The Effects of Xenon on GSK-3β, NF-κB, and Nrf2 Levels in the Rat Brain: An Experimental Study
Aim. To evaluate the impact of subanesthetic concentrations of xenon on the brain levels of GSK-3β, NF-κB, and Nrf2 in intact rats.Materials and Methods. Male laboratory rats were randomly assigned to three groups (N=5 per group): the control group received inhalation of a nitrogen-oxygen gas mixtur...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2025-06-01
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| Series: | Общая реаниматология |
| Subjects: | |
| Online Access: | https://www.reanimatology.com/rmt/article/view/2594 |
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| Summary: | Aim. To evaluate the impact of subanesthetic concentrations of xenon on the brain levels of GSK-3β, NF-κB, and Nrf2 in intact rats.Materials and Methods. Male laboratory rats were randomly assigned to three groups (N=5 per group): the control group received inhalation of a nitrogen-oxygen gas mixture; the Xe-70 group received 70% xenon; and the Xe-35 group received 35% xenon. Following euthanasia, brain tissue samples were analyzed using Western blotting and densitometric quantification to assess levels of phosphorylated GSK-3β, NF-κB, and Nrf2. Results. Inhalation of xenon-oxygen mixtures led to a statistically significant increase in phosphorylated GSK-3β levels in both the Xe-70 group (95% CI: 593,723–1,018,826; P=0.0001; R=0.72) and the Xe-35 group (95% CI: 458,413–872,807; P=0.0001; R=0.80), compared with controls. Xenon exposure also resulted in a significant reduction in NF-κB levels in the Xe-70 (95% CI: 205,138–601,617; P=0.0005; R=0.95) and Xe-35 (95% CI: 217,700–608,462; P=0.0003; R=0.95) groups. Furthermore, Nrf2 protein expression was significantly elevated in the Xe-35 group compared to controls (95% CI: 260,926–692,532; P=0.0002; R=0.91).Conclusion. Subanesthetic xenon concentrations exert a significant modulatory effect on GSK-3β, NF-κB, and Nrf2 expression in the brain tissue of intact rats. |
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| ISSN: | 1813-9779 2411-7110 |