Genomic and functional determinants of host spectrum in Group B Streptococcus.

Group B Streptococcus (GBS) is a major human and animal pathogen that threatens public health and food security. Spill-over and spill-back between host species is possible due to adaptation and amplification of GBS in new niches but the evolutionary and functional mechanisms underpinning those pheno...

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Main Authors: Chiara Crestani, Taya L Forde, John Bell, Samantha J Lycett, Laura M A Oliveira, Tatiana C A Pinto, Claudia G Cobo-Ángel, Alejandro Ceballos-Márquez, Nguyen N Phuoc, Wanna Sirimanapong, Swaine L Chen, Dorota Jamrozy, Stephen D Bentley, Michael Fontaine, Ruth N Zadoks
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-08-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1012400
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author Chiara Crestani
Taya L Forde
John Bell
Samantha J Lycett
Laura M A Oliveira
Tatiana C A Pinto
Claudia G Cobo-Ángel
Alejandro Ceballos-Márquez
Nguyen N Phuoc
Wanna Sirimanapong
Swaine L Chen
Dorota Jamrozy
Stephen D Bentley
Michael Fontaine
Ruth N Zadoks
author_facet Chiara Crestani
Taya L Forde
John Bell
Samantha J Lycett
Laura M A Oliveira
Tatiana C A Pinto
Claudia G Cobo-Ángel
Alejandro Ceballos-Márquez
Nguyen N Phuoc
Wanna Sirimanapong
Swaine L Chen
Dorota Jamrozy
Stephen D Bentley
Michael Fontaine
Ruth N Zadoks
author_sort Chiara Crestani
collection DOAJ
description Group B Streptococcus (GBS) is a major human and animal pathogen that threatens public health and food security. Spill-over and spill-back between host species is possible due to adaptation and amplification of GBS in new niches but the evolutionary and functional mechanisms underpinning those phenomena are poorly known. Based on analysis of 1,254 curated genomes from all major GBS host species and six continents, we found that the global GBS population comprises host-generalist, host-adapted and host-restricted sublineages, which are found across host groups, preferentially within one host group, or exclusively within one host group, respectively, and show distinct levels of recombination. Strikingly, the association of GBS genomes with the three major host groups (humans, cattle, fish) is driven by a single accessory gene cluster per host, regardless of sublineage or the breadth of host spectrum. Moreover, those gene clusters are shared with other streptococcal species occupying the same niche and are functionally relevant for host tropism. Our findings demonstrate (1) the heterogeneity of genome plasticity within a bacterial species of public health importance, enabling the identification of high-risk clones; (2) the contribution of inter-species gene transmission to the evolution of GBS; and (3) the importance of considering the role of animal hosts, and the accessory gene pool associated with their microbiota, in the evolution of multi-host bacterial pathogens. Collectively, these phenomena may explain the adaptation and clonal expansion of GBS in animal reservoirs and the risk of spill-over and spill-back between animals and humans.
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spelling doaj-art-e2fc41f843e7473a893b30472a98b2eb2025-08-20T02:38:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742024-08-01208e101240010.1371/journal.ppat.1012400Genomic and functional determinants of host spectrum in Group B Streptococcus.Chiara CrestaniTaya L FordeJohn BellSamantha J LycettLaura M A OliveiraTatiana C A PintoClaudia G Cobo-ÁngelAlejandro Ceballos-MárquezNguyen N PhuocWanna SirimanapongSwaine L ChenDorota JamrozyStephen D BentleyMichael FontaineRuth N ZadoksGroup B Streptococcus (GBS) is a major human and animal pathogen that threatens public health and food security. Spill-over and spill-back between host species is possible due to adaptation and amplification of GBS in new niches but the evolutionary and functional mechanisms underpinning those phenomena are poorly known. Based on analysis of 1,254 curated genomes from all major GBS host species and six continents, we found that the global GBS population comprises host-generalist, host-adapted and host-restricted sublineages, which are found across host groups, preferentially within one host group, or exclusively within one host group, respectively, and show distinct levels of recombination. Strikingly, the association of GBS genomes with the three major host groups (humans, cattle, fish) is driven by a single accessory gene cluster per host, regardless of sublineage or the breadth of host spectrum. Moreover, those gene clusters are shared with other streptococcal species occupying the same niche and are functionally relevant for host tropism. Our findings demonstrate (1) the heterogeneity of genome plasticity within a bacterial species of public health importance, enabling the identification of high-risk clones; (2) the contribution of inter-species gene transmission to the evolution of GBS; and (3) the importance of considering the role of animal hosts, and the accessory gene pool associated with their microbiota, in the evolution of multi-host bacterial pathogens. Collectively, these phenomena may explain the adaptation and clonal expansion of GBS in animal reservoirs and the risk of spill-over and spill-back between animals and humans.https://doi.org/10.1371/journal.ppat.1012400
spellingShingle Chiara Crestani
Taya L Forde
John Bell
Samantha J Lycett
Laura M A Oliveira
Tatiana C A Pinto
Claudia G Cobo-Ángel
Alejandro Ceballos-Márquez
Nguyen N Phuoc
Wanna Sirimanapong
Swaine L Chen
Dorota Jamrozy
Stephen D Bentley
Michael Fontaine
Ruth N Zadoks
Genomic and functional determinants of host spectrum in Group B Streptococcus.
PLoS Pathogens
title Genomic and functional determinants of host spectrum in Group B Streptococcus.
title_full Genomic and functional determinants of host spectrum in Group B Streptococcus.
title_fullStr Genomic and functional determinants of host spectrum in Group B Streptococcus.
title_full_unstemmed Genomic and functional determinants of host spectrum in Group B Streptococcus.
title_short Genomic and functional determinants of host spectrum in Group B Streptococcus.
title_sort genomic and functional determinants of host spectrum in group b streptococcus
url https://doi.org/10.1371/journal.ppat.1012400
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