Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages
Constructions of self-assembled protein nanocages for enzyme immobilization and cargo transport are very promising in biotechnology fields such as natural product biosynthesis. Here, we present an engineered isopentenyl pyrophosphate (IPP) synthetic nanocage with multiple enzymes for lycopene produc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/4/747 |
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| author | Yulong Zhou Yonghua Yao Furong Zhang Ning Yu Binqiang Wang Bing Tian |
| author_facet | Yulong Zhou Yonghua Yao Furong Zhang Ning Yu Binqiang Wang Bing Tian |
| author_sort | Yulong Zhou |
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| description | Constructions of self-assembled protein nanocages for enzyme immobilization and cargo transport are very promising in biotechnology fields such as natural product biosynthesis. Here, we present an engineered isopentenyl pyrophosphate (IPP) synthetic nanocage with multiple enzymes for lycopene production in bacteria. The enzymes involved in IPP biosynthesis (ScCK, AtIPK, and MxanIDI) were assembled onto the exterior of an engineered protein cage based on α-carboxysome. The IPP synthetic nanocage was co-expressed with CrtE/CrtB/CrtI in <i>Escherichia coli</i>. This approach increased the metabolic flux and resulted in a 1.7-fold increase in lycopene production in the engineered <i>E</i>. <i>coli</i> compared with the control strain. The results provide insights into the immobilization and assembling of IPP biosynthetic enzymes in protein nanocages, which serve as a powerful tool for achieving efficient synthesis of lycopene. |
| format | Article |
| id | doaj-art-e2b412ca1e584643a74891a5c8ff39c2 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-e2b412ca1e584643a74891a5c8ff39c22025-08-20T02:18:14ZengMDPI AGMicroorganisms2076-26072025-03-0113474710.3390/microorganisms13040747Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein CagesYulong Zhou0Yonghua Yao1Furong Zhang2Ning Yu3Binqiang Wang4Bing Tian5Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, ChinaKey Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, ChinaInstitute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, ChinaConstructions of self-assembled protein nanocages for enzyme immobilization and cargo transport are very promising in biotechnology fields such as natural product biosynthesis. Here, we present an engineered isopentenyl pyrophosphate (IPP) synthetic nanocage with multiple enzymes for lycopene production in bacteria. The enzymes involved in IPP biosynthesis (ScCK, AtIPK, and MxanIDI) were assembled onto the exterior of an engineered protein cage based on α-carboxysome. The IPP synthetic nanocage was co-expressed with CrtE/CrtB/CrtI in <i>Escherichia coli</i>. This approach increased the metabolic flux and resulted in a 1.7-fold increase in lycopene production in the engineered <i>E</i>. <i>coli</i> compared with the control strain. The results provide insights into the immobilization and assembling of IPP biosynthetic enzymes in protein nanocages, which serve as a powerful tool for achieving efficient synthesis of lycopene.https://www.mdpi.com/2076-2607/13/4/747carboxysomeenzyme immobilizationIPP synthetic nanocagelycopene production |
| spellingShingle | Yulong Zhou Yonghua Yao Furong Zhang Ning Yu Binqiang Wang Bing Tian Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages Microorganisms carboxysome enzyme immobilization IPP synthetic nanocage lycopene production |
| title | Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages |
| title_full | Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages |
| title_fullStr | Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages |
| title_full_unstemmed | Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages |
| title_short | Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages |
| title_sort | enhancement of lycopene biosynthesis using self assembled multi enzymic protein cages |
| topic | carboxysome enzyme immobilization IPP synthetic nanocage lycopene production |
| url | https://www.mdpi.com/2076-2607/13/4/747 |
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