The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study
Aim. To evaluate the impact of subanesthetic concentrations of xenon on the brain levels of GSK-3β, NF-κB, and Nrf2 in intact rats.Materials and Methods. Male laboratory rats were randomly assigned to three groups (N=5 per group): the control group received inhalation of a nitrogen-oxygen gas mixtur...
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2025-06-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/2594 |
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| author | E. E. Beda M. V. Gabitov I. V. Redkin I. A. Kryukov O. A. Grebenchikov |
| author_facet | E. E. Beda M. V. Gabitov I. V. Redkin I. A. Kryukov O. A. Grebenchikov |
| author_sort | E. E. Beda |
| collection | DOAJ |
| description | Aim. To evaluate the impact of subanesthetic concentrations of xenon on the brain levels of GSK-3β, NF-κB, and Nrf2 in intact rats.Materials and Methods. Male laboratory rats were randomly assigned to three groups (N=5 per group): the control group received inhalation of a nitrogen-oxygen gas mixture; the Xe-70 group received 70% xenon; and the Xe-35 group received 35% xenon. Following euthanasia, brain tissue samples were analyzed using Western blotting and densitometric quantification to assess levels of phosphorylated GSK-3β, NF-κB, and Nrf2. Results. Inhalation of xenon-oxygen mixtures led to a statistically significant increase in phosphorylated GSK-3β levels in both the Xe-70 group (95% CI: 593,723–1,018,826; P=0.0001; R=0.72) and the Xe-35 group (95% CI: 458,413–872,807; P=0.0001; R=0.80), compared with controls. Xenon exposure also resulted in a significant reduction in NF-κB levels in the Xe-70 (95% CI: 205,138–601,617; P=0.0005; R=0.95) and Xe-35 (95% CI: 217,700–608,462; P=0.0003; R=0.95) groups. Furthermore, Nrf2 protein expression was significantly elevated in the Xe-35 group compared to controls (95% CI: 260,926–692,532; P=0.0002; R=0.91).Conclusion. Subanesthetic xenon concentrations exert a significant modulatory effect on GSK-3β, NF-κB, and Nrf2 expression in the brain tissue of intact rats. |
| format | Article |
| id | doaj-art-e2b36e1ee5c04927a34f75dbbe195153 |
| institution | Kabale University |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-e2b36e1ee5c04927a34f75dbbe1951532025-08-20T03:35:08ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102025-06-01213263110.15360/1813-9779-2025-3-25632003The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental StudyE. E. Beda0M. V. Gabitov1I. V. Redkin2I. A. Kryukov3O. A. Grebenchikov4V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyAim. To evaluate the impact of subanesthetic concentrations of xenon on the brain levels of GSK-3β, NF-κB, and Nrf2 in intact rats.Materials and Methods. Male laboratory rats were randomly assigned to three groups (N=5 per group): the control group received inhalation of a nitrogen-oxygen gas mixture; the Xe-70 group received 70% xenon; and the Xe-35 group received 35% xenon. Following euthanasia, brain tissue samples were analyzed using Western blotting and densitometric quantification to assess levels of phosphorylated GSK-3β, NF-κB, and Nrf2. Results. Inhalation of xenon-oxygen mixtures led to a statistically significant increase in phosphorylated GSK-3β levels in both the Xe-70 group (95% CI: 593,723–1,018,826; P=0.0001; R=0.72) and the Xe-35 group (95% CI: 458,413–872,807; P=0.0001; R=0.80), compared with controls. Xenon exposure also resulted in a significant reduction in NF-κB levels in the Xe-70 (95% CI: 205,138–601,617; P=0.0005; R=0.95) and Xe-35 (95% CI: 217,700–608,462; P=0.0003; R=0.95) groups. Furthermore, Nrf2 protein expression was significantly elevated in the Xe-35 group compared to controls (95% CI: 260,926–692,532; P=0.0002; R=0.91).Conclusion. Subanesthetic xenon concentrations exert a significant modulatory effect on GSK-3β, NF-κB, and Nrf2 expression in the brain tissue of intact rats.https://www.reanimatology.com/rmt/article/view/2594xenongsk-3βnf-κbnrf2brainrats |
| spellingShingle | E. E. Beda M. V. Gabitov I. V. Redkin I. A. Kryukov O. A. Grebenchikov The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study Общая реаниматология xenon gsk-3β nf-κb nrf2 brain rats |
| title | The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study |
| title_full | The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study |
| title_fullStr | The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study |
| title_full_unstemmed | The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study |
| title_short | The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study |
| title_sort | effects of xenon on gsk 3β nf κb and nrf2 levels in the rat brain an experimental study |
| topic | xenon gsk-3β nf-κb nrf2 brain rats |
| url | https://www.reanimatology.com/rmt/article/view/2594 |
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