Quantitative determination of the spatial distribution of components in single cells with CellDetail
Abstract The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Detection and Analysis of Intensity Lounge (CellDetail)...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54638-8 |
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| author | Tanja Schuster Amanda Amoah Angelika Vollmer Gina Marka Julian Niemann Mehmet Saçma Vadim Sakk Karin Soller Mona Vogel Ani Grigoryan Meinhard Wlaschek Karin Scharffetter-Kochanek Medhanie Mulaw Hartmut Geiger |
| author_facet | Tanja Schuster Amanda Amoah Angelika Vollmer Gina Marka Julian Niemann Mehmet Saçma Vadim Sakk Karin Soller Mona Vogel Ani Grigoryan Meinhard Wlaschek Karin Scharffetter-Kochanek Medhanie Mulaw Hartmut Geiger |
| author_sort | Tanja Schuster |
| collection | DOAJ |
| description | Abstract The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Detection and Analysis of Intensity Lounge (CellDetail). The algorithm within CellDetail is based on the concept of the dipole moment. CellDetail provides quantitative values for the distribution of the polarity proteins Cdc42 and Tubulin in young and aged hematopoietic stem cells (HSCs). Septin proteins form networks within cells that are critical for cell compartmentalization. We uncover a reduced level of organization of the Septin network within aged HSCs and within senescent human fibroblasts. Changes in the Septin network structure might therefore be a common feature of aging. The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42. |
| format | Article |
| id | doaj-art-e2f1b5bd1b014883b299f1a7e216559d |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e2f1b5bd1b014883b299f1a7e216559d2025-08-20T02:49:16ZengNature PortfolioNature Communications2041-17232024-11-0115111310.1038/s41467-024-54638-8Quantitative determination of the spatial distribution of components in single cells with CellDetailTanja Schuster0Amanda Amoah1Angelika Vollmer2Gina Marka3Julian Niemann4Mehmet Saçma5Vadim Sakk6Karin Soller7Mona Vogel8Ani Grigoryan9Meinhard Wlaschek10Karin Scharffetter-Kochanek11Medhanie Mulaw12Hartmut Geiger13Institute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityDepartment of Dermatology and Allergic Diseases, Ulm UniversityDepartment of Dermatology and Allergic Diseases, Ulm UniversityUnit for Single-Cell Genomics, Ulm UniversityInstitute of Molecular Medicine, Ulm UniversityAbstract The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Detection and Analysis of Intensity Lounge (CellDetail). The algorithm within CellDetail is based on the concept of the dipole moment. CellDetail provides quantitative values for the distribution of the polarity proteins Cdc42 and Tubulin in young and aged hematopoietic stem cells (HSCs). Septin proteins form networks within cells that are critical for cell compartmentalization. We uncover a reduced level of organization of the Septin network within aged HSCs and within senescent human fibroblasts. Changes in the Septin network structure might therefore be a common feature of aging. The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42.https://doi.org/10.1038/s41467-024-54638-8 |
| spellingShingle | Tanja Schuster Amanda Amoah Angelika Vollmer Gina Marka Julian Niemann Mehmet Saçma Vadim Sakk Karin Soller Mona Vogel Ani Grigoryan Meinhard Wlaschek Karin Scharffetter-Kochanek Medhanie Mulaw Hartmut Geiger Quantitative determination of the spatial distribution of components in single cells with CellDetail Nature Communications |
| title | Quantitative determination of the spatial distribution of components in single cells with CellDetail |
| title_full | Quantitative determination of the spatial distribution of components in single cells with CellDetail |
| title_fullStr | Quantitative determination of the spatial distribution of components in single cells with CellDetail |
| title_full_unstemmed | Quantitative determination of the spatial distribution of components in single cells with CellDetail |
| title_short | Quantitative determination of the spatial distribution of components in single cells with CellDetail |
| title_sort | quantitative determination of the spatial distribution of components in single cells with celldetail |
| url | https://doi.org/10.1038/s41467-024-54638-8 |
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