Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps
After removal of metastatic malignant tumors of the hypopharynx and larynx, hypopharyngeal defects are formed. To restore the hypopharynx, a mucosa and a muscular component are needed.The objective of this study is to develop a hypopharyngeal reconstruction technique using prelaminated pectoralis ma...
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| Format: | Article |
| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2022-12-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/1543 |
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| author | I. V. Rebrikova E. A. Vorotelyak O. S. Rogovaya A. P. Polyakov A. V. Mordovskiy M. V. Ratushny A. D Kaprin A. V. Vasiliev |
| author_facet | I. V. Rebrikova E. A. Vorotelyak O. S. Rogovaya A. P. Polyakov A. V. Mordovskiy M. V. Ratushny A. D Kaprin A. V. Vasiliev |
| author_sort | I. V. Rebrikova |
| collection | DOAJ |
| description | After removal of metastatic malignant tumors of the hypopharynx and larynx, hypopharyngeal defects are formed. To restore the hypopharynx, a mucosa and a muscular component are needed.The objective of this study is to develop a hypopharyngeal reconstruction technique using prelaminated pectoralis major flap with mucosal epithelium analogue from autologous epithelial layers.Materials and methods. Nine patients underwent reconstruction of the hypopharynx using bioengineered prelaminated pectoralis major flaps. The mucosa was restored by tissue-engineered autologous epithelial cell layers that were obtained by culturing in vitro cells isolated from skin biopsies that were previously obtained from patients.Results. Oral nutrition was restored in all cases. Pharyngeal stenosis was detected in one (11%) patient. A stratified squamous epithelium on the pectoral fascia was revealed in 67% of cases at week 2 after prelamination, in 89% of cases at week 4 after reconstruction and in 100% of cases at month 3, 6, 12 and 24 after reconstruction.Conclusion. Reconstruction using prelaminated bioengineered flaps allows recreating the anatomical integrity and function of the hypopharynx. |
| format | Article |
| id | doaj-art-e2c3eec47c9549dd99126a5f7d961ff4 |
| institution | DOAJ |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2022-12-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-e2c3eec47c9549dd99126a5f7d961ff42025-08-20T03:20:51ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912022-12-0124413514410.15825/1995-1191-2022-4-135-1441133Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flapsI. V. Rebrikova0E. A. Vorotelyak1O. S. Rogovaya2A. P. Polyakov3A. V. Mordovskiy4M. V. Ratushny5A. D Kaprin6A. V. Vasiliev7National Medical Research Radiological Centre; RUDN UniversityKoltzov Institute of Developmental BiologyKoltzov Institute of Developmental BiologyNational Medical Research Radiological Centre; RUDN UniversityNational Medical Research Radiological CentreNational Medical Research Radiological CentreNational Medical Research Radiological CentreKoltzov Institute of Developmental BiologyAfter removal of metastatic malignant tumors of the hypopharynx and larynx, hypopharyngeal defects are formed. To restore the hypopharynx, a mucosa and a muscular component are needed.The objective of this study is to develop a hypopharyngeal reconstruction technique using prelaminated pectoralis major flap with mucosal epithelium analogue from autologous epithelial layers.Materials and methods. Nine patients underwent reconstruction of the hypopharynx using bioengineered prelaminated pectoralis major flaps. The mucosa was restored by tissue-engineered autologous epithelial cell layers that were obtained by culturing in vitro cells isolated from skin biopsies that were previously obtained from patients.Results. Oral nutrition was restored in all cases. Pharyngeal stenosis was detected in one (11%) patient. A stratified squamous epithelium on the pectoral fascia was revealed in 67% of cases at week 2 after prelamination, in 89% of cases at week 4 after reconstruction and in 100% of cases at month 3, 6, 12 and 24 after reconstruction.Conclusion. Reconstruction using prelaminated bioengineered flaps allows recreating the anatomical integrity and function of the hypopharynx.https://journal.transpl.ru/vtio/article/view/1543hypopharyngeal defectsreconstructionprelaminated pectoralis major flaptissue engineeringepithelial layerskeratinocytes |
| spellingShingle | I. V. Rebrikova E. A. Vorotelyak O. S. Rogovaya A. P. Polyakov A. V. Mordovskiy M. V. Ratushny A. D Kaprin A. V. Vasiliev Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps Вестник трансплантологии и искусственных органов hypopharyngeal defects reconstruction prelaminated pectoralis major flap tissue engineering epithelial layers keratinocytes |
| title | Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps |
| title_full | Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps |
| title_fullStr | Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps |
| title_full_unstemmed | Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps |
| title_short | Hypopharyngeal reconstruction using prelaminated autologous bio-engineered pectoralis major flaps |
| title_sort | hypopharyngeal reconstruction using prelaminated autologous bio engineered pectoralis major flaps |
| topic | hypopharyngeal defects reconstruction prelaminated pectoralis major flap tissue engineering epithelial layers keratinocytes |
| url | https://journal.transpl.ru/vtio/article/view/1543 |
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