The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster

Abstract Photodegradation is a promising technique for remediating contaminated environmental matrices. It demonstrates significant potential in transforming organic contaminants into carbon dioxide, water, and inorganic anions through degradation reactions that involve transient oxidizing species,...

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Main Authors: Douglas Lisboa Ramalho, Jadyellen Rondon Silva, Maria Eduarda Monteiro Martins dos Santos, Maria Eduarda Silva Soares, Andrielle Adelina Teodoro Jesus, Thiago Henrique Oliveira Alves, Michelle Fernanda Brugnera, Sidnei Moura, Anderson Oliveira Souza
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Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-86413-0
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author Douglas Lisboa Ramalho
Jadyellen Rondon Silva
Maria Eduarda Monteiro Martins dos Santos
Maria Eduarda Silva Soares
Andrielle Adelina Teodoro Jesus
Thiago Henrique Oliveira Alves
Michelle Fernanda Brugnera
Sidnei Moura
Anderson Oliveira Souza
author_facet Douglas Lisboa Ramalho
Jadyellen Rondon Silva
Maria Eduarda Monteiro Martins dos Santos
Maria Eduarda Silva Soares
Andrielle Adelina Teodoro Jesus
Thiago Henrique Oliveira Alves
Michelle Fernanda Brugnera
Sidnei Moura
Anderson Oliveira Souza
author_sort Douglas Lisboa Ramalho
collection DOAJ
description Abstract Photodegradation is a promising technique for remediating contaminated environmental matrices. It demonstrates significant potential in transforming organic contaminants into carbon dioxide, water, and inorganic anions through degradation reactions that involve transient oxidizing species, mainly hydroxyl radicals generated by ultraviolet (UV) irradiation. In this study, we investigated whether the photodegradation of Paraquat (PQ) with UV irradiation reduced its toxicity in Drosophila melanogaster. Our results indicate that ingesting PQ degradation products by larvae resulted in a low axial ratio (pupal volume). In the adults, it resulted in markedly diminished climbing ability in a time-dependent manner after 10 days of feeding. In addition, exposure of D. melanogaster to photodegradation of PQ reduced acetylcholinesterase and citrate synthase activities but improved oxidative stress, as evidenced by protein carbonyl, and lactate production. These results suggest that the photodegradation of PQ with UV irradiation produced PQ fragments with higher toxicity than PQ, while the precise mechanism of its action requires further investigation.
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spelling doaj-art-e2e542288e2e4a10820a40dae681cbc22025-08-20T01:51:27ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-86413-0The neurotoxicity of Paraquat and its degradation products on drosophila melanogasterDouglas Lisboa Ramalho0Jadyellen Rondon Silva1Maria Eduarda Monteiro Martins dos Santos2Maria Eduarda Silva Soares3Andrielle Adelina Teodoro Jesus4Thiago Henrique Oliveira Alves5Michelle Fernanda Brugnera6Sidnei Moura7Anderson Oliveira Souza8Mitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoBiocide Residue Analysis Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoBiotechnology of Natural and Synthetic Products Laboratory, Institute of Biotechnology, University of Caxias do SulMitochondrial Metabolism and Neurotoxicology Laboratory, Department of Chemistry, Institute of Chemistry, Federal University of Mato GrossoAbstract Photodegradation is a promising technique for remediating contaminated environmental matrices. It demonstrates significant potential in transforming organic contaminants into carbon dioxide, water, and inorganic anions through degradation reactions that involve transient oxidizing species, mainly hydroxyl radicals generated by ultraviolet (UV) irradiation. In this study, we investigated whether the photodegradation of Paraquat (PQ) with UV irradiation reduced its toxicity in Drosophila melanogaster. Our results indicate that ingesting PQ degradation products by larvae resulted in a low axial ratio (pupal volume). In the adults, it resulted in markedly diminished climbing ability in a time-dependent manner after 10 days of feeding. In addition, exposure of D. melanogaster to photodegradation of PQ reduced acetylcholinesterase and citrate synthase activities but improved oxidative stress, as evidenced by protein carbonyl, and lactate production. These results suggest that the photodegradation of PQ with UV irradiation produced PQ fragments with higher toxicity than PQ, while the precise mechanism of its action requires further investigation.https://doi.org/10.1038/s41598-025-86413-0PhotodegradationDegradation productsDrosophila melanogasterUV irradiationNeurotoxicity
spellingShingle Douglas Lisboa Ramalho
Jadyellen Rondon Silva
Maria Eduarda Monteiro Martins dos Santos
Maria Eduarda Silva Soares
Andrielle Adelina Teodoro Jesus
Thiago Henrique Oliveira Alves
Michelle Fernanda Brugnera
Sidnei Moura
Anderson Oliveira Souza
The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
Scientific Reports
Photodegradation
Degradation products
Drosophila melanogaster
UV irradiation
Neurotoxicity
title The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
title_full The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
title_fullStr The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
title_full_unstemmed The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
title_short The neurotoxicity of Paraquat and its degradation products on drosophila melanogaster
title_sort neurotoxicity of paraquat and its degradation products on drosophila melanogaster
topic Photodegradation
Degradation products
Drosophila melanogaster
UV irradiation
Neurotoxicity
url https://doi.org/10.1038/s41598-025-86413-0
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