Determination of the Infection Dynamics of <i>Escherichia coli</i> O157:H7 by Bacteriophage ΦV10

Determination of the Infection Dynamics of <i>Escherichia coli</i> O157:H7 by Bacteriophage ΦV10

ΦV10 is an <i>Escherichia coli</i> O157:H7-specific bacteriophage that has been used to develop luminescent reporter assays for the detection of this important foodborne pathogen. Previous work demonstrated the specificity of ΦV10 for infection of <i>E.coli</i> O157:H7 throug...

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Bibliographic Details
Main Authors: Michael F. Oats, Claudia P. Coronel-Aguilera, Bruce M. Applegate, Laszlo N. Csonka, Arun K. Bhunia, Andrew G. Gehring, George C. Paoli
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Foods
Subjects:
bacteriophage
<i>E. coli</i> O157:H7
ΦV10
phage binding
Online Access:https://www.mdpi.com/2304-8158/14/4/617
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Summary:ΦV10 is an <i>Escherichia coli</i> O157:H7-specific bacteriophage that has been used to develop luminescent reporter assays for the detection of this important foodborne pathogen. Previous work demonstrated the specificity of ΦV10 for infection of <i>E.coli</i> O157:H7 through interaction with the O157 antigen. In addition, modification of the lipopolysaccharide (LPS) via O-acetylation prevents ΦV10 infection in an <i>E. coli</i> O157:H7 expressing a phage-encoded O-acetylase gene. Through assays for phage binding, plaque formation, and lysogeny using non-O157:H7 and O157: non-H7 strains, as well as complementation of an O157:H<sup>−</sup> strain, it is demonstrated in this study that both the somatic O157 antigen and flagellar H7 antigen are required for productive infection of <i>E. coli</i> O157:H7 by ΦV10. Together, the results indicate that the O157 antigen is required for phage binding and that the H7 antigen is necessary to complete the infection process.
ISSN:2304-8158

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