Mediator subunit 12 is required for neutrophil development in zebrafish.
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptiona...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023845&type=printable |
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| _version_ | 1850066957978566656 |
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| author | Maria-Cristina Keightley Judith E Layton John W Hayman Joan K Heath Graham J Lieschke |
| author_facet | Maria-Cristina Keightley Judith E Layton John W Hayman Joan K Heath Graham J Lieschke |
| author_sort | Maria-Cristina Keightley |
| collection | DOAJ |
| description | Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator. |
| format | Article |
| id | doaj-art-e3049a45bf784fce84b80aff8f00f856 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e3049a45bf784fce84b80aff8f00f8562025-08-20T02:48:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2384510.1371/journal.pone.0023845Mediator subunit 12 is required for neutrophil development in zebrafish.Maria-Cristina KeightleyJudith E LaytonJohn W HaymanJoan K HeathGraham J LieschkeHematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023845&type=printable |
| spellingShingle | Maria-Cristina Keightley Judith E Layton John W Hayman Joan K Heath Graham J Lieschke Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS ONE |
| title | Mediator subunit 12 is required for neutrophil development in zebrafish. |
| title_full | Mediator subunit 12 is required for neutrophil development in zebrafish. |
| title_fullStr | Mediator subunit 12 is required for neutrophil development in zebrafish. |
| title_full_unstemmed | Mediator subunit 12 is required for neutrophil development in zebrafish. |
| title_short | Mediator subunit 12 is required for neutrophil development in zebrafish. |
| title_sort | mediator subunit 12 is required for neutrophil development in zebrafish |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0023845&type=printable |
| work_keys_str_mv | AT mariacristinakeightley mediatorsubunit12isrequiredforneutrophildevelopmentinzebrafish AT judithelayton mediatorsubunit12isrequiredforneutrophildevelopmentinzebrafish AT johnwhayman mediatorsubunit12isrequiredforneutrophildevelopmentinzebrafish AT joankheath mediatorsubunit12isrequiredforneutrophildevelopmentinzebrafish AT grahamjlieschke mediatorsubunit12isrequiredforneutrophildevelopmentinzebrafish |