QD’s efficacy in UC treatment and uncover its active components and mechanisms. A randomized controlled trial compared QD to Adisa, followed by UPLC-Q-TOF/MS and network pharmacology analyses to identify QD’s core components and targets. In vivo experiments on a UC mouse model explored QD’s impact on the AHR-Th17/Treg pathway using PCR, WB, ELISA, and flow cytometry. Results showed QD’s efficacy in UC treatment, with mucosal healing and remission comparable to Adisa. UPLC-Q-TOF/MS identified 16 core components in mouse colon tissue, with network pharmacology revealing 67 targets, potentially involving the IL-17 signaling pathway and Th17 cell differentiation. QD and its components up-regulated AHR, CYP1A1, and Foxp3, while down-regulating RORγt. Additionally, QD modulated pro-inflammatory (IL-6, IL-17 A) and anti-inflammatory (IL-10, TGF-β1) factors, and Treg/Th17 cell ratios in LPMC. Oral QD administration effectively promoted mucosal healing and improved UC symptoms, potentially through AHR-Th17/Treg pathway regulation. |
| format | Article |
| id | doaj-art-e32309fc633d44298f9a141bbd854e63 |
| institution | DOAJ |
| issn | 1476-9255 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Inflammation |
| spelling | doaj-art-e32309fc633d44298f9a141bbd854e632025-08-20T02:49:56ZengBMCJournal of Inflammation1476-92552024-11-0121111410.1186/s12950-024-00413-xClinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathwaySizhen Gu0Yan Xue1Xiaowen Liu2Yini Tang3Dong Wang4Dongmei Wu5Mingrong Yao6Zehua Xia7Sen Yang8Gan Cai9Shigui Xue10Danbo Dou11Department of Traditional Chinese Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Traditional Chinese Medicine, Shanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Centre), ChinaDepartment of Traditional Chinese Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Digestive Endoscopy Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Gastroenterology, The second hospital of Hebei Medical UniversityDepartment of Pathology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Anorectal, Shuguang Hospital affiliated to Shanghai University of traditional Chinese MedicineDepartment of Clinical Lab, Shuguang Hospital affiliated to Shanghai University of traditional Chinese MedicineDepartment of Gastroenterology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Digestive Endoscopy Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineDepartment of Traditional Chinese Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineAbstract Qingdai (QD), derived from various plant sources, is commonly used in traditional Chinese medicine for ulcerative colitis (UC) treatment. However, the clinical efficacy and mechanisms of orally administered QD remain unclear. This study aims to evaluate QD’s efficacy in UC treatment and uncover its active components and mechanisms. A randomized controlled trial compared QD to Adisa, followed by UPLC-Q-TOF/MS and network pharmacology analyses to identify QD’s core components and targets. In vivo experiments on a UC mouse model explored QD’s impact on the AHR-Th17/Treg pathway using PCR, WB, ELISA, and flow cytometry. Results showed QD’s efficacy in UC treatment, with mucosal healing and remission comparable to Adisa. UPLC-Q-TOF/MS identified 16 core components in mouse colon tissue, with network pharmacology revealing 67 targets, potentially involving the IL-17 signaling pathway and Th17 cell differentiation. QD and its components up-regulated AHR, CYP1A1, and Foxp3, while down-regulating RORγt. Additionally, QD modulated pro-inflammatory (IL-6, IL-17 A) and anti-inflammatory (IL-10, TGF-β1) factors, and Treg/Th17 cell ratios in LPMC. Oral QD administration effectively promoted mucosal healing and improved UC symptoms, potentially through AHR-Th17/Treg pathway regulation.https://doi.org/10.1186/s12950-024-00413-xUlcerative ColitisQingdaiClinical efficacyUPLC-Q-TOF-MSAHRTh17/Treg |
| spellingShingle | Sizhen Gu Yan Xue Xiaowen Liu Yini Tang Dong Wang Dongmei Wu Mingrong Yao Zehua Xia Sen Yang Gan Cai Shigui Xue Danbo Dou Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway Journal of Inflammation Ulcerative Colitis Qingdai Clinical efficacy UPLC-Q-TOF-MS AHR Th17/Treg |
| title | Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway |
| title_full | Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway |
| title_fullStr | Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway |
| title_full_unstemmed | Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway |
| title_short | Clinical, pharmacology and in vivo studies of QingDai (indigo naturalis) promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the AHR-Th17/Treg pathway |
| title_sort | clinical pharmacology and in vivo studies of qingdai indigo naturalis promotes mucosal healing and symptom improvement in ulcerative colitis by regulating the ahr th17 treg pathway |
| topic | Ulcerative Colitis Qingdai Clinical efficacy UPLC-Q-TOF-MS AHR Th17/Treg |
| url | https://doi.org/10.1186/s12950-024-00413-x |
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