SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells
Summary: m6A RNA methylation is the most prevalent internal modification in mammalian mRNAs and is involved in many biological processes. METTL16 is a recently identified RNA m6A methyltransferase. However, how METTL16 activity is regulated remains poorly understood. Here, we report a previously unr...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725006977 |
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| author | Haixia Li Emma Wilkinson Yan-Hong Cui Ming Sun Kenneth Lu Seungwon Yang Marc Bissonnette Yu-Ying He |
| author_facet | Haixia Li Emma Wilkinson Yan-Hong Cui Ming Sun Kenneth Lu Seungwon Yang Marc Bissonnette Yu-Ying He |
| author_sort | Haixia Li |
| collection | DOAJ |
| description | Summary: m6A RNA methylation is the most prevalent internal modification in mammalian mRNAs and is involved in many biological processes. METTL16 is a recently identified RNA m6A methyltransferase. However, how METTL16 activity is regulated remains poorly understood. Here, we report a previously unrecognized mechanism in regulating METTL16 activity. SSB not only serves as a co-factor for METTL16 in installing m6A RNA methylation by enhancing METTL16 binding to RNA but it also is a direct target of METTL16-mediated m6A RNA methylation, leading to a positive auto-regulatory loop that promotes m6A methylation, SSB expression, and chemoresistance in colorectal cancer cells. Our findings reveal the regulation of METTL16 activity by SSB, providing a basis for the development of future therapeutic strategies that target the METTL16/SSB axis in METTL16-dependent cancers such as colorectal cancer. |
| format | Article |
| id | doaj-art-e2f3bdfce414484f84f418dd1b25dbb4 |
| institution | OA Journals |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-e2f3bdfce414484f84f418dd1b25dbb42025-08-20T02:35:22ZengElsevierCell Reports2211-12472025-07-0144711592610.1016/j.celrep.2025.115926SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cellsHaixia Li0Emma Wilkinson1Yan-Hong Cui2Ming Sun3Kenneth Lu4Seungwon Yang5Marc Bissonnette6Yu-Ying He7Department of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Gastroenterology, Hepatology & Nutrition, University of Chicago, Chicago, IL, USADepartment of Medicine, Section of Dermatology, University of Chicago, Chicago, IL, USA; Corresponding authorSummary: m6A RNA methylation is the most prevalent internal modification in mammalian mRNAs and is involved in many biological processes. METTL16 is a recently identified RNA m6A methyltransferase. However, how METTL16 activity is regulated remains poorly understood. Here, we report a previously unrecognized mechanism in regulating METTL16 activity. SSB not only serves as a co-factor for METTL16 in installing m6A RNA methylation by enhancing METTL16 binding to RNA but it also is a direct target of METTL16-mediated m6A RNA methylation, leading to a positive auto-regulatory loop that promotes m6A methylation, SSB expression, and chemoresistance in colorectal cancer cells. Our findings reveal the regulation of METTL16 activity by SSB, providing a basis for the development of future therapeutic strategies that target the METTL16/SSB axis in METTL16-dependent cancers such as colorectal cancer.http://www.sciencedirect.com/science/article/pii/S2211124725006977CP: CancerCP: Molecular biology |
| spellingShingle | Haixia Li Emma Wilkinson Yan-Hong Cui Ming Sun Kenneth Lu Seungwon Yang Marc Bissonnette Yu-Ying He SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells Cell Reports CP: Cancer CP: Molecular biology |
| title | SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells |
| title_full | SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells |
| title_fullStr | SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells |
| title_full_unstemmed | SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells |
| title_short | SSB cooperates with METTL16-mediated m6A RNA methylation to promote chemoresistance in colorectal cancer cells |
| title_sort | ssb cooperates with mettl16 mediated m6a rna methylation to promote chemoresistance in colorectal cancer cells |
| topic | CP: Cancer CP: Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725006977 |
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