Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment
Soil salinity is a crucial challenge that restricts plant development and productivity. In this study, we aimed to report a promising marine-derived plant growth promoting (PGP) bacteria that can empower wheat plants to survive upon salt stress. Thirty-one bacterial isolates derived from marine envi...
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2025-07-01
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| Series: | Novel Research in Microbiology Journal |
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| author | Eman Hamada Adel Eltoukhy Metwally K. Mahfouz Abdel Kareem S.H. Mohamed |
| author_facet | Eman Hamada Adel Eltoukhy Metwally K. Mahfouz Abdel Kareem S.H. Mohamed |
| author_sort | Eman Hamada |
| collection | DOAJ |
| description | Soil salinity is a crucial challenge that restricts plant development and productivity. In this study, we aimed to report a promising marine-derived plant growth promoting (PGP) bacteria that can empower wheat plants to survive upon salt stress. Thirty-one bacterial isolates derived from marine environments were isolated and assessed for PGP activities. Among these tested isolates, a strain coded as AE-EH1 showed promising PGP activities. Based on the phenotypic characterization, using 16S rRNA gene sequencing, phylogenetic, and biochemical analyses, the selected isolate was identified as Bacillus sp. strain AE-EH1 and assigned an accession no. of OR144427. The target strain AE-EH1 could produce indole acetic acid, ammonia, and HCN, furthermore, it could fix atmospheric nitrogen and solubilize phosphate. Meanwhile, the AE-EH1 was characterized as a halotolerant bacterium with a tolerance limit of up to 15 % NaCl with optimum growth at 3 % NaCl. Interestingly, AE-EH1 showed a high in vitro antagonistic effect against 6 common plant pathogenic fungal species, including Botrytis fabae, B. cinerea, Fusarium oxysporum, Macrophomina phaseolina, Rhizoctonia solani, and Sclerotinia sclerotiorum. Inoculating, AE-EH1 into wheat cvs. Sakha 95 and Masr 3 under salt stress markedly improved the salt tolerance of these wheat cultivars. Moreover, fresh and dry weights of wheat cultivars had improved compared to non-treated plants as well as chlorophyll a, b, and carotenoids. Total carbohydrates, proteins, and lipids were increased in both Sakha 95 and Masr 3 cultivars as an influence of bacterial strain inoculation. Antioxidant peroxidase, catalase, and proline contents were accumulated in both wheat varieties. Our study provides a promising bacterial strain that can alleviate salt stress and enhance the growth and productivity of wheat under stress and normal conditions. |
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| id | doaj-art-e2d998d7f6d1404c8f8f04605f78b810 |
| institution | Kabale University |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | ResearchersLinks, Ltd |
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| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-e2d998d7f6d1404c8f8f04605f78b8102025-08-20T03:56:05ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942025-07-0194289308https://dx.doi.org/10.17582/journal.NRMJ/2025/9.4.289.308Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine EnvironmentEman Hamada0Adel Eltoukhy1Metwally K. Mahfouz2Abdel Kareem S.H. Mohamed3Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, EgyptDepartment of Biochemistry, Animal Health Research Institute, Sohag Branch, Agriculture Research Center, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, EgyptSoil salinity is a crucial challenge that restricts plant development and productivity. In this study, we aimed to report a promising marine-derived plant growth promoting (PGP) bacteria that can empower wheat plants to survive upon salt stress. Thirty-one bacterial isolates derived from marine environments were isolated and assessed for PGP activities. Among these tested isolates, a strain coded as AE-EH1 showed promising PGP activities. Based on the phenotypic characterization, using 16S rRNA gene sequencing, phylogenetic, and biochemical analyses, the selected isolate was identified as Bacillus sp. strain AE-EH1 and assigned an accession no. of OR144427. The target strain AE-EH1 could produce indole acetic acid, ammonia, and HCN, furthermore, it could fix atmospheric nitrogen and solubilize phosphate. Meanwhile, the AE-EH1 was characterized as a halotolerant bacterium with a tolerance limit of up to 15 % NaCl with optimum growth at 3 % NaCl. Interestingly, AE-EH1 showed a high in vitro antagonistic effect against 6 common plant pathogenic fungal species, including Botrytis fabae, B. cinerea, Fusarium oxysporum, Macrophomina phaseolina, Rhizoctonia solani, and Sclerotinia sclerotiorum. Inoculating, AE-EH1 into wheat cvs. Sakha 95 and Masr 3 under salt stress markedly improved the salt tolerance of these wheat cultivars. Moreover, fresh and dry weights of wheat cultivars had improved compared to non-treated plants as well as chlorophyll a, b, and carotenoids. Total carbohydrates, proteins, and lipids were increased in both Sakha 95 and Masr 3 cultivars as an influence of bacterial strain inoculation. Antioxidant peroxidase, catalase, and proline contents were accumulated in both wheat varieties. Our study provides a promising bacterial strain that can alleviate salt stress and enhance the growth and productivity of wheat under stress and normal conditions.https://researcherslinks.com/current-issues/Mitigation-of-Salt-Stress-in-Two-Wheat/44/1/11490/htmlhalotolerant bacteriaplant growth promotionsalt stresssalt resistancewheat cultivars |
| spellingShingle | Eman Hamada Adel Eltoukhy Metwally K. Mahfouz Abdel Kareem S.H. Mohamed Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment Novel Research in Microbiology Journal halotolerant bacteria plant growth promotion salt stress salt resistance wheat cultivars |
| title | Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment |
| title_full | Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment |
| title_fullStr | Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment |
| title_full_unstemmed | Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment |
| title_short | Mitigation of Salt Stress in Two Wheat Cultivars by Promising PGP Halotolerant Bacillus sp. Strain AE-EH1 Isolated from a Marine Environment |
| title_sort | mitigation of salt stress in two wheat cultivars by promising pgp halotolerant bacillus sp strain ae eh1 isolated from a marine environment |
| topic | halotolerant bacteria plant growth promotion salt stress salt resistance wheat cultivars |
| url | https://researcherslinks.com/current-issues/Mitigation-of-Salt-Stress-in-Two-Wheat/44/1/11490/html |
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