Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi
Radix Pueraria lobata has been recognized as a medicine and functional food, whose polysaccharide is its main bioactive component. In this study, we employed the response surface method to optimize the extraction of a polysaccharide from Radix Puerariae lobatae (PLP). Based on a single-factor experi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2024.2440028 |
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| author | Jingjing Fang Yijun Zhang Yuqing Liu Wen Cao Qiuyue Lv Jiangping Wu Guodong Wang Jun Han |
| author_facet | Jingjing Fang Yijun Zhang Yuqing Liu Wen Cao Qiuyue Lv Jiangping Wu Guodong Wang Jun Han |
| author_sort | Jingjing Fang |
| collection | DOAJ |
| description | Radix Pueraria lobata has been recognized as a medicine and functional food, whose polysaccharide is its main bioactive component. In this study, we employed the response surface method to optimize the extraction of a polysaccharide from Radix Puerariae lobatae (PLP). Based on a single-factor experiment, three levels of liquid–material ratio, extraction time, and extraction temperature were selected for the response surface experiment. We established a quadratic regression equation of the extraction rate. The optimal combination conditions were obtained through regression analysis and ridge analysis. The optimal extraction yield of PLP was up to 5.526%, which was not significantly different from the verified value. Biologically, PLP1, a homogeneous P. lobatae polysaccharide isolated and purified from PLP, exhibited significant antioxidant and hypoglycemic properties in vitro. These findings provide an important foundation for the development and utilization of P. lobatae resources. |
| format | Article |
| id | doaj-art-e303ad0af21d4df8a3dc70fcf412ef4e |
| institution | OA Journals |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-e303ad0af21d4df8a3dc70fcf412ef4e2025-08-20T01:58:41ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452024-12-0122110.1080/19476337.2024.2440028Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) OhwiJingjing Fang0Yijun Zhang1Yuqing Liu2Wen Cao3Qiuyue Lv4Jiangping Wu5Guodong Wang6Jun Han7Center for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaCenter for Xin’an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu, ChinaRadix Pueraria lobata has been recognized as a medicine and functional food, whose polysaccharide is its main bioactive component. In this study, we employed the response surface method to optimize the extraction of a polysaccharide from Radix Puerariae lobatae (PLP). Based on a single-factor experiment, three levels of liquid–material ratio, extraction time, and extraction temperature were selected for the response surface experiment. We established a quadratic regression equation of the extraction rate. The optimal combination conditions were obtained through regression analysis and ridge analysis. The optimal extraction yield of PLP was up to 5.526%, which was not significantly different from the verified value. Biologically, PLP1, a homogeneous P. lobatae polysaccharide isolated and purified from PLP, exhibited significant antioxidant and hypoglycemic properties in vitro. These findings provide an important foundation for the development and utilization of P. lobatae resources.https://www.tandfonline.com/doi/10.1080/19476337.2024.2440028Radix puerariae lobataePolysaccharideresponse surface method |
| spellingShingle | Jingjing Fang Yijun Zhang Yuqing Liu Wen Cao Qiuyue Lv Jiangping Wu Guodong Wang Jun Han Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi CyTA - Journal of Food Radix puerariae lobatae Polysaccharide response surface method |
| title | Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi |
| title_full | Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi |
| title_fullStr | Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi |
| title_full_unstemmed | Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi |
| title_short | Optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of Radix Puerariae lobatae (Willd.) Ohwi |
| title_sort | optimal extraction of a polysaccharide with antioxidant and hypoglycemic activities from the root of radix puerariae lobatae willd ohwi |
| topic | Radix puerariae lobatae Polysaccharide response surface method |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2024.2440028 |
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