Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis
Functions and mechanisms of cyclic nucleotide-gated ion channel (CNGC) in plant immunity against necrotrophic pathogens remain largely unknown. In this study, we explored the functions of AtCNGC3 in immunity against Sclerotinia sclerotiorum, a typical necrotrophic pathogen, through identifying subce...
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Zhejiang University Press
2022-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.11.291 |
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| author | LIU Mengjiao YI Hang CAI Xinzhong |
| author_facet | LIU Mengjiao YI Hang CAI Xinzhong |
| author_sort | LIU Mengjiao |
| collection | DOAJ |
| description | Functions and mechanisms of cyclic nucleotide-gated ion channel (CNGC) in plant immunity against necrotrophic pathogens remain largely unknown. In this study, we explored the functions of AtCNGC3 in immunity against Sclerotinia sclerotiorum, a typical necrotrophic pathogen, through identifying subcellular localization of its expression product, defining its expression patterns and genetically analyzing its function in immunity. The results showed that AtCNGC3 protein was localized in plasma membrane. AtCNGC3 was highly expressed in all analyzed tissues of Arabidopsis thaliana without showing any tissue specificity. Sclerotinia sclerotiorum inoculation strongly and constantly induced the expression of AtCNGC3 gene. The Atcngc3 mutant plants were more susceptible to S. sclerotiorum, while AtCNGC3 overexpression (AtCNGC3-OE) plants exhibited enhanced resistance to S. sclerotiorum, indicating that AtCNGC3 positively regulates the resistance to S. sclerotiorum. Seedlings soaking with Arabidopsis thaliana plant elicitor peptide 1 (AtPep1) could rapidly induce AtCNGC3 gene expression. AtPep1-elicited reactive oxygen species (ROS) burst was weakened in the Atcngc3 mutant seedlings while enhanced in the AtCNGC3-OE plants, demonstrating that AtCNGC3 positively regulates AtPep1-induced ROS burst. Collectively, our results reveal that AtCNGC3 positively regulates immunity against the necrotrophic fungal pathogen S. sclerotiorum in Arabidopsis, thereby providing some insights into the functions of CNGC in plant immunity. |
| format | Article |
| id | doaj-art-e30e65407b3442e68d53cdad98fb11cc |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-e30e65407b3442e68d53cdad98fb11cc2025-08-20T03:58:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-10-014859460410.3785/j.issn.1008-9209.2021.11.29110089209Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in ArabidopsisLIU MengjiaoYI HangCAI XinzhongFunctions and mechanisms of cyclic nucleotide-gated ion channel (CNGC) in plant immunity against necrotrophic pathogens remain largely unknown. In this study, we explored the functions of AtCNGC3 in immunity against Sclerotinia sclerotiorum, a typical necrotrophic pathogen, through identifying subcellular localization of its expression product, defining its expression patterns and genetically analyzing its function in immunity. The results showed that AtCNGC3 protein was localized in plasma membrane. AtCNGC3 was highly expressed in all analyzed tissues of Arabidopsis thaliana without showing any tissue specificity. Sclerotinia sclerotiorum inoculation strongly and constantly induced the expression of AtCNGC3 gene. The Atcngc3 mutant plants were more susceptible to S. sclerotiorum, while AtCNGC3 overexpression (AtCNGC3-OE) plants exhibited enhanced resistance to S. sclerotiorum, indicating that AtCNGC3 positively regulates the resistance to S. sclerotiorum. Seedlings soaking with Arabidopsis thaliana plant elicitor peptide 1 (AtPep1) could rapidly induce AtCNGC3 gene expression. AtPep1-elicited reactive oxygen species (ROS) burst was weakened in the Atcngc3 mutant seedlings while enhanced in the AtCNGC3-OE plants, demonstrating that AtCNGC3 positively regulates AtPep1-induced ROS burst. Collectively, our results reveal that AtCNGC3 positively regulates immunity against the necrotrophic fungal pathogen S. sclerotiorum in Arabidopsis, thereby providing some insights into the functions of CNGC in plant immunity.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.11.291cyclic nucleotide-gated ion channels<italic>Sclerotinia sclerotiorum</italic> disease resistanceplant immunityreactive oxygen species |
| spellingShingle | LIU Mengjiao YI Hang CAI Xinzhong Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis 浙江大学学报. 农业与生命科学版 cyclic nucleotide-gated ion channels <italic>Sclerotinia sclerotiorum</italic> disease resistance plant immunity reactive oxygen species |
| title | Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis |
| title_full | Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis |
| title_fullStr | Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis |
| title_full_unstemmed | Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis |
| title_short | Cyclic nucleotide-gated ion channel gene CNGC3 positively regulates immunity against Sclerotinia sclerotiorum in Arabidopsis |
| title_sort | cyclic nucleotide gated ion channel gene cngc3 positively regulates immunity against sclerotinia sclerotiorum in arabidopsis |
| topic | cyclic nucleotide-gated ion channels <italic>Sclerotinia sclerotiorum</italic> disease resistance plant immunity reactive oxygen species |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.11.291 |
| work_keys_str_mv | AT liumengjiao cyclicnucleotidegatedionchannelgenecngc3positivelyregulatesimmunityagainstsclerotiniasclerotioruminarabidopsis AT yihang cyclicnucleotidegatedionchannelgenecngc3positivelyregulatesimmunityagainstsclerotiniasclerotioruminarabidopsis AT caixinzhong cyclicnucleotidegatedionchannelgenecngc3positivelyregulatesimmunityagainstsclerotiniasclerotioruminarabidopsis |