Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer
Oral squamous cell carcinoma is the most aggressive cancer that is associated with high recurrence, metastasis, and poor treatment outcome. Dysregulation of long non-coding RNAs has been shown to promote tumor growth and metastasis in several cancers. In this study, we investigated the expression of...
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317698366 |
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| author | Ganesan Arunkumar Arunagiri Kuha Deva Magendhra Rao Mayakannan Manikandan Kanagaraj Arun Vilvanathan Vinothkumar Sundaramoorthy Revathidevi Kottayasamy Seenivasagam Rajkumar Ramamurthy Rajaraman Arasambattu Kannan Munirajan |
| author_facet | Ganesan Arunkumar Arunagiri Kuha Deva Magendhra Rao Mayakannan Manikandan Kanagaraj Arun Vilvanathan Vinothkumar Sundaramoorthy Revathidevi Kottayasamy Seenivasagam Rajkumar Ramamurthy Rajaraman Arasambattu Kannan Munirajan |
| author_sort | Ganesan Arunkumar |
| collection | DOAJ |
| description | Oral squamous cell carcinoma is the most aggressive cancer that is associated with high recurrence, metastasis, and poor treatment outcome. Dysregulation of long non-coding RNAs has been shown to promote tumor growth and metastasis in several cancers. In this study, we investigated the expression of 11 selected long non-coding RNAs that are associated with cell proliferation, metastasis, and tumor suppression in oral squamous cell carcinomas and normal tissues by quantitative real-time polymerase chain reaction. Out of the 11 long non-coding RNAs profiled, 9 were significantly overexpressed in tumors with tobacco chewing history. Moreover, the long non-coding RNA profile was similar to the head and neck cancer datasets of The Cancer Genome Atlas database. Linc-RoR, a regulator of reprogramming, implicated in tumorigenesis was found to be overexpressed in undifferentiated tumors and showed strong association with tumor recurrence and poor therapeutic response. In oral squamous cell carcinomas, for the first time, we observed linc-RoR overexpression, downregulation of miR-145-5p, and overexpression of c-Myc, Klf4, Oct4, and Sox2, suggesting the existence of linc-RoR-mediated competing endogenous RNA network in undifferentiated tumors. Taken together, this study demonstrated the association of linc-RoR overexpression in undifferentiated oral tumors and its prognostic value to predict the therapeutic response. |
| format | Article |
| id | doaj-art-e2cdef61806745f29daf35f36d924ee0 |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-e2cdef61806745f29daf35f36d924ee02025-08-20T03:39:27ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317698366Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancerGanesan Arunkumar0Arunagiri Kuha Deva Magendhra Rao1Mayakannan Manikandan2Kanagaraj Arun3Vilvanathan Vinothkumar4Sundaramoorthy Revathidevi5Kottayasamy Seenivasagam Rajkumar6Ramamurthy Rajaraman7Arasambattu Kannan Munirajan8Department of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaCenter for Oncology, Royapettah Government Hospital & Kilpauk Medical College, Chennai, IndiaCenter for Oncology, Royapettah Government Hospital & Kilpauk Medical College, Chennai, IndiaDepartment of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, IndiaOral squamous cell carcinoma is the most aggressive cancer that is associated with high recurrence, metastasis, and poor treatment outcome. Dysregulation of long non-coding RNAs has been shown to promote tumor growth and metastasis in several cancers. In this study, we investigated the expression of 11 selected long non-coding RNAs that are associated with cell proliferation, metastasis, and tumor suppression in oral squamous cell carcinomas and normal tissues by quantitative real-time polymerase chain reaction. Out of the 11 long non-coding RNAs profiled, 9 were significantly overexpressed in tumors with tobacco chewing history. Moreover, the long non-coding RNA profile was similar to the head and neck cancer datasets of The Cancer Genome Atlas database. Linc-RoR, a regulator of reprogramming, implicated in tumorigenesis was found to be overexpressed in undifferentiated tumors and showed strong association with tumor recurrence and poor therapeutic response. In oral squamous cell carcinomas, for the first time, we observed linc-RoR overexpression, downregulation of miR-145-5p, and overexpression of c-Myc, Klf4, Oct4, and Sox2, suggesting the existence of linc-RoR-mediated competing endogenous RNA network in undifferentiated tumors. Taken together, this study demonstrated the association of linc-RoR overexpression in undifferentiated oral tumors and its prognostic value to predict the therapeutic response.https://doi.org/10.1177/1010428317698366 |
| spellingShingle | Ganesan Arunkumar Arunagiri Kuha Deva Magendhra Rao Mayakannan Manikandan Kanagaraj Arun Vilvanathan Vinothkumar Sundaramoorthy Revathidevi Kottayasamy Seenivasagam Rajkumar Ramamurthy Rajaraman Arasambattu Kannan Munirajan Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer Tumor Biology |
| title | Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer |
| title_full | Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer |
| title_fullStr | Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer |
| title_full_unstemmed | Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer |
| title_short | Expression profiling of long non-coding RNA identifies linc-RoR as a prognostic biomarker in oral cancer |
| title_sort | expression profiling of long non coding rna identifies linc ror as a prognostic biomarker in oral cancer |
| url | https://doi.org/10.1177/1010428317698366 |
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