Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy

Purpose. To characterize hallmark diabetic retinopathy (DR) lesions utilizing adaptive optics scanning laser ophthalmoscopy (AOSLO) and to compare AOSLO findings with those on standard imaging techniques. Methods. Cross-sectional study including 35 eyes of 34 study participants. AOSLO confocal and m...

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Main Authors: Sonja G. Karst, Jan Lammer, Salma H. Radwan, Hanna Kwak, Paolo S. Silva, Stephen A. Burns, Lloyd Paul Aiello, Jennifer K. Sun
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2018/7492946
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author Sonja G. Karst
Jan Lammer
Salma H. Radwan
Hanna Kwak
Paolo S. Silva
Stephen A. Burns
Lloyd Paul Aiello
Jennifer K. Sun
author_facet Sonja G. Karst
Jan Lammer
Salma H. Radwan
Hanna Kwak
Paolo S. Silva
Stephen A. Burns
Lloyd Paul Aiello
Jennifer K. Sun
author_sort Sonja G. Karst
collection DOAJ
description Purpose. To characterize hallmark diabetic retinopathy (DR) lesions utilizing adaptive optics scanning laser ophthalmoscopy (AOSLO) and to compare AOSLO findings with those on standard imaging techniques. Methods. Cross-sectional study including 35 eyes of 34 study participants. AOSLO confocal and multiply scattered light (MSL) imaging were performed in eyes with DR. Color fundus photographs (CF), infrared images of the macula (Spectralis, Heidelberg), and Spectralis spectral domain optical coherence tomography SDOCT B-scans of each lesion were obtained and registered to corresponding AOSLO images. Main Outcome Measures. Individual lesion characterization by AOSLO imaging. AOSLO appearance was compared with CF and SDOCT imaging. Results. Characterized lesions encompassed 52 microaneurysms (MA), 20 intraretinal microvascular abnormalities (IRMA), 7 neovascularization (NV), 11 hard exudates (HE), 5 dot/blot hemorrhages (HEM), 4 cotton wool spots (CWS), and 14 intraretinal cysts. AOSLO allowed assessment of perfusion in vascular lesions and enabled the identification of vascular lesions that could not be visualized on CF or SDOCT. Conclusions. AOSLO imaging provides detailed, noninvasive in vivo visualization of DR lesions enhancing the assessment of morphological characteristics. These unique AOSLO attributes may enable new insights into the pathological changes of DR in response to disease onset, development, regression, and response to therapy.
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spelling doaj-art-e2cc62844fcd4339b7f1c951f7a366682025-08-20T03:21:06ZengWileyInternational Journal of Endocrinology1687-83371687-83452018-01-01201810.1155/2018/74929467492946Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser OphthalmoscopySonja G. Karst0Jan Lammer1Salma H. Radwan2Hanna Kwak3Paolo S. Silva4Stephen A. Burns5Lloyd Paul Aiello6Jennifer K. Sun7Beetham Eye Institute, Joslin Diabetes Center, Boston, MA, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USASchool of Optometry, Indiana University, Bloomington, IN, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USABeetham Eye Institute, Joslin Diabetes Center, Boston, MA, USAPurpose. To characterize hallmark diabetic retinopathy (DR) lesions utilizing adaptive optics scanning laser ophthalmoscopy (AOSLO) and to compare AOSLO findings with those on standard imaging techniques. Methods. Cross-sectional study including 35 eyes of 34 study participants. AOSLO confocal and multiply scattered light (MSL) imaging were performed in eyes with DR. Color fundus photographs (CF), infrared images of the macula (Spectralis, Heidelberg), and Spectralis spectral domain optical coherence tomography SDOCT B-scans of each lesion were obtained and registered to corresponding AOSLO images. Main Outcome Measures. Individual lesion characterization by AOSLO imaging. AOSLO appearance was compared with CF and SDOCT imaging. Results. Characterized lesions encompassed 52 microaneurysms (MA), 20 intraretinal microvascular abnormalities (IRMA), 7 neovascularization (NV), 11 hard exudates (HE), 5 dot/blot hemorrhages (HEM), 4 cotton wool spots (CWS), and 14 intraretinal cysts. AOSLO allowed assessment of perfusion in vascular lesions and enabled the identification of vascular lesions that could not be visualized on CF or SDOCT. Conclusions. AOSLO imaging provides detailed, noninvasive in vivo visualization of DR lesions enhancing the assessment of morphological characteristics. These unique AOSLO attributes may enable new insights into the pathological changes of DR in response to disease onset, development, regression, and response to therapy.http://dx.doi.org/10.1155/2018/7492946
spellingShingle Sonja G. Karst
Jan Lammer
Salma H. Radwan
Hanna Kwak
Paolo S. Silva
Stephen A. Burns
Lloyd Paul Aiello
Jennifer K. Sun
Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
International Journal of Endocrinology
title Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
title_full Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
title_fullStr Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
title_full_unstemmed Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
title_short Characterization of In Vivo Retinal Lesions of Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
title_sort characterization of in vivo retinal lesions of diabetic retinopathy using adaptive optics scanning laser ophthalmoscopy
url http://dx.doi.org/10.1155/2018/7492946
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