Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa
This study aimed to evaluate the effects of resveratrol (RES) supplementation in the cryopreservation medium on the conventional and non-conventional quality parameters, oxidative status, and microbial profile of cryopreserved turkey semen. Ejaculates (n = 40) were cryopreserved in a modified Beltsv...
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Sciendo
2025-06-01
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| Series: | Contemporary Agriculture |
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| Online Access: | https://doi.org/10.2478/contagri-2025-0009 |
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| author | Tvrdá Eva Ďuračka Michal Kačániová Miroslava Slanina Tomáš |
| author_facet | Tvrdá Eva Ďuračka Michal Kačániová Miroslava Slanina Tomáš |
| author_sort | Tvrdá Eva |
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| description | This study aimed to evaluate the effects of resveratrol (RES) supplementation in the cryopreservation medium on the conventional and non-conventional quality parameters, oxidative status, and microbial profile of cryopreserved turkey semen. Ejaculates (n = 40) were cryopreserved in a modified Beltsville extender either without RES (the cryopreserved control [CtrlC]) or with 5, 10, or 25 µM RES . Fresh semen served as a negative control (CtrlN). Post-thaw analyses included assessments of motility, viability, membrane functionality, mitochondrial activity, DNA fragmentation, apoptotic status, reactive oxygen species (ROS), protein carbonyl (PC), and malondialdehyde (MDA) levels. Bacteriological analysis was performed using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results obtained indicate that the sperm quality, particularly the mitochondrial activity, was significantly improved following the administration of 5 μM RES compared to CtrlC (p < 0.05). All RES doses were particularly effectvive in preventing the ROS overgeneration and associated lipid peroxidation relative to CtrlC (p<0.05). The bacterial load decreased in a dose-dependent manner, whereas RES was found effective in enhancing the antibacterial efficacy of gentamicin in the frozen-thawed semen. In conclusion, this study demonstrates that supplementing the modified Beltsville extender with 5 or 10 µM RES improves post-thaw turkey semen quality. |
| format | Article |
| id | doaj-art-e2f2bb35b6544c1393cae48a74ace1cb |
| institution | OA Journals |
| issn | 2466-4774 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sciendo |
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| series | Contemporary Agriculture |
| spelling | doaj-art-e2f2bb35b6544c1393cae48a74ace1cb2025-08-20T02:36:53ZengSciendoContemporary Agriculture2466-47742025-06-01741-2647410.2478/contagri-2025-0009Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey SpermatozoaTvrdá Eva0Ďuračka Michal1Kačániová Miroslava2Slanina Tomáš31Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76Nitra, Slovakia2Agrobiotech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76Nitra, Slovakia3Institute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76Nitra, Slovakia5Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76Nitra, SlovakiaThis study aimed to evaluate the effects of resveratrol (RES) supplementation in the cryopreservation medium on the conventional and non-conventional quality parameters, oxidative status, and microbial profile of cryopreserved turkey semen. Ejaculates (n = 40) were cryopreserved in a modified Beltsville extender either without RES (the cryopreserved control [CtrlC]) or with 5, 10, or 25 µM RES . Fresh semen served as a negative control (CtrlN). Post-thaw analyses included assessments of motility, viability, membrane functionality, mitochondrial activity, DNA fragmentation, apoptotic status, reactive oxygen species (ROS), protein carbonyl (PC), and malondialdehyde (MDA) levels. Bacteriological analysis was performed using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results obtained indicate that the sperm quality, particularly the mitochondrial activity, was significantly improved following the administration of 5 μM RES compared to CtrlC (p < 0.05). All RES doses were particularly effectvive in preventing the ROS overgeneration and associated lipid peroxidation relative to CtrlC (p<0.05). The bacterial load decreased in a dose-dependent manner, whereas RES was found effective in enhancing the antibacterial efficacy of gentamicin in the frozen-thawed semen. In conclusion, this study demonstrates that supplementing the modified Beltsville extender with 5 or 10 µM RES improves post-thaw turkey semen quality.https://doi.org/10.2478/contagri-2025-0009antioxidantcryopreservationsemenspermatozoaturkeyresveratrol |
| spellingShingle | Tvrdá Eva Ďuračka Michal Kačániová Miroslava Slanina Tomáš Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa Contemporary Agriculture antioxidant cryopreservation semen spermatozoa turkey resveratrol |
| title | Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa |
| title_full | Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa |
| title_fullStr | Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa |
| title_full_unstemmed | Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa |
| title_short | Cryoprotective and Antibacterial Effects of Resveratrol on Frozen-Thawed Turkey Spermatozoa |
| title_sort | cryoprotective and antibacterial effects of resveratrol on frozen thawed turkey spermatozoa |
| topic | antioxidant cryopreservation semen spermatozoa turkey resveratrol |
| url | https://doi.org/10.2478/contagri-2025-0009 |
| work_keys_str_mv | AT tvrdaeva cryoprotectiveandantibacterialeffectsofresveratrolonfrozenthawedturkeyspermatozoa AT durackamichal cryoprotectiveandantibacterialeffectsofresveratrolonfrozenthawedturkeyspermatozoa AT kacaniovamiroslava cryoprotectiveandantibacterialeffectsofresveratrolonfrozenthawedturkeyspermatozoa AT slaninatomas cryoprotectiveandantibacterialeffectsofresveratrolonfrozenthawedturkeyspermatozoa |