SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE
Aim. To assess the phenotypes of proinflammatory (M1) and anti-inflammatory (M2) activation of blood monocytes in type 2 diabetes (DM2) comparing to coronary heart disease patients (CHD).Material and methods. Totally, 55 CHD patients assessed, of those 28 (11M/17F) were first time diagnosed with DM2...
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«FIRMA «SILICEA» LLC
2017-12-01
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| Series: | Российский кардиологический журнал |
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| Online Access: | https://russjcardiol.elpub.ru/jour/article/view/2550 |
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| author | К. О. Galstyan L. V. Nedosugova N. G. Nikiforov К. I. Kolmychkova Т. V. Kirichenko I. A. Sobenin |
| author_facet | К. О. Galstyan L. V. Nedosugova N. G. Nikiforov К. I. Kolmychkova Т. V. Kirichenko I. A. Sobenin |
| author_sort | К. О. Galstyan |
| collection | DOAJ |
| description | Aim. To assess the phenotypes of proinflammatory (M1) and anti-inflammatory (M2) activation of blood monocytes in type 2 diabetes (DM2) comparing to coronary heart disease patients (CHD).Material and methods. Totally, 55 CHD patients assessed, of those 28 (11M/17F) were first time diagnosed with DM2 at current hospitalization (HbA1c level 9,7% SD=2,4), not taking previously any glucose lowering therapy; and 27 patients with CHD (20M, 7F), with no glucose metabolism disorders. By immune enzyme assay method (IEA) proinflammatory monocyte activation was evaluated by spontaneous and interferon gamma (IFN-ɣ) induced secretion of proinflammatory cytokine tumour necrosis factor alpha (TNF-α), and anti-inflammatory activation of monocytes by spontaneous and interleukin-4 (IL-4) induced secretion of anti-inflammatory cytokine CCL18.Results. There was found an increased ability of monocytes in DM2 patients to secrete proand anti-inflammatory cytokines comparing to the controls and CHD patients. Basal TNF-α secretion was higher than control level 2,8 times, and stimulated — 2,2 times. Values of the basal and stimulated TNF-α secretion in CHD patients were significantly lower than in controls. There was positive correlation of HbA1c level and basal secretion of TNF-α. Basal and stimulated secretion of anti-inflammatory cytokine CCL18 in DM2 patients was significantly higher than control level — 28 pg/mL (SD=3) and 1158 (SD=68) pg/mL of the cultural medium, respectively, and in CHD patients these parameters were lower than the control level — 0,26 pg/mL (SD=0,14) and 65 (SD=33) pg/mL, respectively. Conclusion. In DM2 there is disbalance of M1/M2 activation of monocytes comparing to controls and CHD, that points on overactivation by proinflammatory phenotype. |
| format | Article |
| id | doaj-art-e2df8fc953f44c5bb13f0eddbd929103 |
| institution | Kabale University |
| issn | 1560-4071 2618-7620 |
| language | Russian |
| publishDate | 2017-12-01 |
| publisher | «FIRMA «SILICEA» LLC |
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| series | Российский кардиологический журнал |
| spelling | doaj-art-e2df8fc953f44c5bb13f0eddbd9291032025-08-20T03:43:36Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202017-12-01012212510.15829/1560-4071-2017-12-21-252258SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASEК. О. Galstyan0L. V. Nedosugova1N. G. Nikiforov2К. I. Kolmychkova3Т. V. Kirichenko4I. A. Sobenin5I. M. Sechenov First Moscow State Medical University of the Ministry of HealthI. M. Sechenov First Moscow State Medical University of the Ministry of HealthScientific Medical Research Center of Cardiology of the Ministry of HealthANO SRI of Atherosclerosis of RANSANO SRI of Atherosclerosis of RANSScientific Medical Research Center of Cardiology of the Ministry of HealthAim. To assess the phenotypes of proinflammatory (M1) and anti-inflammatory (M2) activation of blood monocytes in type 2 diabetes (DM2) comparing to coronary heart disease patients (CHD).Material and methods. Totally, 55 CHD patients assessed, of those 28 (11M/17F) were first time diagnosed with DM2 at current hospitalization (HbA1c level 9,7% SD=2,4), not taking previously any glucose lowering therapy; and 27 patients with CHD (20M, 7F), with no glucose metabolism disorders. By immune enzyme assay method (IEA) proinflammatory monocyte activation was evaluated by spontaneous and interferon gamma (IFN-ɣ) induced secretion of proinflammatory cytokine tumour necrosis factor alpha (TNF-α), and anti-inflammatory activation of monocytes by spontaneous and interleukin-4 (IL-4) induced secretion of anti-inflammatory cytokine CCL18.Results. There was found an increased ability of monocytes in DM2 patients to secrete proand anti-inflammatory cytokines comparing to the controls and CHD patients. Basal TNF-α secretion was higher than control level 2,8 times, and stimulated — 2,2 times. Values of the basal and stimulated TNF-α secretion in CHD patients were significantly lower than in controls. There was positive correlation of HbA1c level and basal secretion of TNF-α. Basal and stimulated secretion of anti-inflammatory cytokine CCL18 in DM2 patients was significantly higher than control level — 28 pg/mL (SD=3) and 1158 (SD=68) pg/mL of the cultural medium, respectively, and in CHD patients these parameters were lower than the control level — 0,26 pg/mL (SD=0,14) and 65 (SD=33) pg/mL, respectively. Conclusion. In DM2 there is disbalance of M1/M2 activation of monocytes comparing to controls and CHD, that points on overactivation by proinflammatory phenotype.https://russjcardiol.elpub.ru/jour/article/view/2550type 2 diabetesatherosclerosischdm1/m2 monocyte activationinflammationoxidative stress |
| spellingShingle | К. О. Galstyan L. V. Nedosugova N. G. Nikiforov К. I. Kolmychkova Т. V. Kirichenko I. A. Sobenin SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE Российский кардиологический журнал type 2 diabetes atherosclerosis chd m1/m2 monocyte activation inflammation oxidative stress |
| title | SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE |
| title_full | SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE |
| title_fullStr | SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE |
| title_full_unstemmed | SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE |
| title_short | SIGNIFICANCE OF M1 AND M2 POLARIZATION OF MONOCYTES-MACROPHAGES IN THE BLOOD FOR ATHEROSCLEROSIS RISK ASSESSMENT IN TYPE 2 DIABETES COMPARING WITH CORONARY HEART DISEASE |
| title_sort | significance of m1 and m2 polarization of monocytes macrophages in the blood for atherosclerosis risk assessment in type 2 diabetes comparing with coronary heart disease |
| topic | type 2 diabetes atherosclerosis chd m1/m2 monocyte activation inflammation oxidative stress |
| url | https://russjcardiol.elpub.ru/jour/article/view/2550 |
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