Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish
This study optimized microwave-assisted extraction conditions for determining eight arsenic species in marine fish using High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry. Response surface methodology was applied to optimize four key parameters: methanol volume frac...
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| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2025.2520268 |
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| author | Xiao-qiong Wu Guo-Ying Zhu Zhong-wen Chen Miao-hua Ge |
| author_facet | Xiao-qiong Wu Guo-Ying Zhu Zhong-wen Chen Miao-hua Ge |
| author_sort | Xiao-qiong Wu |
| collection | DOAJ |
| description | This study optimized microwave-assisted extraction conditions for determining eight arsenic species in marine fish using High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry. Response surface methodology was applied to optimize four key parameters: methanol volume fraction, extraction time, extraction temperature, and solid–liquid ratio. A second-order polynomial regression model was developed using single-factor experiments and Box–Behnken design, and the accuracy of the predictive conditions was verified in six types of marine fish samples. The optimized extraction conditions were: 30% methanol, 32 minutes extraction time, 85 °C extraction temperature, and a solid–liquid ratio of 1:20 (g/mL). Under these conditions, the predicted arsenic speciation content in saury was 277.05 µg/kg, with a measured value of 297.43 µg/kg (6.90% deviation), and spiked recovery rates ranging from 82.1% to 103.2%. The total arsenic speciation in six marine fish species ranged from 300.69 to 2,541.84 µg/kg, with extraction efficiencies between 85.4% and 92.4%. The optimized extraction method significantly improved detection efficiency compared to traditional ultrasonic extraction techniques, demonstrating its suitability for arsenic speciation analysis in marine fish and contributing to a better understanding of arsenic distribution in marine food products. |
| format | Article |
| id | doaj-art-e2f964c08fd54ee7a385cd7cb31dc1b1 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj-art-e2f964c08fd54ee7a385cd7cb31dc1b12025-08-20T03:28:41ZengTaylor & Francis GroupAll Life2689-53072025-12-0118110.1080/26895293.2025.25202682520268Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fishXiao-qiong Wu0Guo-Ying Zhu1Zhong-wen Chen2Miao-hua Ge3Jiaxing Center for Disease Control and Prevention, Jiaxing, Zhejiang Province, People’s Republic of ChinaJiaxing Center for Disease Control and Prevention, Jiaxing, Zhejiang Province, People’s Republic of ChinaJiaxing Center for Disease Control and Prevention, Jiaxing, Zhejiang Province, People’s Republic of ChinaJiaxing Center for Disease Control and Prevention, Jiaxing, Zhejiang Province, People’s Republic of ChinaThis study optimized microwave-assisted extraction conditions for determining eight arsenic species in marine fish using High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry. Response surface methodology was applied to optimize four key parameters: methanol volume fraction, extraction time, extraction temperature, and solid–liquid ratio. A second-order polynomial regression model was developed using single-factor experiments and Box–Behnken design, and the accuracy of the predictive conditions was verified in six types of marine fish samples. The optimized extraction conditions were: 30% methanol, 32 minutes extraction time, 85 °C extraction temperature, and a solid–liquid ratio of 1:20 (g/mL). Under these conditions, the predicted arsenic speciation content in saury was 277.05 µg/kg, with a measured value of 297.43 µg/kg (6.90% deviation), and spiked recovery rates ranging from 82.1% to 103.2%. The total arsenic speciation in six marine fish species ranged from 300.69 to 2,541.84 µg/kg, with extraction efficiencies between 85.4% and 92.4%. The optimized extraction method significantly improved detection efficiency compared to traditional ultrasonic extraction techniques, demonstrating its suitability for arsenic speciation analysis in marine fish and contributing to a better understanding of arsenic distribution in marine food products.http://dx.doi.org/10.1080/26895293.2025.2520268arsenic speciationhigh performance liquid chromatography-inductively coupled plasma mass spectrometrymarine fishresponse surface methodology |
| spellingShingle | Xiao-qiong Wu Guo-Ying Zhu Zhong-wen Chen Miao-hua Ge Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish All Life arsenic speciation high performance liquid chromatography-inductively coupled plasma mass spectrometry marine fish response surface methodology |
| title | Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| title_full | Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| title_fullStr | Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| title_full_unstemmed | Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| title_short | Response surface optimization of microwave-assisted high performance liquid chromatography-inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| title_sort | response surface optimization of microwave assisted high performance liquid chromatography inductively coupled plasma mass spectrometry for determining eight arsenic species in marine fish |
| topic | arsenic speciation high performance liquid chromatography-inductively coupled plasma mass spectrometry marine fish response surface methodology |
| url | http://dx.doi.org/10.1080/26895293.2025.2520268 |
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