Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms
Three simple, precise and cost-effective spectrophotometric methods have been developed for the determination of Mycophenolate in bulk and its pharmaceutical formulations. Mycophenolate shows 𝜆max at 250.0 nm in zero-derivative spectrum (method A), 258.0 nm in first-derivative spectrum (method B) an...
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| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/523193 |
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| author | A. Narendra D. Deepika M. Mathrusri Annapurna |
| author_facet | A. Narendra D. Deepika M. Mathrusri Annapurna |
| author_sort | A. Narendra |
| collection | DOAJ |
| description | Three simple, precise and cost-effective spectrophotometric methods have been developed for the determination of Mycophenolate in bulk and its pharmaceutical formulations. Mycophenolate shows 𝜆max at 250.0 nm in zero-derivative spectrum (method A), 258.0 nm in first-derivative spectrum (method B) and method C is based on the calculation of area under curve (AUC) for analysis of Mycophenolate in the wavelength range of 240.0–260.0 nm. The drug follows the Beer-Lambert's law in the concentration range of 1.0–150.0 μg/mL for all the methods. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Mycophenolate in bulk and pharmaceutical dosage forms. |
| format | Article |
| id | doaj-art-e2b837840c3642f6bb018eb8d0d963d0 |
| institution | DOAJ |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-e2b837840c3642f6bb018eb8d0d963d02025-08-20T03:21:07ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/523193523193Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage FormsA. Narendra0D. Deepika1M. Mathrusri Annapurna2Department of Pharmaceutical Analysis and Quality Assurance, Roland Institute of Pharmaceutical Sciences, Berhampur 760010, IndiaDepartment of Pharmaceutical Analysis and Quality Assurance, Royal College of Pharmacy and Health Sciences, Berhampur 760002, IndiaDepartment of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam 530045, IndiaThree simple, precise and cost-effective spectrophotometric methods have been developed for the determination of Mycophenolate in bulk and its pharmaceutical formulations. Mycophenolate shows 𝜆max at 250.0 nm in zero-derivative spectrum (method A), 258.0 nm in first-derivative spectrum (method B) and method C is based on the calculation of area under curve (AUC) for analysis of Mycophenolate in the wavelength range of 240.0–260.0 nm. The drug follows the Beer-Lambert's law in the concentration range of 1.0–150.0 μg/mL for all the methods. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Mycophenolate in bulk and pharmaceutical dosage forms.http://dx.doi.org/10.1155/2013/523193 |
| spellingShingle | A. Narendra D. Deepika M. Mathrusri Annapurna Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms Journal of Chemistry |
| title | Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms |
| title_full | Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms |
| title_fullStr | Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms |
| title_full_unstemmed | Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms |
| title_short | Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms |
| title_sort | validated spectrophotometric methods for the determination of mycophenolate an anti neoplastic agent in bulk and pharmaceutical dosage forms |
| url | http://dx.doi.org/10.1155/2013/523193 |
| work_keys_str_mv | AT anarendra validatedspectrophotometricmethodsforthedeterminationofmycophenolateanantineoplasticagentinbulkandpharmaceuticaldosageforms AT ddeepika validatedspectrophotometricmethodsforthedeterminationofmycophenolateanantineoplasticagentinbulkandpharmaceuticaldosageforms AT mmathrusriannapurna validatedspectrophotometricmethodsforthedeterminationofmycophenolateanantineoplasticagentinbulkandpharmaceuticaldosageforms |