Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber
Leucine-Rich Repeats Extensins (LRX) are a type of cell wall protein that participate in the formation of the plant cell wall and play a crucial role in plant growth and development. However, the study of LRX genes in Cucurbitaceae has not been reported. Here, 40 LRX genes were identified from seven...
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Maximum Academic Press
2024-01-01
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| Series: | Vegetable Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/vegres-0024-0025 |
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| author | Shanshan Fan Songlin Yang Kexin Shi Lin Yang Menghang An Fang Wang Yu Qi Min Feng Mingqi Wang Peixiang Geng Xingwang Liu Huazhong Ren |
| author_facet | Shanshan Fan Songlin Yang Kexin Shi Lin Yang Menghang An Fang Wang Yu Qi Min Feng Mingqi Wang Peixiang Geng Xingwang Liu Huazhong Ren |
| author_sort | Shanshan Fan |
| collection | DOAJ |
| description | Leucine-Rich Repeats Extensins (LRX) are a type of cell wall protein that participate in the formation of the plant cell wall and play a crucial role in plant growth and development. However, the study of LRX genes in Cucurbitaceae has not been reported. Here, 40 LRX genes were identified from seven cucurbit species in the Cucurbitaceae database, including cucumber (Cucumis sativus), wax gourd (Benincasa hispida), watermelon (Citrullus lanatus), pumpkin (Cucurbita maxima), moschata pumpkin (Cucurbita moschata), bottle gourd (Lagenaria siceraria), and melon (Cucumis melon). These LRX genes were divided into two subfamilies: LRX, controlling vegetative growth, and PEX, controlling reproductive growth. The gene structure, domain, and motif were relatively conserved, indicating that genes within each subfamily have similar functions. The differences in the number of LRX genes among the seven cucurbit species indicate the presence of gene loss or duplication events during evolution. Analysis of cis-regulatory elements showed that these LRX genes may be involved in plant growth and development, phytohormone response, and biotic and abiotic stress responses. In addition, the expression pattern of CsLRX genes in different tissues of cucumber and its expression analysis under salt stress and drought stress were detected by real-time quantitative PCR (qRT-PCR). The results showed that CsLRX genes showed organ-specific expression pattern in cucumber and responded to adversity stress. In summary, the results of the study provide a reference for further understanding the role of these genes in cell wall formation and the growth and development of Cucurbitaceae crops. |
| format | Article |
| id | doaj-art-e307abb9a09d48e8b4d491cbe32f8ef0 |
| institution | OA Journals |
| issn | 2769-0520 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Vegetable Research |
| spelling | doaj-art-e307abb9a09d48e8b4d491cbe32f8ef02025-08-20T02:12:19ZengMaximum Academic PressVegetable Research2769-05202024-01-014111410.48130/vegres-0024-0025vegres-0024-0025Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumberShanshan Fan0Songlin Yang1Kexin Shi2Lin Yang3Menghang An4Fang Wang5Yu Qi6Min Feng7Mingqi Wang8Peixiang Geng9Xingwang Liu10Huazhong Ren11Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Huiwen Middle School, No.6 Peixin Street, Dongcheng District, Beijing 100061, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaLeucine-Rich Repeats Extensins (LRX) are a type of cell wall protein that participate in the formation of the plant cell wall and play a crucial role in plant growth and development. However, the study of LRX genes in Cucurbitaceae has not been reported. Here, 40 LRX genes were identified from seven cucurbit species in the Cucurbitaceae database, including cucumber (Cucumis sativus), wax gourd (Benincasa hispida), watermelon (Citrullus lanatus), pumpkin (Cucurbita maxima), moschata pumpkin (Cucurbita moschata), bottle gourd (Lagenaria siceraria), and melon (Cucumis melon). These LRX genes were divided into two subfamilies: LRX, controlling vegetative growth, and PEX, controlling reproductive growth. The gene structure, domain, and motif were relatively conserved, indicating that genes within each subfamily have similar functions. The differences in the number of LRX genes among the seven cucurbit species indicate the presence of gene loss or duplication events during evolution. Analysis of cis-regulatory elements showed that these LRX genes may be involved in plant growth and development, phytohormone response, and biotic and abiotic stress responses. In addition, the expression pattern of CsLRX genes in different tissues of cucumber and its expression analysis under salt stress and drought stress were detected by real-time quantitative PCR (qRT-PCR). The results showed that CsLRX genes showed organ-specific expression pattern in cucumber and responded to adversity stress. In summary, the results of the study provide a reference for further understanding the role of these genes in cell wall formation and the growth and development of Cucurbitaceae crops.https://www.maxapress.com/article/doi/10.48130/vegres-0024-0025cucurbitaceaelrx genescell wall proteinexpression patternscucumber |
| spellingShingle | Shanshan Fan Songlin Yang Kexin Shi Lin Yang Menghang An Fang Wang Yu Qi Min Feng Mingqi Wang Peixiang Geng Xingwang Liu Huazhong Ren Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber Vegetable Research cucurbitaceae lrx genes cell wall protein expression patterns cucumber |
| title | Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| title_full | Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| title_fullStr | Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| title_full_unstemmed | Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| title_short | Genome-wide identification of the LRX gene family in Cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| title_sort | genome wide identification of the lrx gene family in cucurbitaceae and expression analysis under salt and drought stress in cucumber |
| topic | cucurbitaceae lrx genes cell wall protein expression patterns cucumber |
| url | https://www.maxapress.com/article/doi/10.48130/vegres-0024-0025 |
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