EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI

The study's goals are to use pyocyanin pigment produced by the clinical isolate Pseudomonas aeruginosa as a reducing and stabilizing agent for nickel oxide nanoparticles (NiO NPs) and their use it antibacterial agents against biofilm-producing, multidrug-resistant Escherichia coli isolated fro...

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Main Authors: H. O. Ahmed, L. A. Yaaqoob
Format: Article
Language:English
Published: Baghdad University 2025-02-01
Series:The Iraqi Journal of Agricultural science
Subjects:
Online Access:https://www.jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/2164
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author H. O. Ahmed
L. A. Yaaqoob
author_facet H. O. Ahmed
L. A. Yaaqoob
author_sort H. O. Ahmed
collection DOAJ
description The study's goals are to use pyocyanin pigment produced by the clinical isolate Pseudomonas aeruginosa as a reducing and stabilizing agent for nickel oxide nanoparticles (NiO NPs) and their use it antibacterial agents against biofilm-producing, multidrug-resistant Escherichia coli isolated from various clinical sources. The antibiotic susceptibility test of E. Coli isolates was shown to be resistant to ceftriaxone, cotrimoxazole, piperacillin-triazole, tetracycline, and ticarcillin, while sensitive to Amikacin, Amoxicillin, Nitrofurantoin, and Imipenem. NiO NPs are synthesized by using nickel sulfate NiSO4 (10g) with a concentration of pyocyanin (10mg/10ml). The NiO NPs synthesized were characterized by various techniques such as AFM, UV-VIS, and FTIR. The result showed that the wavelength of NiO was 211nm and the average diameter of NiO was 63.59 nm, and the concentration of NiO NPs was 0.5 mg/ml, showing that the maximum inhibition zones of E.coli were 22 mm. Biosynthesis of NiO NPs using pyocyanin was shown to have promising activity as an antibacterial against the biofilm-producing E. coli.
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issn 0075-0530
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series The Iraqi Journal of Agricultural science
spelling doaj-art-e2be9ece4a9e4d878a0e4ead921826aa2025-08-20T02:04:30ZengBaghdad UniversityThe Iraqi Journal of Agricultural science0075-05302410-08622025-02-0156110.36103/aws0zt84EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLIH. O. AhmedL. A. Yaaqoob The study's goals are to use pyocyanin pigment produced by the clinical isolate Pseudomonas aeruginosa as a reducing and stabilizing agent for nickel oxide nanoparticles (NiO NPs) and their use it antibacterial agents against biofilm-producing, multidrug-resistant Escherichia coli isolated from various clinical sources. The antibiotic susceptibility test of E. Coli isolates was shown to be resistant to ceftriaxone, cotrimoxazole, piperacillin-triazole, tetracycline, and ticarcillin, while sensitive to Amikacin, Amoxicillin, Nitrofurantoin, and Imipenem. NiO NPs are synthesized by using nickel sulfate NiSO4 (10g) with a concentration of pyocyanin (10mg/10ml). The NiO NPs synthesized were characterized by various techniques such as AFM, UV-VIS, and FTIR. The result showed that the wavelength of NiO was 211nm and the average diameter of NiO was 63.59 nm, and the concentration of NiO NPs was 0.5 mg/ml, showing that the maximum inhibition zones of E.coli were 22 mm. Biosynthesis of NiO NPs using pyocyanin was shown to have promising activity as an antibacterial against the biofilm-producing E. coli. https://www.jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/2164biofilm, antimicrobial activity, pyocyanin, E.coli, Nickel oxide nanoparticles (NiO NPs).
spellingShingle H. O. Ahmed
L. A. Yaaqoob
EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
The Iraqi Journal of Agricultural science
biofilm, antimicrobial activity, pyocyanin, E.coli, Nickel oxide nanoparticles (NiO NPs).
title EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
title_full EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
title_fullStr EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
title_full_unstemmed EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
title_short EVALUATION OF ANTIBACTERIAL ACTIVITY OF NICKEL OXIDE NANOPARTICLES AGAINST ESCHERICHIA COLI
title_sort evaluation of antibacterial activity of nickel oxide nanoparticles against escherichia coli
topic biofilm, antimicrobial activity, pyocyanin, E.coli, Nickel oxide nanoparticles (NiO NPs).
url https://www.jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/2164
work_keys_str_mv AT hoahmed evaluationofantibacterialactivityofnickeloxidenanoparticlesagainstescherichiacoli
AT layaaqoob evaluationofantibacterialactivityofnickeloxidenanoparticlesagainstescherichiacoli