Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread

This study investigates the effect of extended rye scald fermentation times (12–48 h) on its biochemical properties and rye bread’s nutritional and sensory qualities. Traditional rye bread production in Latvia involves prolonged fermentation with lactic acid bacteria (LAB), a process that influences...

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Main Authors: Ruta Murniece, Sanita Reidzane, Vitalijs Radenkovs, Evita Straumite, Anete Keke, Eeva-Gerda Kobrin, Dace Klava
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/6/979
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author Ruta Murniece
Sanita Reidzane
Vitalijs Radenkovs
Evita Straumite
Anete Keke
Eeva-Gerda Kobrin
Dace Klava
author_facet Ruta Murniece
Sanita Reidzane
Vitalijs Radenkovs
Evita Straumite
Anete Keke
Eeva-Gerda Kobrin
Dace Klava
author_sort Ruta Murniece
collection DOAJ
description This study investigates the effect of extended rye scald fermentation times (12–48 h) on its biochemical properties and rye bread’s nutritional and sensory qualities. Traditional rye bread production in Latvia involves prolonged fermentation with lactic acid bacteria (LAB), a process that influences the bread’s acidity, sugar content, and concentrations of organic acids, fructans, and phytates. Scald fermentation was analyzed at intervals of 0, 12, 24, 36, and 48 h to monitor microbial activity, particularly LAB population dynamics. Organic acids and sugar profiles were analyzed using HPLC, while phytic acid and fructan concentrations were determined using the Phytic Acid Assay Kit (K-PHYT) and Fructan Assay Kit (K-FRUC). Sensory evaluation assessed attributes including aroma, sour and sweet taste, stickiness, and floury aftertaste. A rapid pH decrease and increased total titratable acidity (TTA) after 12 h confirmed scald’s suitability as a substrate for <i>Lactobacillus delbrueckii</i> metabolism. Lactic acid content increased 13.8-fold after 48 h. Combined scald and dough sourdough fermentation reduced phytic acid by 20% and fructans by 49%, improving mineral bioavailability. Extending fermentation beyond 24 h showed no significant differences in physicochemical parameters, although it improved sensory quality, reduced stickiness, balanced sweet–sour flavors, enhanced aroma, and minimized floury aftertaste.
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spelling doaj-art-e2e5a146c55a4f14a95c8bfbe1d752f72025-08-20T02:42:32ZengMDPI AGFoods2304-81582025-03-0114697910.3390/foods14060979Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye BreadRuta Murniece0Sanita Reidzane1Vitalijs Radenkovs2Evita Straumite3Anete Keke4Eeva-Gerda Kobrin5Dace Klava6Food Institute, Latvia University of Life Sciences and Technologies, Riga Street 22, LV-3004 Jelgava, LatviaFood Institute, Latvia University of Life Sciences and Technologies, Riga Street 22, LV-3004 Jelgava, LatviaDivision of Smart Technologies, Research Laboratory of Biotechnology, Latvia University of Life Sciences and Technologies, Rigas Street 22b, LV-3004 Jelgava, LatviaFood Institute, Latvia University of Life Sciences and Technologies, Riga Street 22, LV-3004 Jelgava, LatviaFood Institute, Latvia University of Life Sciences and Technologies, Riga Street 22, LV-3004 Jelgava, LatviaAS TFTAK (Center of Food and Fermentation Technologies), Mäealuse 2/4B, 12618 Tallinn, EstoniaFood Institute, Latvia University of Life Sciences and Technologies, Riga Street 22, LV-3004 Jelgava, LatviaThis study investigates the effect of extended rye scald fermentation times (12–48 h) on its biochemical properties and rye bread’s nutritional and sensory qualities. Traditional rye bread production in Latvia involves prolonged fermentation with lactic acid bacteria (LAB), a process that influences the bread’s acidity, sugar content, and concentrations of organic acids, fructans, and phytates. Scald fermentation was analyzed at intervals of 0, 12, 24, 36, and 48 h to monitor microbial activity, particularly LAB population dynamics. Organic acids and sugar profiles were analyzed using HPLC, while phytic acid and fructan concentrations were determined using the Phytic Acid Assay Kit (K-PHYT) and Fructan Assay Kit (K-FRUC). Sensory evaluation assessed attributes including aroma, sour and sweet taste, stickiness, and floury aftertaste. A rapid pH decrease and increased total titratable acidity (TTA) after 12 h confirmed scald’s suitability as a substrate for <i>Lactobacillus delbrueckii</i> metabolism. Lactic acid content increased 13.8-fold after 48 h. Combined scald and dough sourdough fermentation reduced phytic acid by 20% and fructans by 49%, improving mineral bioavailability. Extending fermentation beyond 24 h showed no significant differences in physicochemical parameters, although it improved sensory quality, reduced stickiness, balanced sweet–sour flavors, enhanced aroma, and minimized floury aftertaste.https://www.mdpi.com/2304-8158/14/6/979rye breadsourdoughfermentable sugarsfructansphytic acid
spellingShingle Ruta Murniece
Sanita Reidzane
Vitalijs Radenkovs
Evita Straumite
Anete Keke
Eeva-Gerda Kobrin
Dace Klava
Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
Foods
rye bread
sourdough
fermentable sugars
fructans
phytic acid
title Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
title_full Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
title_fullStr Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
title_full_unstemmed Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
title_short Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread
title_sort scald fermentation time as a factor determining the nutritional and sensory quality of rye bread
topic rye bread
sourdough
fermentable sugars
fructans
phytic acid
url https://www.mdpi.com/2304-8158/14/6/979
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