Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows
Glycogen synthase 2 (GYS2) encodes liver glycogen synthase, a rate limiting enzyme in glycogen metabolism. Our preliminary work suggested that GYS2 was a candidate gene affecting milk production traits by analyzing the liver proteome of dairy cows. Herein, this research identified single nucleotide...
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2432966 |
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| author | Wen Ye Ao Chen Lingna Xu Dongxiao Sun Bo Han |
| author_facet | Wen Ye Ao Chen Lingna Xu Dongxiao Sun Bo Han |
| author_sort | Wen Ye |
| collection | DOAJ |
| description | Glycogen synthase 2 (GYS2) encodes liver glycogen synthase, a rate limiting enzyme in glycogen metabolism. Our preliminary work suggested that GYS2 was a candidate gene affecting milk production traits by analyzing the liver proteome of dairy cows. Herein, this research identified single nucleotide polymorphisms (SNPs) of GYS2, analyzed their genetic effects on traits of dairy cattle, and speculated the pathogenic mechanism through functional prediction of key mutation sites. Seven SNPs were found by resequencing and the association analysis showed that these SNPs were significantly associated with 305-day milk yield, fat yield, protein yield or fat percentage (p-value ≤ 0.0488). Six SNPs among them formed two haplotype blocks and they were associated with 305-day milk yield, fat yield, protein yield or fat percentage (p-value ≤ 0.0349). Furthermore, 5:g.88602007G > A and 5:g.88602026G > A were predicted to change the transcription factor binding sites (TFBSs), which might regulate the expression of GYS2. The missense mutation site, 5:g.88602535G > T, changed the secondary structure of mRNA and the secondary and tertiary structure of protein. In summary, the GYS2 was proved to have genetic effect on milk production traits, and its valuable seven SNPs, could provide more useful genetic information for molecular breeding of dairy cows. |
| format | Article |
| id | doaj-art-e322a3944a80434b93e893298e175209 |
| institution | Kabale University |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Animal Biotechnology |
| spelling | doaj-art-e322a3944a80434b93e893298e1752092025-08-20T03:51:30ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2432966Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cowsWen Ye0Ao Chen1Lingna Xu2Dongxiao Sun3Bo Han4Department of Animal Genetics and Breeding, College of Animal Science and Technology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaGlycogen synthase 2 (GYS2) encodes liver glycogen synthase, a rate limiting enzyme in glycogen metabolism. Our preliminary work suggested that GYS2 was a candidate gene affecting milk production traits by analyzing the liver proteome of dairy cows. Herein, this research identified single nucleotide polymorphisms (SNPs) of GYS2, analyzed their genetic effects on traits of dairy cattle, and speculated the pathogenic mechanism through functional prediction of key mutation sites. Seven SNPs were found by resequencing and the association analysis showed that these SNPs were significantly associated with 305-day milk yield, fat yield, protein yield or fat percentage (p-value ≤ 0.0488). Six SNPs among them formed two haplotype blocks and they were associated with 305-day milk yield, fat yield, protein yield or fat percentage (p-value ≤ 0.0349). Furthermore, 5:g.88602007G > A and 5:g.88602026G > A were predicted to change the transcription factor binding sites (TFBSs), which might regulate the expression of GYS2. The missense mutation site, 5:g.88602535G > T, changed the secondary structure of mRNA and the secondary and tertiary structure of protein. In summary, the GYS2 was proved to have genetic effect on milk production traits, and its valuable seven SNPs, could provide more useful genetic information for molecular breeding of dairy cows.https://www.tandfonline.com/doi/10.1080/10495398.2024.2432966GYS2SNPgenetic effectmilk production traitsdairy cattle |
| spellingShingle | Wen Ye Ao Chen Lingna Xu Dongxiao Sun Bo Han Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows Animal Biotechnology GYS2 SNP genetic effect milk production traits dairy cattle |
| title | Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows |
| title_full | Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows |
| title_fullStr | Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows |
| title_full_unstemmed | Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows |
| title_short | Single nucleotide polymorphisms of GYS2 gene and its association with milk production traits of dairy cows |
| title_sort | single nucleotide polymorphisms of gys2 gene and its association with milk production traits of dairy cows |
| topic | GYS2 SNP genetic effect milk production traits dairy cattle |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2432966 |
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