Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection
Abstract Potyvirids are the largest group of plant RNA viruses. Pelota, a core component of RNA quality controls (RQC), promotes the degradation of potyvirids’ genomic RNA by recognizing a specific G1-2A6-7 motif. Here we demonstrate that the viral RNA-dependent RNA polymerase, NIb, acts as a SUMOyl...
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55288-6 |
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| author | Linhao Ge Mingxuan Jia Hongying Shan Weifang Gao Lu Jiang Hongguang Cui Xiaofei Cheng Marilyne Uzest Xueping Zhou Aiming Wang Fangfang Li |
| author_facet | Linhao Ge Mingxuan Jia Hongying Shan Weifang Gao Lu Jiang Hongguang Cui Xiaofei Cheng Marilyne Uzest Xueping Zhou Aiming Wang Fangfang Li |
| author_sort | Linhao Ge |
| collection | DOAJ |
| description | Abstract Potyvirids are the largest group of plant RNA viruses. Pelota, a core component of RNA quality controls (RQC), promotes the degradation of potyvirids’ genomic RNA by recognizing a specific G1-2A6-7 motif. Here we demonstrate that the viral RNA-dependent RNA polymerase, NIb, acts as a SUMOylation decoy to effectively reduce Pelota SUMOylation by competing with SCE1 to inhibit Pelota-mediated RQC. TuMV NIb is comprised of two functional SUMO interacting motif (SIM): SIM2 and SIM3. The former is identified as the key site for NIb’s SUMOylation by SUMO3, whereas the latter is responsible for the interaction with SCE1. These two SIMs are conserved among the majority of potyvirids-encoded NIbs. Thus, virus protein-mediated SUMOylation decoy strategy to suppress host defense may be a common feature in plant virus pathosystems. These findings highlight a dynamic interplay between plant defense mechanism and viral counter-strategy by orchestrating the post-translational modifications of virus and host defense components. |
| format | Article |
| id | doaj-art-e3083d883dbd440182d3dc76693bc057 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e3083d883dbd440182d3dc76693bc0572025-08-20T02:46:07ZengNature PortfolioNature Communications2041-17232025-01-0116111510.1038/s41467-024-55288-6Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infectionLinhao Ge0Mingxuan Jia1Hongying Shan2Weifang Gao3Lu Jiang4Hongguang Cui5Xiaofei Cheng6Marilyne Uzest7Xueping Zhou8Aiming Wang9Fangfang Li10State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education and College of Plant Protection, Hainan UniversityCollege of Plant Protection, Northeast Agricultural UniversityPHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRDState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesLondon Research and Development Centre, Agriculture and Agri-Food CanadaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAbstract Potyvirids are the largest group of plant RNA viruses. Pelota, a core component of RNA quality controls (RQC), promotes the degradation of potyvirids’ genomic RNA by recognizing a specific G1-2A6-7 motif. Here we demonstrate that the viral RNA-dependent RNA polymerase, NIb, acts as a SUMOylation decoy to effectively reduce Pelota SUMOylation by competing with SCE1 to inhibit Pelota-mediated RQC. TuMV NIb is comprised of two functional SUMO interacting motif (SIM): SIM2 and SIM3. The former is identified as the key site for NIb’s SUMOylation by SUMO3, whereas the latter is responsible for the interaction with SCE1. These two SIMs are conserved among the majority of potyvirids-encoded NIbs. Thus, virus protein-mediated SUMOylation decoy strategy to suppress host defense may be a common feature in plant virus pathosystems. These findings highlight a dynamic interplay between plant defense mechanism and viral counter-strategy by orchestrating the post-translational modifications of virus and host defense components.https://doi.org/10.1038/s41467-024-55288-6 |
| spellingShingle | Linhao Ge Mingxuan Jia Hongying Shan Weifang Gao Lu Jiang Hongguang Cui Xiaofei Cheng Marilyne Uzest Xueping Zhou Aiming Wang Fangfang Li Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection Nature Communications |
| title | Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection |
| title_full | Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection |
| title_fullStr | Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection |
| title_full_unstemmed | Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection |
| title_short | Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection |
| title_sort | viral rna polymerase as a sumoylation decoy inhibits rna quality control to promote potyvirus infection |
| url | https://doi.org/10.1038/s41467-024-55288-6 |
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